Abstract
Key message
A genetic linkage map representing proso millet genome was constructed with SSR markers, and a major QTL corresponding to plant height was mapped on chromosome 14 of this map.
Abstract
Proso millet (Panicum miliaceum L.) has the lowest water requirements of all cultivated cereal crops. However, the lack of a genetic map and the paucity of genomic resources for this species have limited the utility of proso millet for detailed genetic studies and hampered genetic improvement programs. In this study, 97,317 simple sequence repeat (SSR) markers were developed based on the genome sequence of the proso millet landrace Longmi 4. Using some of these markers in conjunction with previously identified SSRs, an SSR-based linkage map for proso millet was successfully constructed using a large mapping population (316 F2 offspring). In total, 186 SSR markers were assigned to 18 linkage groups corresponding to the haploid chromosomes. The constructed map had a total length of 3033.42 centimorgan (cM) covering 78.17% of the assembled reference genome. The length of the 18 linkage groups ranged from 88.89 cM (Chr. 15) to 274.82 cM (Chr. 16), with an average size of 168.17 cM. To our knowledge, this is the first genetic linkage map for proso millet based on SSR markers. Plant height is one of the most important traits in crop improvement. A major QTL was repeatedly detected in different environments, explaining 8.70–24.50% of the plant height variations. A candidate gene affecting auxin biosynthesis and transport, and ROS homeostasis regulation was predicted. Thus, the linkage map and QTL analysis provided herein will promote the development of gene mining and molecular breeding in proso millet.
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This work was funded by the National Natural Science Foundation of China (Grant Number: 32241043), the China Agricultural Research System (Grant Number: CARS-06-14.5-A10) and the HAAFS Basic Science and Technology Contract Project (Grant Number: HBNKY-BGZ-02-2021030202).
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GL conceptualized and supervised the study, acquired funding, and edited the original draft. YJ conducted the linkage map construction and wrote the original draft. LD generated the mapping populations and performed the QTL analysis. HL and YL collected the data in 2022. XW collected the data in 2018. All authors read and approved the manuscript.
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Jiang, Y., Dong, L., Li, H. et al. Genetic linkage map construction and QTL analysis for plant height in proso millet (Panicum miliaceum L.). Theor Appl Genet 137, 78 (2024). https://doi.org/10.1007/s00122-024-04576-2
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DOI: https://doi.org/10.1007/s00122-024-04576-2