Abstract
Microcystis growth and physiological responses to chloramphenicol (CAP)-stress were explored at different phosphorus (P) concentrations during 20-day exposure. Under CAP-stress, Microcystis exhibited (i) stronger total protein synthesis and antioxidant defenses at 5 mg/L P than 0.05–0.5 mg/L P in early test period (before day 8), and (ii) greater CAP-removal via biodegradation at 5 mg/L P in mid-late period. Due to above mechanisms, 5 mg/L P largely alleviated the inhibitory effect of CAP on Microcystis growth until test end, thus minimizing CAP toxicity to Microcystis, compared with 0.05–0.5 mg/L P. Moreover, microcystin-production and -release by Microcystis under CAP-stress were also P-dependent. These results suggested that under CAP-stress, although Microcystis growth was more inhibited at 0.05–0.5 mg/L P, higher microcystin-release and CAP residual at 0.05–0.5 mg/L P than at 5 mg/L P still caused eco-risks, which had important implication for risk assessment during Microcystis-dominated blooms and CAP pollution co-occurrence in different waters.
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References
Belchik SM, Xun L (2011) S-glutathionyl-(chloro)hydroquinone reductases: a new class of glutathione transferases functioning as oxidoreductases. Drug Metab Rev 43:307–316
Bradford MA (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Briand E, Yéprémian C, Humbert JF, Quiblier C (2008) Competition between microcystin- and non-microcystin-producing Planktothrix agardhii (cyanobacteria) strains under different environmental conditions. Environ Microbiol 10:3337–3348
Campos A, Vasconcelos V (2010) Molecular mechanisms of microcystin toxicity in animal cells. Int J Mol Sci 11:268–287
Cano-Europa E, Ortiz-ButrÓn R, Gallardo-Casas CA, Blas-Valdivia V, Pineda-Reynoso M, Olvera-Ramírez R, Franco-Colin M (2010) Phycobiliprotein from Pseudanabaena tenuis rich in c-phycoerythrin protect against HgCl2-caused oxidative stress and cellular damage in the kidney. J Appl Phycol 22:495–501
Ding Y, Gan NQ, Zheng LL, Song LR (2013) Microcystin-LR increases the fitness of Microcystis aeruginosa under H2O2 stress. Acta Hydrobiol Sin 37:515–521 (in Chinese)
Draper HH, Hadley M (1990) A review of recent studies on the metabolism of exogenous and endogenous malondialdehyde. Xenobiotica 20:901–907
EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain) (2014) Scientific opinion on chloramphenicol in food and feed. EFSA J 12:3907
Kong QX, Zhu LZ, Shen XY (2010) The toxicity of naphthalene to marine Chlorella vulgaris under different nutrient conditions. J Hazard Mater 178:282–286
Kümmerer K (2009) Antibiotics in the aquatic environment—a review—part I. Chemosphere 75:417–434
Li JM, Shimizu K, Zhou YL, Utsumi M, Sakharkar MK, Zhang ZY, Sun HW, Sugiura N (2011) Biodegradation of microcystins by bacterial communities co-existing with the flagellate Monas guttula and concurrent succession of community structures. J Water Suppl Res Technol AQUA 60:352–363
Li JM, Li J, Shi G, Mei ZL, Wang RP, Li DY (2016) Discerning biodegradation and adsorption of microcystin-LR in a shallow semi-enclosed bay and bacterial community shifts in response to associated process. Ecotoxicol Environ Saf 132:123–131
Li JM, Li RH, Li J (2017) Current research scenario for microcystins biodegradation—a review on fundamental knowledge, application prospects and challenges. Sci Total Environ 595:615–632
Liu H, Zhang GP, Liu CQ, Li L, Xiang M (2009) Characteristics of chloramphenicol and tetracyclines in municipal sewage and Nanming River of Guiyang City, China. Environ Sci 30:687–692 (in Chinese)
Liu Y, Chen S, Chen X, Zhang J, Gao BY (2015a) Interactions between Microcystis aeruginosa and co-existing amoxicillin contaminant at different phosphorus levels. J Hazard Mater 297:83–91
Liu Y, Chen S, Zhang J, Gao BY (2015b) Nitrogen-regulated interactions between Microcystis aeruginosa and spiramycin contaminant. Water Air Soil Pollut 226:135
Liu Y, Chen X, Zhang J, Gao BY (2015c) Hormesis effects of amoxicillin on growth and cellular biosynthesis of Microcystis aeruginosa at different nitrogen levels. Microb Ecol 69:608–617
Liu Y, Wang F, Xiao C, Zhang J, Gao BY (2015d) Cellular responses and biodegradation of amoxicillin in Microcystis aeruginosa at different nitrogen levels. Ecotoxicol Environ Saf 111:138–145
Nie MH, Yan CX, Li M, Wang XN, Bi WL, Dong WB (2015) Degradation of chloramphenicol by persulfate activated by Fe2+ and zerovalent iron. Chem Eng J 279:507–515
Polyak Y, Zaytseva T, Medvedeva N (2013) Response of toxic cyanobacterium Microcystis aeruginosa to environmental pollution. Water Air Soil Pollut 224:1494
Thompson PA, Waite AM, McMahon K (2003) Dynamics of a cyanobacterial bloom in a hypereutrophic, stratified weir pool. Mar Freshw Res 54:27–37
Vézie C, Rapala J, Vaitomaa J, Seisonen J, Sivonen K (2002) Effect of nitrogen and phosphorus on growth of toxic and nontoxic Microcystis strains and on intracellular microcystin concentrations. Microb Ecol 43:443
Wang YJ, Li YW, Luo XY, Ren YA, Gao EG, Gao HJ (2018) Effects of yttrium and phosphorus on growth and physiological characteristics of Microcystis aeruginosa. J Rare Earth 36:781–788
Watanabe MM, Kaya K, Takamura N (1992) Fate of the toxic cyclic heptapeptides, the microcystins, from blooms of Microcystis (cyanobacteria) in a hypertrophic lake. J Phycol 28:761–767
Xu DM, Li CD, Chen H, Shao B (2013) Cellular response of freshwater green algae to perfluorooctanoic acid toxicity. Ecotoxicol Environ Saf 88:103–107
Zhang SH, Xu PY, Chang JJ (2016) Physiological responses of Aphanizomenon flos-aquae under the stress of Sagittaria sagittifolia extract. Bull Environ Contam Toxicol 97:870–875
Zurawell RW, Chen H, Burke JM, Prepas EE (2005) Hepatotoxic cyanobacteria: a review of the biological importance of microcystins in freshwater environments. J Toxicol Environ Health B 8:1–37
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This work was funded by the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130008120026).
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Luo, L., Li, J., Zhang, Z. et al. Phosphorus Influences the Interaction Between Toxigenic Microcystis and Chloramphenicol. Bull Environ Contam Toxicol 102, 391–398 (2019). https://doi.org/10.1007/s00128-018-2505-3
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DOI: https://doi.org/10.1007/s00128-018-2505-3