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Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region

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Abstract

A novel cellulase-producing actinomycete strain Gxj-6T, isolated from soil in the cold region (Heihe city, Heilongjiang province, the northernmost part of China), subjected to a taxonomic study using a polyphasic approach. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain Gxj-6T fell within the clade comprising the type strains of species of the genus Microbispora. 16S rRNA gene sequence similarity studies exhibited that species Gxj-6T was most closely related to Microbispora bryophytorum NEAU-TX2-2T (99.45%), Microbispora fusca NEAU-HEGS1-5T (99.41%), Microbispora camponoti 2C-HV3T (99.31%) and Microbispora rosea subsp. rosea JCM 3006T (98.68%). Organism Gxj-6T contained MK-9(H2) as the predominant ubiquinone and C18:0 10-methyl as the major fatty acid. The major polar lipids of culture Gxj-6T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, three unidentified phospholipids, two unidentified glycolipids and two unidentified lipids. The DNA G+C content of strain Gxj-6T was 71.25 mol%. The morphological and chemotaxonomic properties of the strain are also consistent with those members of the genus Microbispora. Combinated with the lower DNA–DNA relatedness values, phenotypic properties, physiology and biochemistry distinctiveness with other recognized species strains, revealed that strain Gxj-6T is separated from other phylogenetically closely species of the genus Microbispora. Therefore, strain Gxj-6T is considered to represent a novel species of the genus Microbispora, for which the name Microbispora cellulosiformans sp. nov. is proposed. The type strain is Gxj-6T (= CGMCC 4.7605T = DSM 109712T).

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Acknowledgements

We would like to thank Professor Wensheng Xiang at the Northeast Agricultural University, People’s Republic of China, for providing us with the similarity strain Microbispora fusca NEAU-HEGS1-5T and Microbispora camponoti 2C-HV3T. This work was supported in part by grants from Harbin applied technology research and development project (NO. 2017RAQYJ142) and Science and technology cooperation project of Heilongjiang Provincial Science and Technology Planning Institute (NO. YS18B14). We are also grateful to Professor Aharon Oren for helpful advice on the specific epithet.

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Authors and Affiliations

  1. College of Land and Environment, Shenyang Agricultural University, No.120, DongLing Road, Shenhe District, Shenyang, 110866, People’s Republic of China

    Xiujie Gong & Hongtao Zou

  2. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Wensheng Xiang

  3. Institute of Microbiology, Heilongjiang Academy of Sciences, No. 68 Zhaolin Street, Daoli District, Harbin, 150086, People’s Republic of China

    Xu Cao

  4. Institute of Farming and Cultivation, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin, 150086, People’s Republic of China

    Xiujie Gong, Yang Yu, Yubo Hao, Liang Li & Chunrong Qian

  5. Institute of Soil Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin, 150086, People’s Republic of China

    Qiuju Wang

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Contributions

XG, HZ, CQ conceived the idea of the study; WX provided the similar strains; XG, XC, YY analysed the data; XG, HZ, YH, LL, QW interpreted the results; XG wrote the paper; all authors discussed the results and revised the manuscript.

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Correspondence to Hongtao Zou or Chunrong Qian.

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The authors declare that they have no conflict of interest. Informed consent was obtained from all individual participants included in the study.

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I certify that this manuscript is original and has not been published and will not be submitted elsewhere for publication while being considered by Hongtao Zou. And the study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time. No data have been fabricated or manipulated (including images) to support our conclusions. The submission has been received explicitly from all co-authors. And authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

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Gong, X., Xiang, W., Cao, X. et al. Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region. Antonie van Leeuwenhoek 113, 2053–2062 (2020). https://doi.org/10.1007/s10482-020-01477-4

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