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A major QTL co-localized on chromosome 6BL and its epistatic interaction for enhanced wheat stripe rust resistance

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Co-localization of a major QTL for wheat stripe rust resistance to a 3.9-cM interval on chromosome 6BL across both populations and another QTL on chromosome 2B with epistatic interaction.

Abstract

Cultivars with diverse resistance are the optimal strategy to minimize yield losses caused by wheat stripe rust (Puccinia striiformis f. sp. tritici). Two wheat populations involving resistant wheat lines P10078 and Snb“S” from CIMMYT were evaluated for stripe rust response in multiple environments. Pool analysis by Wheat660K SNP array showed that the overlapping interval on chromosome 6B likely harbored a major QTL between two populations. Then, linkage maps were constructed using KASP markers, and a co-localized locus with large effect on chromosome 6BL was detected using QTL analysis in both populations. The coincident QTL, named QYr.nwafu-6BL.2, explained 59.7% of the phenotypic maximum variation in the Mingxian 169 × P10078 and 52.5% in the Zhengmai 9023 × Snb“S” populations, respectively. This co-localization interval spanning 3.9 cM corresponds to ~ 30.5-Mb genomic region of the newest common wheat reference genome (IWGSC RefSeq v.1.0). In addition, another QTL was also detected on chromosome 2B in Zhengmai 9023 × Snb“S” population and it can accelerate expression of QYr.nwafu-6BL.2 to enhance resistance with epistatic interaction. Allowing for Pst response, marker genotypes, pedigree analysis and relative genetic distance, QYr.nwafu-6BL.2 is likely to be a distinct adult plant resistance QTL. Haplotype analysis of QYr.nwafu-6BL.2 revealed specific SNPs or alleles in the target region from a diversity panel of 176 unrelated wheat accessions. This QTL region provides opportunity for further map-based cloning, and haplotypes analysis enables pyramiding favorable alleles into commercial cultivars by marker-assisted selection.

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References

  • Abberton M, Batley J, Bentley A, Bryant J, Cai H, Cockram J, Costa De Oliveira A, Cseke LJ, Dempewolf H, De Pace C, Edwards D, Gepts P, Greenland A, Hall AE, Henry R, Hori K, Howe GT, Hughes S, Humphreys M, Lightfoot D, Marshall A, Mayes S, Nguyen HT, Ogbonnaya FC, Ortiz R, Paterson AH, Tuberosa R, Valliyodan B, Varshney RK, Yano M (2016) Global agricultural intensification during climate change: a role for genomics. Plant Biotechnol J 14:1095–1098

    Article  PubMed  Google Scholar 

  • Bai BB, Liu TG, Liu B, Gao L, Chen WQ (2017) High relative parasitic fitness of G22 derivatives is associated with the epidemic potential of wheat stripe rust in China. Plant Dis 102:483–487

    Article  PubMed  Google Scholar 

  • Bolouri Moghaddam MR, Van den Ende W (2012) Sugars and plant innate immunity. J Exp Bot 63:3989–3998

    Article  CAS  PubMed  Google Scholar 

  • Brummer EC, Barber WT, Collier SM, Cox TS, Johnson R, Murray SC, Olsen RT, Pratt RC, Thro AM (2011) Plant breeding for harmony between agriculture and the environment. Front Ecol Environ 9:561–568

    Article  Google Scholar 

  • Bulli P, Zhang J, Chao S, Chen X, Pumphrey M (2016) Genetic architecture of resistance to stripe rust in a global winter wheat germplasm collection. G3 6:2237–2253

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen XM (2013) High-temperature adult-plant resistance, key for sustainable control of stripe rust. Am J Plant Sci 04:608–627

    Article  Google Scholar 

  • Chen XM (2014) Integration of cultivar resistance and fungicide application for control of wheat stripe rust. Can J Plant Pathol 36:311–326

    Article  CAS  Google Scholar 

  • Chen XM, Kang ZS (2017) Stripe rust. Springer, Dordrecht, p 723

    Book  Google Scholar 

  • Chen WQ, Wu LR, Liu TG, Xu SC, Jin SL, Peng YL, Wang BT (2009) Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101

    Article  CAS  PubMed  Google Scholar 

  • CIMMYT (1983) Report on Wheat Improvement. International Maize and Wheat Improvement Center, Mexico, p 20

  • Cui F, Zhang N, Fan X, Zhang W, Zhao C, Yang L, Pan R, Chen M, Han J, Zhao X, Ji J, Tong Y, Zhang H, Jia J, Zhao G, Li J (2017) Utilization of a Wheat660K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number. Sci Rep 7:3788

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dedryver F, Paillard S, Mallard S, Robert O, Trottet M, Negre S, Verplancke G, Jahier J (2009) Characterization of genetic components involved in durable resistance to stripe rust in the bread wheat ‘Renan’. Phytopathology 99:968–973

    Article  CAS  PubMed  Google Scholar 

  • Doussinault G, Dosba F (1981) Analyse monosomique de la résistance a la rouille jaune du géniteur blé tendre VPM 1. Comptes rendus des seances de l’Academie d’agriculture de France, pp 133–138

  • Ellis JG, Lagudah ES, Spielmeyer W, Dodds PN (2014) The past, present and future of breeding rust resistant wheat. Front Plant Sci 5:1–13

    Article  Google Scholar 

  • Feng J, Chen G, Wei Y, Liu Y, Jiang Q, Li W, Pu Z, Lan X, Dai S, Zhang M, Zheng Y (2015) Identification and mapping stripe rust resistance gene YrLM168a using extreme individuals and recessive phenotype class in a complicate genetic background. Mol Genet Genom 290:2271–2278

    Article  CAS  Google Scholar 

  • Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res 19:6553–6568

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guo Q, Zhang ZJ, Xu YB, Li GH, Feng J, Zhou Y (2008) Quantitative trait loci for high-temperature adult-plant and slow-rusting resistance to Puccinia striiformis f. sp. tritici in wheat cultivars. Phytopathology 98:803–809

    Article  CAS  PubMed  Google Scholar 

  • Guzmán C, Autrique E, Mondal S, Huerta-Espino J, Singh RP, Vargas M, Crossa J, Amaya A, Peña RJ (2017) Genetic improvement of grain quality traits for CIMMYT semi-dwarf spring bread wheat varieties developed during 1965–2015: 50 years of breeding. Field Crop Res 210:192–196

    Article  Google Scholar 

  • Han D, Wang Q, Zhang L, Wei G, Zeng Q, Zhao J, Wang X, Huang L, Kang Z (2010) Evaluation of resistance of current wheat cultivars to stripe rust in Northwest China, North China and the Middle and Lower Reaches of Changjiang River epidemic area. Sci Agric Sin 43:2889–2896 (in Chinese with English summary)

    Google Scholar 

  • Han D, Zhang P, Wang Q, Zeng Q, Wu J, Zhou X, Wang X, Huang L, Kang Z (2012) Identification and evaluation of resistance to stripe rust in 1980 wheat landraces and abroad germplasm. Sci Agric Sin 45:5013–5023 (in Chinese with English summary)

    CAS  Google Scholar 

  • Han DJ, Wang QL, Chen XM, Zeng QD, Wu JH, Xue WB, Zhan GM, Huang LL, Kang ZS (2015) Emerging Yr26-virulent races of Puccinia striiformis f. sp. tritici are threatening wheat production in the Sichuan Basin, China. Plant Dis 99:754–760

    Article  CAS  PubMed  Google Scholar 

  • Hou L, Chen X, Wang M, See DR, Chao S, Bulli P, Jing J (2015) Mapping a large number of QTL for durable resistance to stripe rust in winter wheat Druchamp using SSR and SNP markers. PLoS ONE 10:e01267945

    Google Scholar 

  • Hovmøller MS (2007) Sources of seedling and adult plant resistance to Puccinia striiformis f. sp. tritici in European wheats. Plant Breed 126:225–233

    Article  CAS  Google Scholar 

  • Hovmøller MS, Walter S, Justesen AF (2010) Escalating threat of wheat rusts. Science 329:369

    Article  PubMed  Google Scholar 

  • IWGSC (2018) Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science 361:eaar7191

    Article  CAS  Google Scholar 

  • Jia J, Zhao G (2016) Wheat660 SNP array developed by CAAS. http://wheat.pw.usda.gov/ggpages/topics/Wheat660_SNP_array_developed_by_CAAS.pdf. Accessed 19 Feb 2018

  • Kosambi DD (1943) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Kwon SJ, Jin HC, Lee S, Nam MH, Chung JH, Kwon SI, Ryu C, Park OK (2009) GDSL lipase-like 1 regulates systemic resistance associated with ethylene signaling in Arabidopsis. Plant J 58:235–245

    Article  CAS  PubMed  Google Scholar 

  • Lan C, Liang S, Zhou X, Zhou G, Lu Q, Xia X, He Z (2010) Identification of genomic regions controlling adult-plant stripe rust resistance in Chinese landrace Pingyuan 50 through bulked segregant analysis. Phytopathology 100:313–318

    Article  PubMed  Google Scholar 

  • Li ZQ, Zeng SM (2002) Wheat rust in China. China Agriculture Press, Beijing

    Google Scholar 

  • Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America 1968–1987. US Department of Agriculture Technical Bulletin, p 74

  • Maccaferri M, Zhang J, Bulli P, Abate Z, Chao S, Cantu D, Bossolini E, Chen X, Pumphrey M, Dubcovsky J (2015) A genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a worldwide collection of hexaploid spring wheat (Triticum aestivum L.). G3 5:449–465

    Article  PubMed  PubMed Central  Google Scholar 

  • Macer RCF (1966) The formal and monosomic genetic analysis of stripe rust (Puccinia striiformis) resistance in wheat. Hereditas 2:127–142

    Google Scholar 

  • Mallard S, Gaudet D, Aldeia A, Abelard C, Besnard AL, Sourdille P, Dedryver F (2005) Genetic analysis of durable resistance to yellow rust in bread wheat. Theor Appl Genet 110:1401–1409

    Article  CAS  PubMed  Google Scholar 

  • McIntosh RA, Wellings CW, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, East Melbourne, pp 20–26

    Book  Google Scholar 

  • McIntosh R, Mu J, Han D, Kang Z (2018) Wheat stripe rust resistance gene Yr24/Yr26: a retrospective review. Crop J 6:321–329

    Article  Google Scholar 

  • Meng L, Li HH, Zhang LY, Wang JK (2015) QTL IciMapping: integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J 3:269–283

    Article  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Moore JW, Herrera-Foessel S, Lan C, Schnippenkoetter W, Ayliffe M, Huerta-Espino J, Lillemo M, Viccars L, Milne R, Periyannan S, Kong X, Spielmeyer W, Talbot M, Bariana H, Patrick JW, Dodds P, Singh R, Lagudah E (2015) A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nat Genet 47:1494–1498

    Article  CAS  PubMed  Google Scholar 

  • Naruoka Y, Ando K, Bulli P, Muleta KT, Rynearson S, Pumphrey MO (2016) Identification and validation of SNP markers linked to the stripe rust resistance gene in wheat. Crop Sci 56:3055

    Article  CAS  Google Scholar 

  • Periyannan S, Milne RJ, Figueroa M, Lagudah ES, Dodds PN (2017) An overview of genetic rust resistance: from broad to specific mechanisms. PLoS Pathog 13:e1006380

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res Sect C26:496–500

    Article  Google Scholar 

  • Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD, Akhunov ED, Dvořák J, Linkiewicz AM, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis CE, Greene RA, Kantety R, La Rota CM, Munkvold JD, Sorrells SF, Sorrells ME, Dilbirligi M, Sidhu D, Erayman M, Randhawa HS, Sandhu D, Bondareva SN, Gill KS, Mahmoud AA, Ma XF, Miftahudin, Gustafson JP, Conley EJ, Nduati V, Gonzalez-Hernandez JL, Anderson JA, Peng JH, Lapitan NL, Hossain KG, Kalavacharla V, Kianian SF, Pathan MS, Zhang DS, Nguyen HT, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Gill BS (2004) A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168:701–712

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ramirez-Gonzalez RH, Uauy C, Caccamo M (2015) PolyMarker: a fast polyploid primer design pipeline. Bioinformatics 31:2038–2039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rasheed A, Hao Y, Xia X, Khan A, Xu Y, Varshney RK, He Z (2017) Crop breeding chips and genotyping platforms: progress, challenges, and perspectives. Mol Plant 10:1047–1064

    Article  CAS  PubMed  Google Scholar 

  • Ray DK, Ramankutty N, Mueller ND, West PC, Foley JA (2012) Recent patterns of crop yield growth and stagnation. Nat Commun 3:1293

    Article  CAS  PubMed  Google Scholar 

  • Ren Y, He Z, Li J, Lillemo M, Wu L, Bai B, Lu Q, Zhu H, Zhou G, Du J, Lu Q, Xia X (2012) QTL mapping of adult-plant resistance to stripe rust in a population derived from common wheat cultivars Naxos and Shanghai 3/Catbird. Theor Appl Genet 125:1211–1221

    Article  PubMed  Google Scholar 

  • Rosewarne GM, Herrera-Foessel SA, Singh RP, Huerta-Espino J, Lan CX, He ZH (2013) Quantitative trait loci of stripe rust resistance in wheat. Theor Appl Genet 126:2427–2449

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schlötterer C, Tobler R, Kofler R, Nolte V (2014) Sequencing pools of individuals—mining genome-wide polymorphism data without big funding. Nat Rev Genet 15:749–763

    Article  CAS  PubMed  Google Scholar 

  • Sharma-Poudyal D, Chen XM, Wan AM, Zhan GM, Kang ZS, Cao SQ, Jin SL, Morgounov A, Akin B, Mert Z, Shah SJA, Bux H, Ashraf M, Sharma RC, Madariaga R, Puri KD, Wellings C, Xi KQ, Wanyera R, Manninger K, Ganzalez MI, Koyda M, Sanin S, Patzek LJ (2013) Virulence characterization of international collections of the wheat stripe rust pathogen, Puccinia striiformis f. sp. tritici. Plant Dis 97:379–386

    Article  CAS  PubMed  Google Scholar 

  • Song WN, Ko L, Henry RJ (1994) Polymorphisms in the α-amy1 gene of wild and cultivated barley revealed by the polymerase chain reaction. Theor Appl Genet 89:509–513

    Article  Google Scholar 

  • Stubbs RW (1985) Stripe rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, vol II. Academic Press, New York, pp 61–101

    Google Scholar 

  • Tang C, Xu Q, Zhao M, Wang X, Kang Z (2018) Understanding the lifestyles and pathogenicity mechanisms of obligate biotrophic fungi in wheat: the emerging genomics era. Crop J 6:60–67

    Article  Google Scholar 

  • Uauy C (2017) Wheat genomics comes of age. Curr Opin Plant Biol 36:142–148

    Article  PubMed  Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

  • Wan AM, Zhao ZH, Chen XM, He ZH, Jin SL, Jia QZ, Yao G, Yang JX, Wang BT, Li GB, Bi YQ, Yuan ZY (2004) Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Dis 88:896–904

    Article  PubMed  Google Scholar 

  • Wang JK (2009) Inclusive composite interval mapping of quantitative trait genes. Acta Agron Sin 35:239–245 (in Chinese with English summary)

    Article  CAS  Google Scholar 

  • Wang M, Wang S, Xia G (2015) From genome to gene: a new epoch for wheat research? Trends Plant Sci 20:380–387

    Article  CAS  PubMed  Google Scholar 

  • Wang M, Wang S, Liang Z, Shi W, Gao C, Xia G (2018) From genetic stock to genome editing: gene exploitation in wheat. Trends Biotechnol 36:160–172

    Article  CAS  PubMed  Google Scholar 

  • William HM, Singh RP, Huerta-Espino J, Palacios G, Suenaga K (2006) Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat. Genome 49:977–990

    Article  CAS  PubMed  Google Scholar 

  • Wu JH, Wang QL, Chen XM, Wang MJ, Mu JM, Lv XN, Huang LL, Han DJ, Kang ZS (2016) Stripe rust resistance in wheat breeding lines developed for central Shaanxi, an overwintering region for Puccinia striiformis f. sp. tritici in China. Can J Plant Pathol 38:317–324

    Article  CAS  Google Scholar 

  • Wu J, Wang Q, Liu S, Huang S, Mu J, Zeng Q, Huang L, Han D, Kang Z (2017) Saturation mapping of a major effect QTL for stripe rust resistance on wheat chromosome 2B in cultivar Napo 63 using SNP genotyping arrays. Front Plant Sci 8:653

    Article  PubMed  PubMed Central  Google Scholar 

  • Wu J, Huang S, Zeng Q, Liu S, Wang Q, Mu J, Yu S, Han D, Kang Z (2018a) Comparative genome-wide mapping versus extreme pool-genotyping and development of diagnostic SNP markers linked to QTL for adult plant resistance to stripe rust in common wheat. Theor Appl Genet 131:1777–1792

    Article  CAS  PubMed  Google Scholar 

  • Wu J, Liu S, Wang Q, Zeng Q, Mu J, Huang S, Yu S, Han D, Kang Z (2018b) Rapid identification of an adult plant stripe rust resistance gene in hexaploid wheat by high-throughput SNP array genotyping of pooled extremes. Theor Appl Genet 131:43–58

    Article  CAS  PubMed  Google Scholar 

  • Wu J, Wang Q, Xu L, Chen X, Li B, Mu J, Zeng Q, Huang L, Han D, Kang Z (2018c) Combining single nucleotide polymorphism genotyping array with bulked segregant analysis to map a gene controlling adult plant resistance to stripe rust in wheat line 03031-1-5 H62. Phytopathology 108:103–113

    Article  CAS  PubMed  Google Scholar 

  • Wu J, Zeng Q, Wang Q, Liu S, Yu S, Mu J, Huang S, Sela H, Distelfeld A, Huang L, Han D, Kang Z (2018d) SNP-based pool genotyping and haplotype analysis accelerate fine-mapping of the wheat genomic region containing stripe rust resistance gene Yr26. Theor Appl Genet 131:1481–1496

    Article  CAS  PubMed  Google Scholar 

  • Xu Y, Crouch JH (2008) Marker-assisted selection in plant breeding: from publications to practice. Crop Sci 48:391

    Article  Google Scholar 

  • Xu LS, Wang MN, Cheng P, Kang ZS, Hulbert SH, Chen XM (2013) Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theor Appl Genet 126:523–533

    Article  CAS  PubMed  Google Scholar 

  • Xu Y, Li P, Zou C, Lu Y, Xie C, Zhang X, Prasanna BM, Olsen MS (2017) Enhancing genetic gain in the era of molecular breeding. J Exp Bot 68:2641–2666

    Article  CAS  PubMed  Google Scholar 

  • Xue W, Xu X, Mu J, Wang Q, Wu J, Huang L, Kang Z, Han D (2014) Evaluation of stripe rust resistance and genes in Chinese elite wheat varieties. J Triticeae Crops 34:1054–1060 (in Chinese with English summary)

    CAS  Google Scholar 

  • Zeng Q, Han D, Wang Q, Yuan F, Wu J, Zhang L, Wang X, Huang L, Chen X, Kang Z (2014) Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines. Euphytica 196:271–284

    Article  CAS  Google Scholar 

  • Zeng QD, Shen C, Yuan FP, Wang QL, Wu JH, Xue WB, Zhan GM, Yao S, Chen W, Huang LL, Han DJ, Kang ZS (2015) The resistance evaluation of the Yr genes to the main prevalent pathotypes of Puccinia striiformis f. sp. tritici in China. Acta Phytopathol Sin 45:641–650 (in Chinese with English summary)

    Google Scholar 

  • Zeng QD, Wu JH, Liu SJ, Chen XM, Yuan FP, Su PP, Wang QL, Huang S, Mu JM, Han DJ, Kang ZS (2018) Genome-wide mapping for stripe rust resistance loci in common wheat cultivar Qinnong 142. Plant Dis. https://doi.org/10.1094/pdis-05-18-0846-re

    Article  PubMed  Google Scholar 

  • Zou C, Wang P, Xu Y (2016) Bulked sample analysis in genetics, genomics and crop improvement. Plant Biotechnol J 14:1941–1955

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors are grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, for review of this manuscript. Dr. Jianhui Wu thanks Mr Yue Liu for participating in making the figures. This study was financially supported by the National Science Foundation for Young Scientists in China (Grant 31701421), the Genetically Modified Organisms Breeding Major Project (2016ZX08002001), and the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-3-1-11).

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  1. Qingdong Zeng, Jianhui Wu and Shengjie Liu have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Qingdong Zeng, Jianhui Wu, Shengjie Liu, Shuo Huang, Qilin Wang, Jingmei Mu, Shizhou Yu, Dejun Han & Zhensheng Kang

  2. College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Shengjie Liu, Shuo Huang, Jingmei Mu, Shizhou Yu & Dejun Han

  3. College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Zhensheng Kang

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Table S1

. Genotypic data and stripe rust responses assessed by infection type (IT) and disease severity (DS) on the Mingxian 169 × P10078 RIL population (Table A) and Zhengmai 9023 × Snb“S” F2:3 lines (Table B) recorded at indicated dates at Yangling and Jiangyou during 2016–2017 (XLSX 126 kb)

Table S2.

Number of SNPs per megabase in different chromosomes from RIL (Table A) and F2:3 (Table B) bulks. (XLSX 64 kb)

Table S3.

Kompetitive allele specific PCR (KASP) primers used to genotype individual F2 plants and RILs for mapping stripe rust resistance loci in P10078 and Snb“S” (XLSX 12 kb)

Table S4.

The physical positions of EST in different sets of chromosome 2B (Table A) and 6B (Table B) bins (XLSX 46 kb)

Table A in S5.

Numbers of Mingxian 169 × P10078 RILs for resistance locus QYr.nwafu-6BL.2, showing mean stripe rust severities (%), when the resistance QTL was absent or present. Table B in S5. Effects of different combinations on number of QTL in combination in the F2:3 lines in the Zhengmai 9023 × Snb“S” population based on mean DS and IT in four field experiments (XLSX 30 kb)

Table S6.

Phenotypes and alleles of SNP markers flanking QYr.nwafu-6BL.2 and QYrsnb.nwafu-2BL in 176 wheat accessions including parents, donors of reported Yr genes/QTL on 6BL, susceptible checks, cultivars and advanced breeding lines (XLSX 47 kb)

Table S7.

Gene models between flanking markers AX-109585549 and AX-110989911 (positions 593,965,904 and 624,478,659, respectively, on chromosome 6B according to the Chinese Spring IWGSC RefSeq v1.0) (XLSX 38 kb)

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Zeng, Q., Wu, J., Liu, S. et al. A major QTL co-localized on chromosome 6BL and its epistatic interaction for enhanced wheat stripe rust resistance. Theor Appl Genet 132, 1409–1424 (2019). https://doi.org/10.1007/s00122-019-03288-2

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