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Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage

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Abstract

The potential antioxidant activities of three phlorotannins (phloroglucinol, eckol and dieckol) purified from Ecklonia cava collected in Jeju Island were investigated to evaluate their potential value as the natural products for foods or cosmetic application. In this study, antioxidant activities were measured by electron spin resonance spectrometry (ESR) technique for scavenging effects of free radicals such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), alkyl, hydroxyl (HO•) and superoxide anion radical (O2 •−) and by comet assay for protecting effects against H2O2-mediated DNA damage. The results show that all the phlorotannins have the potential DPPH, alkyl, hydroxyl and superoxide radical scavenging activities. Especially, eckol samples scavenged around 93% of DPPH at 0.25, 0.5, 1 mg/mL of concentrations and were higher than the other phlorotannins, such as phloroglucinol and dieckol samples. Also, protecting effects of the phlorotannins against H2O2-mediated DNA damage increased with increased concentrations of the samples in the L5178 mouse T-cell lymphoma cell lines (L5178Y-R). In conclusion, these results suggest that the three phlorotannins purified from E. cava have the potential inhibitory effect on H2O2-mediated DNA damage and harmful free radicals and can be used as antioxidants in cosmetic, foods and drug industry.

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Acknowledgment

This research was supported by a grant (P-2004-03) from the Marine Bioprocess, Research Center of the Marine Bio 21 Center, funded by the Ministry of Maritime Affairs and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Faculty of Applied Marine Science, Cheju National University, Jeju, 690-756, Korea

    Gin-Nae Ahn, Kil-Nam Kim, Seon-Heui Cha, Choon-Bok Song, Jehee Lee, Moon-Soo Heo, In-Kyu Yeo & You-Jin Jeon

  2. Department of Chemistry, Cheju National University, Jeju, 690-756, Korea

    Nam-Ho Lee

  3. Department of Veterinary Medicine, Applied Radiological Science Institute, Cheju National University, Jeju, 690-756, Korea

    Young-Heun Jee

  4. Division of Marine Bioscience, Gyeongsang National University, Seoul, 143-130, Korea

    Jin-Soo Kim & Min-Soo Heu

  5. Applied Radiological Science Research Institute, Cheju National University, Jeju, 690-756, Korea

    You-Jin Jeon

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  1. Gin-Nae Ahn
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  2. Kil-Nam Kim
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Correspondence to You-Jin Jeon.

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Ahn, GN., Kim, KN., Cha, SH. et al. Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage. Eur Food Res Technol 226, 71–79 (2007). https://doi.org/10.1007/s00217-006-0510-y

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  • DOI: https://doi.org/10.1007/s00217-006-0510-y

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