Skip to main content
SpringerLink
Log in

Characterisation and mapping of gene Lr73 conferring seedling resistance to Puccinia triticina in common wheat

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

A gene conferring seedling resistance to Puccinia triticina was mapped to chromosome 2BS in the wheat Morocco. The gene was shown to be distinct and was therefore designated Lr73.

Abstract

The wheat genotype Morocco, widely susceptible to isolates of Puccinia triticina, was resistant to an Australian isolate of this pathogen collected in 2004. Genetic studies established that the resistance in Morocco was also present the Australian wheat genotypes Avocet, Halberd, Harrier, Tincurrin and a selection of cultivar Warigal lacking the resistance gene Lr20. Genetic studies based on a cross with Halberd showed that the gene is dominant and located on chromosome 2BS (XwPt8760—4 cM—Lr73—1.4 cM—XwPt8235). The gene was genetically independent of the Lr13, Lr16 and Lr23 loci, also located on chromosome 2BS, indicating that it is distinct. The locus designation Lr73 was therefore assigned. On the basis of multi-pathotype tests, it is likely Lr73 is also present in the Australian wheat cultivars Clearfield STL, Federation (with Lr10), Gatcher (with Lr10 and Lr27+Lr31), Marombi (with Lr1 and Lr37), Pugsley (with Lr1 and Lr37), Spear (with Lr1), Stiletto and Tarsa (with Lr1). Gene Lr73 is unlikely to be of value in resistance breeding. However, recognising Lr73 is important to avoid its inadvertent selection in breeding programmes. Furthermore, the apparent rarity of avirulence for genes like Lr73, sometimes referred to as “fossil” resistance genes, makes them of interest in terms of the evolution of disease resistance in host plants and of virulence in the respective rust pathogens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Rathmell B, Vaughan P, Huttner E, Kilian A (2006) Diversity Arrays Technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420

    Article  CAS  PubMed  Google Scholar 

  • Ali I, Roelfs AP, Huerta-Espino J (1994) Inheritance of leaf rust resistance in wheat cultivars Morocco and Little Club. Plant Dis 78:383–384

    Article  Google Scholar 

  • Bansal UK, Hayden MJ, Venkata BP, Khanna R, Saini RG, Bariana HS (2008) Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in wheat. Theor Appl Genet 117:307–312

    Article  CAS  PubMed  Google Scholar 

  • Dadkhodaie NA, Singh D, Park RF (2011) Characterisation of resistance to leaf rust in an international bread wheat nursery. J Plant Pathol 93:627–641

    Google Scholar 

  • El-Bedewy R, Röbbelen G (1982) Chromosomal location and change of dominance of a gene for resistance against yellow rust, Puccinia striiformis West, in wheat. Triticum aestivum L. Zeitschrift für Pflanzenzüchtung 89:145–157

    Google Scholar 

  • Ghazvini H, Hiebert CW, Thomas JB, Fetch T (2013) Development of a multiple bulked segregant analysis (MBSA) method used to locate a new stem rust resistance gene (Sr54) in the winter wheat cultivar Norin 40. Theor Appl Genet 126:443–449

    Article  PubMed  Google Scholar 

  • Hanson WD (1959) Minimum family sizes for planning of genetic experiments. Agron J 51:711–715

    Article  Google Scholar 

  • Huerta-Espino J, Singh RP, German S, McCallum BD, Park RF, Chen W, Bhardwaj SC, Goyeau H (2011) Global status of wheat leaf rust caused by Puccinia triticina. Euphytica. doi:10.1007/s10681-011-0361-x

    Google Scholar 

  • Knott DR, Anderson RG (1956) The inheritance of rust resistance. I. The inheritance of stem rust resistance in ten varieties of common wheat. Can J Agr Sci 36:174–195

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Annals of Eugenics 12:172–175

    Article  Google Scholar 

  • Loladze A (2011) Pathogen and host variability studies in wheat stripe rust in Australia. Sydney, Australia: University of Sydney, PhD thesis

  • McCartney CA, Somers DJ, McCallum BD, Thomas J, Humphreys DG, Menzies JG, Brown PD (2005) Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BS. Molec Breed 15:329–337

    Article  CAS  Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publishing, Melbourne

    Book  Google Scholar 

  • Nazari K, Wellings CR, Mohler V, Ahmed NU, Howes N (2005) Construction of a genetic linkage map for Cappelle Desprez/Avocet S doubled haploid population based on high-throughput DArT markers. In: Park RF, Bariana H, Wellings CR, (eds) “Global landscapes in cereal rust control—An international forum to mark the contribution of Prof R. A. McIntosh to the genetics of cereal rust control”. Katoomba, Australia, 57

  • Park RF, Fetch T, Hodson D, Jin Y, Nazari K, Prashar M, Pretorius ZA (2011) International surveillance of wheat rust pathogens—progress and challenges. Euphytica. doi:10.1007/s10681-011-0375-4

    Google Scholar 

  • Singh D, Mohler V, Park RF (2013) Discovery, characterization and mapping of wheat leaf rust resistance gene Lr71. Euphytica 190:131–136

    Article  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards KJ (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) Joinmap 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The Netherlands

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

  • Wellings CR, Bariana HS, Park RF, Bansal U (2011). Responses of Australian wheat and triticale varieties to the cereal rust diseases. Cereal Rust Report 9 (1): http://sydney.edu.au/agriculture/documents/pbi/cereal_rust_report_2011_vol_9_1.pdf

  • Xu LS, Wang MN, Cheng P, Kang ZS, Hulbert SH, Chen XM (2013) Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theor Appl Genet 126:523–533

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Financial support was provided by the Grains Research and Development Corporation. Technical assistance provided by Dr James Hull and Mr Matthew Williams is gratefully acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments reported in this manuscript comply with the current laws of Australia.

Author information

Authors and Affiliations

  1. The University of Sydney, Faculty of Agriculture and Environment, Plant Breeding Institute, Private Bag 4011, 2567, Narellan, NSW, Australia

    R. F. Park & D. Singh

  2. Bayerische Landesanstalt für Landwirtschaft (LfL), Institut für Pflanzenbau und Pflanzenzüchtung, Am Gereuth 6, 85354, Freising, Germany

    V. Mohler

  3. ICARDA, P.O. Box 5466, Aleppo, Syria

    K. Nazari

Authors
  1. R. F. Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Mohler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Nazari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. F. Park.

Additional information

Communicated by Beat Keller.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Park, R.F., Mohler, V., Nazari, K. et al. Characterisation and mapping of gene Lr73 conferring seedling resistance to Puccinia triticina in common wheat. Theor Appl Genet 127, 2041–2049 (2014). https://doi.org/10.1007/s00122-014-2359-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-014-2359-y

Keywords

Search

Navigation