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Effect of radiotherapy on the DNA cargo and cellular uptake mechanisms of extracellular vesicles

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Abstract

In the past decades, plenty of evidence has gathered pointing to the role of extracellular vesicles (EVs) secreted by irradiated cells in the development of radiation-induced non-targeted effects. EVs are complex natural structures composed of a phospholipid bilayer which are secreted by virtually all cells and carry bioactive molecules. They can travel certain distances in the body before being taken up by recipient cells. In this review we discuss the role and fate of EVs in tumor cells and highlight the importance of DNA specimens in EVs cargo in the context of radiotherapy. The effect of EVs depends on their cargo, which reflects physiological and pathological conditions of donor cell types, but also depends on the mode of EV uptake and mechanisms involved in the route of EV internalization. While the secretion and cargo of EVs from irradiated cells has been extensively studied in recent years, their uptake is much less understood. In this review, we will focus on recent knowledge regarding the EV uptake of cancer cells and the effect of radiation in this process.

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Funding

This work was supported by Euratom Research and Training Programme 2021–2025, grant agreement number 101061037.

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Authors and Affiliations

  1. Department of Radiobiology and Radiohygiene, Unit of Radiation Medicine, National Public Health Centre, 1097, Budapest, Hungary

    Tünde Szatmári, Katalin Balázs, Ilona Barbara Csordás, Géza Sáfrány & Katalin Lumniczky

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  1. Tünde Szatmári
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  2. Katalin Balázs
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  3. Ilona Barbara Csordás
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  4. Géza Sáfrány
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  5. Katalin Lumniczky
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Contributions

LK and TSz had the idea for the article; TSz, KB, and IBCs performed the literature search of different parts of the manuscript and drafted the work; GS and KL revised the manuscript critically. All authors approved the version to be published and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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Correspondence to Tünde Szatmári.

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Conflict of interest

T. Szatmári, K. Balázs, I.B. Csordás, G. Sáfrány, and K. Lumniczky declare that they have no competing interests.

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The authors Katalin Balázs and Ilona Barbara Csordás contributed equally to the manuscript.

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Szatmári, T., Balázs, K., Csordás, I.B. et al. Effect of radiotherapy on the DNA cargo and cellular uptake mechanisms of extracellular vesicles. Strahlenther Onkol 199, 1191–1213 (2023). https://doi.org/10.1007/s00066-023-02098-2

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