Abstract
Leaf rust, caused by Puccinia triticina, is one of the most damaging diseases of wheat worldwide. Lr16 is a widely deployed leaf rust resistance gene effective at the seedling stage. Although virulence to Lr16 exists in the Canadian P. triticina population, Lr16 provides a level of partial resistance in the field. The primary objective of this study was to identify markers linked to Lr16 that are suitable for marker-assisted selection (MAS). Lr16 was tagged with microsatellite markers on the distal end of chromosome 2BS in three mapping populations. Seven microsatellite loci mapped within 10 cM of Lr16, with the map distances varying among populations. Xwmc764 was the closest microsatellite locus to Lr16, and mapped 1, 9, and 3 cM away in the RL4452/AC Domain, BW278/AC Foremost, and HY644/McKenzie mapping populations, respectively. Lr16 was the terminal locus mapped in all three populations. Xwmc764, Xgwm210, and Xwmc661 were the most suitable markers for selection of Lr16 because they had simple PCR profiles, numerous alleles, high polymorphism information content (PIC), and were tightly linked to Lr16. Twenty-eight spring wheat lines were evaluated for leaf rust reaction with the P. triticina virulence phenotypes MBDS, MBRJ, and MGBJ, and analyzed with five microsatellite markers tightly linked to Lr16. There was good agreement between leaf rust infection type (IT) data and the microsatellite allele data. Microsatellite markers were useful for postulating Lr16 in wheat lines with multiple leaf rust resistance genes.
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Y. Anikster W.R. Bushnell T. Eilam J. Manisterski A.P. Roelfs (1997) ArticleTitlePuccinia recondita causing leaf rust on cultivated wheats, wild wheats, and rye Can. J. Bot. 75 2082–2096
D. Botstein R.L. White M. Skolnick R.W. Davis (1980) ArticleTitleConstruction of a genetic linkage map in man using restriction fragment length polymorphisms Am. J. Hum. Genet. 32 314–331 Occurrence Handle1:CAS:528:DyaL3cXkvFamtbc%3D Occurrence Handle6247908
L.E. Browder (1973) ArticleTitleProbable genotype of some Triticum aestivum agent derivatives for reaction to Puccinia recondita f. sp. tritici Crop. Sci. 13 203–206
P.L. Dyck E.R. Kerber (1971) ArticleTitleChromosome location of three genes for leaf rust resistance in common wheat Can. J. Genet. Cytol. 13 480–483
G. Fedak M. Burvill H. Voldeng (1997) ArticleTitleA comparison of anther culture and maize pollination for haploid production in wheat J. Appl. Genet. 38 407–414
S.E. German J.A. Kolmer (1992) ArticleTitleEffect of gene Lr34 in the enhancement of resistance to leaf rust of wheat Theor. Appl. Genet. 84 97–105 Occurrence Handle10.1007/BF00223987 Occurrence Handle1:CAS:528:DyaK38XmtlShtLY%3D
L. Huang S.A. Brooks W. Li J.P. Fellers H.N. Trick B.S. Gill (2003) ArticleTitleMap-based cloning of leaf rust resistance gene Lr21 from the large and polyploidy genome of bread wheat Genetics 164 655–664 Occurrence Handle1:CAS:528:DC%2BD3sXlvFaisLg%3D Occurrence Handle12807786
J.A. Kolmer (1989) ArticleTitleVirulence and race dynamics of Puccinia recondita f. sp. tritici in Canada during 19561987 Phytopathology 79 349–356
J.A. Kolmer (1996) ArticleTitleGenetics of resistance to wheat leaf rust Annu. Rev. Phytopathol. 34 435–455 Occurrence Handle10.1146/annurev.phyto.34.1.435 Occurrence Handle1:CAS:528:DyaK28XlsFOhsLc%3D Occurrence Handle15012551
J.A. Kolmer (2001) ArticleTitlePhysiological specialization of Puccinia triticina in Canada in 1998 Plant Dis. 85 155–158
J.A. Kolmer J.Q. Liu (2002) ArticleTitleInheritance of leaf rust resistance in the wheat cultivars AC Majestic, AC Splendorand AC Karma Can. J. Plant Pathol. 24 327–331
J.Q. Liu J.A. Kolmer (1997a) ArticleTitleInheritance of leaf rust resistance in wheat cultivars Grandin and CDC Teal Plant Dis. 81 505–508
J.Q. Liu J.A. Kolmer (1997b) ArticleTitleGenetics of leaf rust resistance in Canadian spring wheats AC Domain and AC Taber Plant Dis. 81 757–760
W.Q. Loegering R.A. McIntosh C.H. Burton (1971) ArticleTitleComputer analysis of disease data to derive hypothetical genotypes for reaction of host varieties to pathogens Can. J. Genet. Cytol. 13 742–748
D.L. Long J.A. Kolmer (1989) ArticleTitleA North American System of Nomenclature for Puccinia recondita f. sp. tritici Phytopathology 79 525–529
B.D. McCallum P. Seto-Goh (2003) ArticleTitlePhysiological specialization of wheat leaf rust [Puccinia triticina] in Canada in 2000 Can. J. Plant Pathol. 25 91–97
B.D. McCallum P. Seto-Goh (2004) ArticleTitlePhysiological specialization of Puccinia triticinathe cause of wheat leaf rustin Canada in 2001 Can. J. Plant Pathol. 26 109–120
R.A. McIntosh N.H. Luig (1973) ArticleTitleLinkage of genes for reaction to Puccinia graminis f. sp. triticiP. recondita in Selkirk wheat and related cultivars Aust. J. Biol. Sci. 26 1145–1152
R.A. McIntosh C.R. Wellings R.F. Park (1995) Wheat Rusts: An Atlas of Resistance Genes Kluwer Academic Publishers DordrechtThe Netherlands 200
McIntosh R.A., Hart G.E., Devos K.M., Gale M.D. and Rogers W.J. 1998. Catalogue of Gene Symbols for Wheat. Proc. 9th Int. Wheat Genetics Symposium27 August 1998 Saskatoon, Saskatchewan, Canada.
D.V. McVey D.L. Long (1993) ArticleTitleGenes for leaf rust resistance in hard red winter wheat cultivars and parental lines Crop Sci. 33 1373–1381
M.S. Röder V. Korzun K. Wendehake J. Plaschke M.H. Tixier P. Leroy M.W. Ganal (1998) ArticleTitleA microsatellite map of wheat Genetics 149 2007–2023 Occurrence Handle9691054
D.J. Samborski (1985) Wheat leaf rust A.P. Roelfs W.R. Bushnell (Eds) The Cereal Rusts Academic Press New York 39–59
D.J. Samborski P.L. Dyck (1968) ArticleTitleInheritance of virulence in wheat leaf rust on standard differential varieties Can. J. Genet. Cytol. 10 24–32
D.J. Samborski P.L. Dyck (1982) ArticleTitleEnhancement of resistance to Puccinia recondita by interactions of resistance genes in wheat Can. J. Plant Pathol. 4 152–156
J.F. Schafer D.L. Long (1988) ArticleTitleRelations of races and virulences of Puccinia recondita f. sp. tritici to wheat cultivars and areas Plant Dis. 72 25–27
M. Schuelke (2000) ArticleTitleAn economic method for the fluorescent labeling of PCR fragments Nat. Biotechnol. 18 233–234 Occurrence Handle10.1038/72708 Occurrence Handle1:CAS:528:DC%2BD3cXhtVOksbk%3D Occurrence Handle10657137
R.P. Singh W.Q. Chen Z.H. He (1999) ArticleTitleLeaf rust resistance of spring, facultativeand winter wheat cultivars from China Plant Dis. 83 644–651
R.P. Singh S. Rajaram (1991) ArticleTitleResistance to Puccinia recondita f. sp. tritici in 50 Mexican bread wheat cultivars Crop Sci. 31 1472–1479
Somers D.J., Isaac P. and Edwards K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L).. Theor. Appl. Genet. eFIRST date: 29 July 2004.
P. Stam (1993) ArticleTitleConstruction of integrated genetic linkage maps by means of a new computer package: JoinMap Plant J. 3 739–744 Occurrence Handle1:CAS:528:DyaK3sXlvFKktLc%3D
M.W. Strickberger (1985) Genetics, 3rd ed Macmillan Publishing Company New York
L.E. Talbert N.K. Blake P.W. Chee T.K. Blake G.M. Magyar (1994) ArticleTitleEvaluation of ‘sequence-tagged-site’ PCR products as molecular markers in wheat Theor. Appl. Genet. 87 789–794 Occurrence Handle10.1007/BF00221130 Occurrence Handle1:CAS:528:DyaK2MXivV2hsA%3D%3D
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McCartney, C.A., Somers, D.J., McCallum, B.D. et al. Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BSc. Mol Breeding 15, 329–337 (2005). https://doi.org/10.1007/s11032-004-5948-7
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DOI: https://doi.org/10.1007/s11032-004-5948-7