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A pregnancy test strip for detection of pathogenic bacteria by using concanavalin A-human chorionic gonadotropin-Cu3(PO4)2 hybrid nanoflowers, magnetic separation, and smartphone readout

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A Correction to this article was published on 03 October 2018

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Abstract

Pregnancy test strips are widely used in daily life. A commercial pregnancy test strip was modified to obtain a point-of-care device for the detection of pathogenic bacteria. Hybrid nanoflowers were prepared from concanavalin A, human chorionic gonadotropin, and Cu3(PO4)2 via a one-pot method. They were used as signaling probes in an off-the-shelf pregnancy test strip. This modified lateral flow immunoassay can detect Escherichia coli O157:H7 with a detection limit of 4 CFU·mL−1, and Salmonella typhimurium with a detection limit of 3 CFU·mL−1. Conceivably, the method has high potential as a portable and cost-effective tool for rapid determination of a wide range of analytes, especially in resource-constrained settings.

Hybrid nanoflower loaded human chorionic gonadotropin (hCG) and concanavalin A (hCG − nanoflowers) were synthesized via a one-pot method and used as signal labels with commercial commercial-off-the-shelf pregnancy test strips to detect pathogenic bacteria targets, thus yielding an easily smartphone readout signal.

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  • 03 October 2018

    The published version of this article, unfortunately, contained error. The authors are re-writing to express their sincere apology for a mistake that a mark “10-5, 10-4, 10-3, 10-2, 10-1 CFU•mL-1” in the legend of Fig. 2 was not corrected as “105, 104, 103, 102, 101 CFU•mL-1”.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (No. 2016YFD0501001).

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Author notes

  1. Shengjun Bu and Kuiyu Wang contributed equally to this work.

Authors and Affiliations

  1. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, 130122, China

    Shengjun Bu, Kuiyu Wang, Zhongyi Li, Peng Du, Zhuo Hao, Wensen Liu & Jiayu Wan

  2. The General Hospital of FAW, Changchun, 130011, China

    Chuanjing Ju

  3. The Fourth Hospital of Jilin University, Changchun, 130011, China

    Chuanjing Ju

  4. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China

    Ye Han

  5. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, China

    Changtian Li

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  1. Shengjun Bu
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Correspondence to Wensen Liu or Jiayu Wan.

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Bu, S., Wang, K., Ju, C. et al. A pregnancy test strip for detection of pathogenic bacteria by using concanavalin A-human chorionic gonadotropin-Cu3(PO4)2 hybrid nanoflowers, magnetic separation, and smartphone readout. Microchim Acta 185, 464 (2018). https://doi.org/10.1007/s00604-018-2968-2

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