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Nakamurella leprariae sp. nov., isolated from a lichen sample

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Abstract

A novel actinobacterium, YIM 132084T, was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in color. It grew at 10–40 °C (optimum 28 °C), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4% NaCl (optimum 0%). Strain YIM 132084T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H4) as the predominant menaquinone, and anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0 as major fatty acids. Strain YIM 132084T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127T (97.7%) and Nakamurella flava CGMCC 4.7524T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Based on draft genome sequences, strain YIM 132084T showed an average nucleotide identity value of 76.1% and 74.9%, a digital DNA–DNA hybridization value of 20.9% and 20.6% with the reference strains Nakamurella flavida and Nakamurella flava, respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses showed that strain YIM 132084T represents a novel species of the genus Nakamurella, for which the name Nakamurella leprariae sp. nov. is proposed. The type strain is YIM 132084T (= CGMCC 4.7667T = NBRC 114280T = KCTC 49367T).

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Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

DPG:

Diphosphatidylglycerol

PE:

Phosphatidylethanolamine

PI:

Phosphatidylinositol

APL:

Aminophospholipid

PGL:

Phosphoglycolipids

GL:

Glycolipid

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Acknowledgements

This research was funded by National Natural Science Foundation of China (32060001) and Major research project of Guangxi for science and technology (AA18242026).

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Author notes

  1. De-Feng An and Shao-Juan Yang equally contributed to this work.

Authors and Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan University, Kunming, People’s Republic of China

    De-Feng An, Shao-Juan Yang, Long-Qian Jiang, Xiao-Yu Huang, Lei Lang, Xue-Mei Chen, Ming-Qun Fan, Gui-Ding Li, Cheng-Lin Jiang & Yi Jiang

  2. Key Lab for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Xin-Yu Wang & Li-Song Wang

  3. Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People’s Republic of China

    Gui-Ding Li

  4. School of Marine Science and Biotechnology, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning, 530008, People’s Republic of China

    Ming-Guo Jiang

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  1. De-Feng An
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Contributions

D-FA and S-JY performed the experiments and wrote the manuscript; L-QJ collected the lichen samples; X-YH, X-MC, M-QF, G-DL, and LL analyzed the data; X-YW identified the lichen samples; YJ and M-GJ guided the experiments and revised the manuscript; C-LJ and L-SW designed the study.

Corresponding author

Correspondence to Yi Jiang.

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The authors declare that they have no conflicts of interest.

Additional information

Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM132084T is MZ050064 and the genome sequence is JAERWK000000000.

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An, DF., Yang, SJ., Jiang, LQ. et al. Nakamurella leprariae sp. nov., isolated from a lichen sample. Arch Microbiol 204, 19 (2022). https://doi.org/10.1007/s00203-021-02626-7

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