Abstract
Purkinje neurons, the sole output of the cerebellar cortex, deliver GABA-mediated inhibition to the deep cerebellar nuclei. To subserve this critical function, Purkinje neurons fire repetitively, and at high frequencies, features that have been linked to the unique properties of the voltage-gated sodium (Nav) channels expressed. In addition to the rapidly activating and inactivating, or transient, component of the Nav current (INaT) present in many types of central and peripheral neurons, Purkinje neurons, also expresses persistent (INaP) and resurgent (INaR) Nav currents. Considerable progress has been made in detailing the biophysical properties and identifying the molecular determinants of these discrete Nav current components, as well as defining their roles in the regulation of Purkinje neuron excitability. Here, we review this important work and highlight the remaining questions about the molecular mechanisms controlling the expression and the functioning of Nav currents in Purkinje neurons. We also discuss the impact of the dynamic regulation of Nav currents on the functioning of individual Purkinje neurons and cerebellar circuits.
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References
Llinas RR (1988) The intrinsic electrophysiological properties of mammalian neurons: insights into central nervous system function. Science 242(4886):1654–1664
Bean BP (2007) The action potential in mammalian central neurons. Nat Rev Neurosci 8(6):451–465. https://doi.org/10.1038/nrn2148
Thach WT (1968) Discharge of Purkinje and cerebellar nuclear neurons during rapidly alternating arm movements in the monkey. J Neurophysiol 31(5):785–797. https://doi.org/10.1152/jn.1968.31.5.785
Hausser M, Clark BA (1997) Tonic synaptic inhibition modulates neuronal output pattern and spatiotemporal synaptic integration. Neuron 19(3):665–678
Raman IM, Bean BP (1997) Resurgent sodium current and action potential formation in dissociated cerebellar Purkinje neurons. J Neurosci 17(12):4517–4526
Llinas R, Sugimori M (1980) Electrophysiological properties of in vitro Purkinje cell dendrites in mammalian cerebellar slices. J Physiol 305:197–213
Raman IM, Bean BP (1999) Ionic currents underlying spontaneous action potentials in isolated cerebellar Purkinje neurons. J Neurosci 19(5):1663–1674
Khaliq ZM, Gouwens NW, Raman IM (2003) The contribution of resurgent sodium current to high-frequency firing in Purkinje neurons: an experimental and modeling study. J Neurosci 23(12):4899–4912
Raman IM, Sprunger LK, Meisler MH, Bean BP (1997) Altered subthreshold sodium currents and disrupted firing patterns in Purkinje neurons of Scn8a mutant mice. Neuron 19(4):881–891
Meisler MH, Plummer NW, Burgess DL, Buchner DA, Sprunger LK (2004) Allelic mutations of the sodium channel SCN8A reveal multiple cellular and physiological functions. Genetica 122(1):37–45
Levin SI, Khaliq ZM, Aman TK, Grieco TM, Kearney JA, Raman IM, Meisler MH (2006) Impaired motor function in mice with cell-specific knockout of sodium channel Scn8a (NaV1.6) in cerebellar Purkinje neurons and granule cells. J Neurophysiol 96(2):785–793. https://doi.org/10.1152/jn.01193.2005
Kalume F, Yu FH, Westenbroek RE, Scheuer T, Catterall WA (2007) Reduced sodium current in Purkinje neurons from Nav1.1 mutant mice: implications for ataxia in severe myoclonic epilepsy in infancy. J Neurosci 27(41):11065–11074. https://doi.org/10.1523/JNEUROSCI.2162-07.2007
Bosch MK, Carrasquillo Y, Ransdell JL, Kanakamedala A, Ornitz DM, Nerbonne JM (2015) Intracellular FGF14 (iFGF14) is required for spontaneous and evoked firing in cerebellar Purkinje neurons and for motor coordination and balance. J Neurosci 35(17):6752–6769. https://doi.org/10.1523/JNEUROSCI.2663-14.2015
Eccles JC, Llinas R, Sasaki K (1966) The mossy fibre-granule cell relay of the cerebellum and its inhibitory control by Golgi cells. Exp Brain Res 1(1):82–101
Eccles JC, Llinas R, Sasaki K (1966) Parallel fibre stimulation and the responses induced thereby in the Purkinje cells of the cerebellum. Exp Brain Res 1(1):17–39
Eccles J, Llinas R, Sasaki K (1964) Excitation of cerebellar Purkinje cells by the climbing fibres. Nature 203:245–246
Eccles JC, Llinas R, Sasaki K (1966) The inhibitory interneurones within the cerebellar cortex. Exp Brain Res 1(1):1–16
Eccles J, Llinas R, Sasaki K (1964) Golgi cell inhibition in the cerebellar cortex. Nature 204:1265–1266
Eccles JC, Llinas R, Sasaki K (1966) The excitatory synaptic action of climbing fibres on the Purkinje cells of the cerebellum. J Physiol 182(2):268–296
Eccles JC, Llinas R, Sasaki K (1966) Intracellularly recorded responses of the cerebellar Purkinje cells. Exp Brain Res 1(2):161–183
Eccles JC, Llinas R, Sasaki K (1966) The action of antidromic impulses on the cerebellar Purkinje cells. J Physiol 182(2):316–345
Itō M (1984) The cerebellum and neural control, 1st edn. Raven Press, New York
Ito M, Yoshida M, Obata K (1964) Monosynaptic inhibition of the intracerebellar nuclei induced rom the cerebellar cortex. Experientia 20(10):575–576
Obata K, Ito M, Ochi R, Sato N (1967) Pharmacological properties of the postsynaptic inhibition by Purkinje cell axons and the action of gamma-aminobutyric acid on deiters neurones. Exp Brain Res 4(1):43–57
Obata K, Takeda K, Shinozaki H (1970) Further study on pharmacological properties of the cerebellar-induced inhibition of deiters neurones. Exp Brain Res 11(4):327–342
Andersen P, Eccles JC, Voorhoeve PE (1964) Postsynaptic inhibition of cerebellar Purkinje cells. J Neurophysiol 27:1138–1153. https://doi.org/10.1152/jn.1964.27.6.1138
McKay BE, Engbers JD, Mehaffey WH, Gordon GR, Molineux ML, Bains JS, Turner RW (2007) Climbing fiber discharge regulates cerebellar functions by controlling the intrinsic characteristics of Purkinje cell output. J Neurophysiol 97(4):2590–2604. https://doi.org/10.1152/jn.00627.2006
Bell CC, Grimm RJ (1969) Discharge properties of Purkinje cells recorded on single and double microelectrodes. J Neurophysiol 32(6):1044–1055
Heck DH, Thach WT, Keating JG (2007) On-beam synchrony in the cerebellum as the mechanism for the timing and coordination of movement. Proc Natl Acad Sci USA 104(18):7658–7663. https://doi.org/10.1073/pnas.0609966104
Person AL, Raman IM (2012) Purkinje neuron synchrony elicits time-locked spiking in the cerebellar nuclei. Nature 481(7382):502–505. https://doi.org/10.1038/nature10732
Welsh JP, Lang EJ, Suglhara I, Llinas R (1995) Dynamic organization of motor control within the olivocerebellar system. Nature 374(6521):453–457. https://doi.org/10.1038/374453a0
Lang EJ, Apps R, Bengtsson F, Cerminara NL, De Zeeuw CI, Ebner TJ, Heck DH, Jaeger D, Jorntell H, Kawato M, Otis TS, Ozyildirim O, Popa LS, Reeves AM, Schweighofer N, Sugihara I, Xiao J (2017) The roles of the olivocerebellar pathway in motor learning and motor control. A consensus paper. Cerebellum 16(1):230–252. https://doi.org/10.1007/s12311-016-0787-8
Llinas R, Sugimori M (1980) Electrophysiological properties of in vitro Purkinje cell somata in mammalian cerebellar slices. J Physiol 305:171–195
Raman IM, Bean BP (2001) Inactivation and recovery of sodium currents in cerebellar Purkinje neurons: evidence for two mechanisms. Biophys J 80(2):729–737. https://doi.org/10.1016/S0006-3495(01)76052-3
Jahnsen H, Llinas R (1984) Ionic basis for the electro-responsiveness and oscillatory properties of guinea-pig thalamic neurones in vitro. J Physiol 349:227–247
Huguenard JR (1996) Low-threshold calcium currents in central nervous system neurons. Annu Rev Physiol 58:329–348. https://doi.org/10.1146/annurev.ph.58.030196.001553
Pape HC (1996) Queer current and pacemaker: the hyperpolarization-activated cation current in neurons. Annu Rev Physiol 58:299–327. https://doi.org/10.1146/annurev.ph.58.030196.001503
Carter BC, Bean BP (2011) Incomplete inactivation and rapid recovery of voltage-dependent sodium channels during high-frequency firing in cerebellar Purkinje neurons. J Neurophysiol 105(2):860–871. https://doi.org/10.1152/jn.01056.2010
Gahwiler BH, Llano I (1989) Sodium and potassium conductances in somatic membranes of rat Purkinje cells from organotypic cerebellar cultures. J Physiol 417:105–122
Carter BC, Giessel AJ, Sabatini BL, Bean BP (2012) Transient sodium current at subthreshold voltages: activation by EPSP waveforms. Neuron 75(6):1081–1093. https://doi.org/10.1016/j.neuron.2012.08.033
Ransdell JL, Dranoff E, Lau B, Lo WL, Donermeyer DL, Allen PM, Nerbonne JM (2017) Loss of Navbeta4-mediated regulation of sodium currents in adult Purkinje neurons disrupts firing and impairs motor coordination and balance. Cell Rep 19(3):532–544. https://doi.org/10.1016/j.celrep.2017.03.068
Kuo CC, Bean BP (1994) Na+ channels must deactivate to recover from inactivation. Neuron 12(4):819–829
Lewis AH, Raman IM (2014) Resurgent current of voltage-gated Na(+) channels. J Physiol 592(22):4825–4838. https://doi.org/10.1113/jphysiol.2014.277582
Raman IM, Bean BP (1999) Properties of sodium currents and action potential firing in isolated cerebellar Purkinje neurons. Ann N Y Acad Sci 868:93–96
Carter BC, Bean BP (2009) Sodium entry during action potentials of mammalian neurons: incomplete inactivation and reduced metabolic efficiency in fast-spiking neurons. Neuron 64(6):898–909. https://doi.org/10.1016/j.neuron.2009.12.011
Prinz AA, Abbott LF, Marder E (2004) The dynamic clamp comes of age. Trends Neurosci 27(4):218–224. https://doi.org/10.1016/j.tins.2004.02.004
Sharp AA, O’Neil MB, Abbott LF, Marder E (1993) The dynamic clamp: artificial conductances in biological neurons. Trends Neurosci 16(10):389–394
Noda M, Shimizu S, Tanabe T, Takai T, Kayano T, Ikeda T, Takahashi H, Nakayama H, Kanaoka Y, Minamino N et al (1984) Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence. Nature 312(5990):121–127
Catterall WA (2010) Ion channel voltage sensors: structure, function, and pathophysiology. Neuron 67(6):915–928. https://doi.org/10.1016/j.neuron.2010.08.021
Bezanilla F (2000) The voltage sensor in voltage-dependent ion channels. Physiol Rev 80(2):555–592. https://doi.org/10.1152/physrev.2000.80.2.555
West JW, Patton DE, Scheuer T, Wang Y, Goldin AL, Catterall WA (1992) A cluster of hydrophobic amino acid residues required for fast Na(+)-channel inactivation. Proc Natl Acad Sci USA 89(22):10910–10914
Armstrong CM, Bezanilla F (1977) Inactivation of the sodium channel. II. Gating current experiments. J Gen Physiol 70(5):567–590
Bosmans F, Martin-Eauclaire MF, Swartz KJ (2008) Deconstructing voltage sensor function and pharmacology in sodium channels. Nature 456(7219):202–208. https://doi.org/10.1038/nature07473
Capes DL, Goldschen-Ohm MP, Arcisio-Miranda M, Bezanilla F, Chanda B (2013) Domain IV voltage-sensor movement is both sufficient and rate limiting for fast inactivation in sodium channels. J Gen Physiol 142(2):101–112. https://doi.org/10.1085/jgp.201310998
Yu FH, Catterall WA (2003) Overview of the voltage-gated sodium channel family. Genome Biol 4(3):207
Bezanilla F, Armstrong CM (1977) Inactivation of the sodium channel. I. Sodium current experiments. J Gen Physiol 70(5):549–566
Chanda B, Bezanilla F (2002) Tracking voltage-dependent conformational changes in skeletal muscle sodium channel during activation. J Gen Physiol 120(5):629–645
Horn R, Ding S, Gruber HJ (2000) Immobilizing the moving parts of voltage-gated ion channels. J Gen Physiol 116(3):461–476
Grieco TM, Afshari FS, Raman IM (2002) A role for phosphorylation in the maintenance of resurgent sodium current in cerebellar Purkinje neurons. J Neurosci 22(8):3100–3107
Chen C, Calhoun JD, Zhang Y, Lopez-Santiago L, Zhou N, Davis TH, Salzer JL, Isom LL (2012) Identification of the cysteine residue responsible for disulfide linkage of Na+ channel alpha and beta2 subunits. J Biol Chem 287(46):39061–39069. https://doi.org/10.1074/jbc.M112.397646
Yu FH, Westenbroek RE, Silos-Santiago I, McCormick KA, Lawson D, Ge P, Ferriera H, Lilly J, DiStefano PS, Catterall WA, Scheuer T, Curtis R (2003) Sodium channel beta4, a new disulfide-linked auxiliary subunit with similarity to beta2. J Neurosci 23(20):7577–7585
Lewis AH, Raman IM (2011) Cross-species conservation of open-channel block by Na channel beta4 peptides reveals structural features required for resurgent Na current. J Neurosci 31(32):11527–11536. https://doi.org/10.1523/JNEUROSCI.1428-11.2011
Grieco TM, Malhotra JD, Chen C, Isom LL, Raman IM (2005) Open-channel block by the cytoplasmic tail of sodium channel beta4 as a mechanism for resurgent sodium current. Neuron 45(2):233–244. https://doi.org/10.1016/j.neuron.2004.12.035
Chen Y, Yu FH, Sharp EM, Beacham D, Scheuer T, Catterall WA (2008) Functional properties and differential neuromodulation of Na(v)1.6 channels. Mol Cell Neurosci 38(4):607–615. https://doi.org/10.1016/j.mcn.2008.05.009
Aman TK, Grieco-Calub TM, Chen C, Rusconi R, Slat EA, Isom LL, Raman IM (2009) Regulation of persistent Na current by interactions between beta subunits of voltage-gated Na channels. J Neurosci 29(7):2027–2042. https://doi.org/10.1523/JNEUROSCI.4531-08.2009
Theile JW, Jarecki BW, Piekarz AD, Cummins TR (2011) Nav1.7 mutations associated with paroxysmal extreme pain disorder, but not erythromelalgia, enhance Navbeta4 peptide-mediated resurgent sodium currents. J Physiol 589(Pt 3):597–608. https://doi.org/10.1113/jphysiol.2010.200915
Wang GK, Edrich T, Wang SY (2006) Time-dependent block and resurgent tail currents induced by mouse beta4(154–167) peptide in cardiac Na+ channels. J Gen Physiol 127(3):277–289. https://doi.org/10.1085/jgp.200509399
Lewis AH, Raman IM (2013) Interactions among DIV voltage-sensor movement, fast inactivation, and resurgent Na current induced by the NaVbeta4 open-channel blocking peptide. J Gen Physiol 142(3):191–206. https://doi.org/10.1085/jgp.201310984
Bant JS, Raman IM (2010) Control of transient, resurgent, and persistent current by open-channel block by Na channel beta4 in cultured cerebellar granule neurons. Proc Natl Acad Sci USA 107(27):12357–12362. https://doi.org/10.1073/pnas.1005633107
Barbosa C, Tan ZY, Wang R, Xie W, Strong JA, Patel RR, Vasko MR, Zhang JM, Cummins TR (2015) Navbeta4 regulates fast resurgent sodium currents and excitability in sensory neurons. Mol Pain 11:60. https://doi.org/10.1186/s12990-015-0063-9
Miyazaki H, Oyama F, Inoue R, Aosaki T, Abe T, Kiyonari H, Kino Y, Kurosawa M, Shimizu J, Ogiwara I, Yamakawa K, Koshimizu Y, Fujiyama F, Kaneko T, Shimizu H, Nagatomo K, Yamada K, Shimogori T, Hattori N, Miura M, Nukina N (2014) Singular localization of sodium channel beta4 subunit in unmyelinated fibres and its role in the striatum. Nat Commun 5:5525. https://doi.org/10.1038/ncomms6525
Cahalan MD (1975) Modification of sodium channel gating in frog myelinated nerve fibres by Centruroides sculpturatus scorpion venom. J Physiol 244(2):511–534
Schiavon E, Sacco T, Cassulini RR, Gurrola G, Tempia F, Possani LD, Wanke E (2006) Resurgent current and voltage sensor trapping enhanced activation by a beta-scorpion toxin solely in Nav1.6 channel. Significance in mice Purkinje neurons. J Biol Chem 281(29):20326–20337. https://doi.org/10.1074/jbc.M600565200
Schiavon E, Pedraza-Escalona M, Gurrola GB, Olamendi-Portugal T, Corzo G, Wanke E, Possani LD (2012) Negative-shift activation, current reduction and resurgent currents induced by beta-toxins from Centruroides scorpions in sodium channels. Toxicon 59(2):283–293. https://doi.org/10.1016/j.toxicon.2011.12.003
Vega-Saenz de Miera EC, Rudy B, Sugimori M, Llinas R (1997) Molecular characterization of the sodium channel subunits expressed in mammalian cerebellar Purkinje cells. Proc Natl Acad Sci USA 94(13):7059–7064
Schaller KL, Caldwell JH (2003) Expression and distribution of voltage-gated sodium channels in the cerebellum. Cerebellum 2(1):2–9. https://doi.org/10.1080/14734220309424
Jarnot M, Corbett AM (2006) Immunolocalization of NaV1.2 channel subtypes in rat and cat brain and spinal cord with high affinity antibodies. Brain Res 1107(1):1–12. https://doi.org/10.1016/j.brainres.2006.05.090
Felts PA, Yokoyama S, Dib-Hajj S, Black JA, Waxman SG (1997) Sodium channel alpha-subunit mRNAs I, II, III, NaG, Na6 and hNE (PN1): different expression patterns in developing rat nervous system. Brain Res Mol Brain Res 45(1):71–82
Vacher H, Mohapatra DP, Trimmer JS (2008) Localization and targeting of voltage-dependent ion channels in mammalian central neurons. Physiol Rev 88(4):1407–1447. https://doi.org/10.1152/physrev.00002.2008
Lorincz A, Nusser Z (2008) Cell-type-dependent molecular composition of the axon initial segment. J Neurosci 28(53):14329–14340. https://doi.org/10.1523/JNEUROSCI.4833-08.2008
Xiao M, Bosch MK, Nerbonne JM, Ornitz DM (2013) FGF14 localization and organization of the axon initial segment. Mol Cell Neurosci 56:393–403. https://doi.org/10.1016/j.mcn.2013.07.008
Grieco TM, Raman IM (2004) Production of resurgent current in NaV1.6-null Purkinje neurons by slowing sodium channel inactivation with beta-pompilidotoxin. J Neurosci 24(1):35–42. https://doi.org/10.1523/JNEUROSCI.3807-03.2004
Khaliq ZM, Raman IM (2006) Relative contributions of axonal and somatic Na channels to action potential initiation in cerebellar Purkinje neurons. J Neurosci 26(7):1935–1944. https://doi.org/10.1523/JNEUROSCI.4664-05.2006
Palmer LM, Clark BA, Grundemann J, Roth A, Stuart GJ, Hausser M (2010) Initiation of simple and complex spikes in cerebellar Purkinje cells. J Physiol 588(Pt 10):1709–1717. https://doi.org/10.1113/jphysiol.2010.188300
Burgess DL, Kohrman DC, Galt J, Plummer NW, Jones JM, Spear B, Meisler MH (1995) Mutation of a new sodium channel gene, Scn8a, in the mouse mutant ‘motor endplate disease’. Nat Genet 10(4):461–465. https://doi.org/10.1038/ng0895-461
Kohrman DC, Plummer NW, Schuster T, Jones JM, Jang W, Burgess DL, Galt J, Spear BT, Meisler MH (1995) Insertional mutation of the motor endplate disease (med) locus on mouse chromosome 15. Genomics 26(2):171–177
Mantegazza M, Yu FH, Powell AJ, Clare JJ, Catterall WA, Scheuer T (2005) Molecular determinants for modulation of persistent sodium current by G-protein betagamma subunits. J Neurosci 25(13):3341–3349. https://doi.org/10.1523/JNEUROSCI.0104-05.2005
Meadows LS, Isom LL (2005) Sodium channels as macromolecular complexes: implications for inherited arrhythmia syndromes. Cardiovasc Res 67(3):448–458. https://doi.org/10.1016/j.cardiores.2005.04.003
Brackenbury WJ (2012) Voltage-gated sodium channels and metastatic disease. Channels (Austin) 6(5):352–361. https://doi.org/10.4161/chan.21910
Abriel H, Rougier JS, Jalife J (2015) Ion channel macromolecular complexes in cardiomyocytes: roles in sudden cardiac death. Circ Res 116(12):1971–1988. https://doi.org/10.1161/CIRCRESAHA.116.305017
Goldfarb M (2012) Voltage-gated sodium channel-associated proteins and alternative mechanisms of inactivation and block. Cell Mol Life Sci 69(7):1067–1076. https://doi.org/10.1007/s00018-011-0832-1
Patino GA, Isom LL (2010) Electrophysiology and beyond: multiple roles of Na+ channel beta subunits in development and disease. Neurosci Lett 486(2):53–59. https://doi.org/10.1016/j.neulet.2010.06.050
Brackenbury WJ, Calhoun JD, Chen C, Miyazaki H, Nukina N, Oyama F, Ranscht B, Isom LL (2010) Functional reciprocity between Na+ channel Nav1.6 and beta1 subunits in the coordinated regulation of excitability and neurite outgrowth. Proc Natl Acad Sci USA 107(5):2283–2288. https://doi.org/10.1073/pnas.0909434107
Yan H, Pablo JL, Wang C, Pitt GS (2014) FGF14 modulates resurgent sodium current in mouse cerebellar Purkinje neurons. Elife 3:e04193. https://doi.org/10.7554/eLife.04193
Shakkottai VG, Xiao M, Xu L, Wong M, Nerbonne JM, Ornitz DM, Yamada KA (2009) FGF14 regulates the intrinsic excitability of cerebellar Purkinje neurons. Neurobiol Dis 33(1):81–88. https://doi.org/10.1016/j.nbd.2008.09.019
van Swieten JC, Brusse E, de Graaf BM, Krieger E, van de Graaf R, de Koning I, Maat-Kievit A, Leegwater P, Dooijes D, Oostra BA, Heutink P (2003) A mutation in the fibroblast growth factor 14 gene is associated with autosomal dominant cerebellar ataxia [corrected]. Am J Hum Genet 72(1):191–199
Goldfarb M (2005) Fibroblast growth factor homologous factors: evolution, structure, and function. Cytokine Growth Factor Rev 16(2):215–220. https://doi.org/10.1016/j.cytogfr.2005.02.002
Olsen SK, Garbi M, Zampieri N, Eliseenkova AV, Ornitz DM, Goldfarb M, Mohammadi M (2003) Fibroblast growth factor (FGF) homologous factors share structural but not functional homology with FGFs. J Biol Chem 278(36):34226–34236. https://doi.org/10.1074/jbc.M303183200
Liu C, Dib-Hajj SD, Waxman SG (2001) Fibroblast growth factor homologous factor 1B binds to the C terminus of the tetrodotoxin-resistant sodium channel rNav1.9a (NaN). J Biol Chem 276(22):18925–18933. https://doi.org/10.1074/jbc.M101606200
Liu CJ, Dib-Hajj SD, Renganathan M, Cummins TR, Waxman SG (2003) Modulation of the cardiac sodium channel Nav1.5 by fibroblast growth factor homologous factor 1B. J Biol Chem 278(2):1029–1036. https://doi.org/10.1074/jbc.M207074200
Wittmack EK, Rush AM, Craner MJ, Goldfarb M, Waxman SG, Dib-Hajj SD (2004) Fibroblast growth factor homologous factor 2B: association with Nav1.6 and selective colocalization at nodes of Ranvier of dorsal root axons. J Neurosci 24(30):6765–6775. https://doi.org/10.1523/JNEUROSCI.1628-04.2004
Goldfarb M, Schoorlemmer J, Williams A, Diwakar S, Wang C, Huan X, Giza J, Tchetchik D, Kelley K, Vega A, Matthews G, Rossi P, Ornitz DM, D’Angelo E (2007) Fibroblast growth factor homologous factors control neuronal excitability through modulation of voltage-gated sodium channels. Neuron 55(3):449–463. https://doi.org/10.1016/j.neuron.2007.07.006
Wang Q, Bardgett ME, Wong M, Wozniak DF, Lou J, McNeil BD, Chen C, Nardi A, Reid DC, Yamada K, Ornitz DM (2002) Ataxia and paroxysmal dyskinesia in mice lacking axonally transported FGF14. Neuron 35(1):25–38
Kazen-Gillespie KA, Ragsdale DS, D’Andrea MR, Mattei LN, Rogers KE, Isom LL (2000) Cloning, localization, and functional expression of sodium channel beta1A subunits. J Biol Chem 275(2):1079–1088
Shah BS, Stevens EB, Pinnock RD, Dixon AK, Lee K (2001) Developmental expression of the novel voltage-gated sodium channel auxiliary subunit beta3, in rat CNS. J Physiol 534(Pt 3):763–776
Yan H, Wang C, Marx SO, Pitt GS (2017) Calmodulin limits pathogenic Na+ channel persistent current. J Gen Physiol 149(2):277–293. https://doi.org/10.1085/jgp.201611721
Ben-Johny M, Yang PS, Niu J, Yang W, Joshi-Mukherjee R, Yue DT (2014) Conservation of Ca2+/calmodulin regulation across Na and Ca2+ channels. Cell 157(7):1657–1670. https://doi.org/10.1016/j.cell.2014.04.035
Shavkunov AS, Wildburger NC, Nenov MN, James TF, Buzhdygan TP, Panova-Elektronova NI, Green TA, Veselenak RL, Bourne N, Laezza F (2013) The fibroblast growth factor 14.voltage-gated sodium channel complex is a new target of glycogen synthase kinase 3 (GSK3). J Biol Chem 288(27):19370–19385. https://doi.org/10.1074/jbc.M112.445924
Shavkunov A, Panova N, Prasai A, Veselenak R, Bourne N, Stoilova-McPhie S, Laezza F (2012) Bioluminescence methodology for the detection of protein–protein interactions within the voltage-gated sodium channel macromolecular complex. Assay Drug Dev Technol 10(2):148–160. https://doi.org/10.1089/adt.2011.413
Hsu WC, Scala F, Nenov MN, Wildburger NC, Elferink H, Singh AK, Chesson CB, Buzhdygan T, Sohail M, Shavkunov AS, Panova NI, Nilsson CL, Rudra JS, Lichti CF, Laezza F (2016) CK2 activity is required for the interaction of FGF14 with voltage-gated sodium channels and neuronal excitability. FASEB J 30(6):2171–2186. https://doi.org/10.1096/fj.201500161
Eom TY, Jope RS (2009) Blocked inhibitory serine-phosphorylation of glycogen synthase kinase-3alpha/beta impairs in vivo neural precursor cell proliferation. Biol Psychiatry 66(5):494–502. https://doi.org/10.1016/j.biopsych.2009.04.015
Doble BW, Patel S, Wood GA, Kockeritz LK, Woodgett JR (2007) Functional redundancy of GSK-3alpha and GSK-3beta in Wnt/beta-catenin signaling shown by using an allelic series of embryonic stem cell lines. Dev Cell 12(6):957–971. https://doi.org/10.1016/j.devcel.2007.04.001
Mao Y, Ge X, Frank CL, Madison JM, Koehler AN, Doud MK, Tassa C, Berry EM, Soda T, Singh KK, Biechele T, Petryshen TL, Moon RT, Haggarty SJ, Tsai LH (2009) Disrupted in schizophrenia 1 regulates neuronal progenitor proliferation via modulation of GSK3beta/beta-catenin signaling. Cell 136(6):1017–1031. https://doi.org/10.1016/j.cell.2008.12.044
Cuesto G, Jordan-Alvarez S, Enriquez-Barreto L, Ferrus A, Morales M, Acebes A (2015) GSK3beta inhibition promotes synaptogenesis in Drosophila and mammalian neurons. PLoS One 10(3):e0118475. https://doi.org/10.1371/journal.pone.0118475
Smillie KJ, Cousin MA (2011) The role of GSK3 in presynaptic function. Int J Alzheimers Dis 2011:263673. https://doi.org/10.4061/2011/263673
Peineau S, Taghibiglou C, Bradley C, Wong TP, Liu L, Lu J, Lo E, Wu D, Saule E, Bouschet T, Matthews P, Isaac JT, Bortolotto ZA, Wang YT, Collingridge GL (2007) LTP inhibits LTD in the hippocampus via regulation of GSK3beta. Neuron 53(5):703–717. https://doi.org/10.1016/j.neuron.2007.01.029
Hooper C, Markevich V, Plattner F, Killick R, Schofield E, Engel T, Hernandez F, Anderton B, Rosenblum K, Bliss T, Cooke SF, Avila J, Lucas JJ, Giese KP, Stephenson J, Lovestone S (2007) Glycogen synthase kinase-3 inhibition is integral to long-term potentiation. Eur J Neurosci 25(1):81–86. https://doi.org/10.1111/j.1460-9568.2006.05245.x
Acknowledgements
The authors thank Richard Wilson for technical assistance in creating figures. Financial support provided by the National Institutes of Health (R01NS065761 to J.M.N., F32NS090765 to J.L.R.) is also gratefully acknowledged.
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Ransdell, J.L., Nerbonne, J.M. Voltage-gated sodium currents in cerebellar Purkinje neurons: functional and molecular diversity. Cell. Mol. Life Sci. 75, 3495–3505 (2018). https://doi.org/10.1007/s00018-018-2868-y
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DOI: https://doi.org/10.1007/s00018-018-2868-y