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Radioprotective efficacy of dieckol against gamma radiation-induced cellular damage in hepatocyte cells

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Abstract

Naturally occurring antioxidants prevent or delay the harmful effect of free radical formation and radioprotection. The present study aimed to investigate the radioprotective effect of dieckol, a naturally occurring marine bioactive phenolic compound on lipid peroxidation and antioxidant status, DNA damage, and inflammation in gamma-radiation-induced rat primary hepatocytes. Isolated hepatocyte cells exposed to gamma-radiation showed an increased level of lipid peroxidation markers (thiobarbituric acid reactive substances and lipid hydroperoxides) accompanied with the decrease in the activities of enzymatic (SOD, CAT, and GPx) and non-enzymatic (vitamin C, vitamin E, and GSH) antioxidants associated with increased DNA damage coupled with upregulation of inflammatory proteins (NF-κB and COX-2) compared to control. Treatment of dieckol (5, 10, 20 μM) reduces the γ-radiation-induced toxicity and the associated pro-oxidant and antioxidant imbalance as well as decreasing the DNA damage (tail length, tail moment, %DNA in a tail and olive tail moment) and inflammation in hepatocyte cells. These findings indicate that treatment of dieckol offers protection against γ-radiation-induced cellular damage in the liver cells.

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Author notes

  1. Velayutham Sadeeshkumar and Arul Duraikannu contributed equally to this work.

Authors and Affiliations

  1. Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, 608 502, India

    Velayutham Sadeeshkumar, Samuthrapandian Ravichandran & Raghunathan Ganeshamurthy

  2. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu, 608 002, India

    Velayutham Sadeeshkumar & Arul Duraikannu

  3. Division of Neurology, Department of Medicine & Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada

    Arul Duraikannu

  4. Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Killikulam, Vallanadu, Tamil Nadu, 628 252, India

    Thiyagarajan Aishwarya

  5. Pondicherry Institute of Medical Sciences, Ganapathichettikulam, Kalapet, Puducherry, 605 014, India

    Prithi Jayaram

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  1. Velayutham Sadeeshkumar
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Contributions

V.S. conducted experiments, analyzed data, supervised and wrote the manuscript; A.D. participated equally in the first authorship and designed, conducted experiments, drafted figure, co-wrote the manuscript, and analyzed data; T.A. conducted experiments and analyzed data; P.J. performed experiments, analyzed data and co-wrote the manuscript; S.R. performed experimental approaches and data to the work; R.G. helped to identify and formulate the research and interpreted experiments.

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Correspondence to Velayutham Sadeeshkumar.

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Sadeeshkumar, V., Duraikannu, A., Aishwarya, T. et al. Radioprotective efficacy of dieckol against gamma radiation-induced cellular damage in hepatocyte cells. Naunyn-Schmiedeberg's Arch Pharmacol 392, 1031–1041 (2019). https://doi.org/10.1007/s00210-019-01652-z

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