Abstract
The rapid rise in CO2 emissions owing to fossil fuel combustion and increasing energy demands worldwide has fuelled the research for finding a viable substitute for fossil fuels. Microalgal biofuels, also known as 3G biofuels, hold the promise of being a sustainable yet economical substitute for conventional fossil fuels. The relatively untenable production of biofuels from food crops had its share of twofold complicit concerns regarding food security and economic viability. Microalgae on the other hand are compounded with benefits of CO2 sequestration and rapid biomass accumulation at low energy inputs, only to be restricted by the scale of biofuel recovery. Pretreatment strategies for microalgae have been the key driver for efficient microalgal biofuel production through mechanical, thermal, and chemical procedures. With the advent of pioneering approaches entailing enzymatic and microbial pretreatments alongside the conventional approaches, productivity has further increased. In this chapter, we summarize the recent developments in the enzymatic and biocatalytic pretreatment methods for improving the yield of valuable products from microalgae. The chapter also discusses the various factors affecting the pretreatment process and provides an understanding of the future perspectives for exploring newer prospects.
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Abbreviations
- BP:
-
biofuel production
- EP:
-
enzymatic pretreatment
- BAP:
-
bacterial pretreatment
- FP:
-
fungal pretreatment
- SRF:
-
soft-rot fungi
- WRF:
-
white-rot fungi
- BRF:
-
brown-rot fungi
- 1G:
-
first generation
- 2G:
-
second generation
- 3G:
-
third generation
- 4G:
-
fourth generation
References
Adsul M, Sandhu SK, Singhania RR, Gupta R, Puri SK, Mathur A (2020) Designing a cellulolytic enzyme cocktail for the efficient and economical conversion of lignocellulosic biomass to biofuels. Enzyme Microb Technol 133:109442
Agrawal K, Verma P (2020) Production optimization of yellow laccase from Stropharia sp. ITCC 8422 and enzyme-mediated depolymerization and hydrolysis of lignocellulosic biomass for biorefinery application. Biomass Conver Biorefinery 5:1–20
Alavijeh RS, Karimi K, Wijffels RH, van den Berg C, Eppink M (2020) Combined bead milling and enzymatic hydrolysis for efficient fractionation of lipids, proteins and carbohydrates of Chlorella vulgaris microalgae. Bioresour Technol 309:123321
Alexandropoulou M, Antonopoulou G, Fragkou E, Ntaikou I, Lyberatos G (2017) Fungal pretreatment of willow sawdust and its combination with alkaline treatment for enhancing biogas production. J Environ Manage 203:704–713
Ali SS, Abomohra AE-F, Sun J (2017) Effective bio-pretreatment of sawdust waste with a novel microbial consortium for enhanced biomethanation. Bioresour Technol 238:425–432
Ananthi V, Brindhadevi K, Pugazhendhi A, Arun A (2021) Impact of abiotic factors on biodiesel production by microalgae. Fuel 284:118962
Ananthi V, Pugazhendhi A, Arun A (2020) Efficacy of chemical factors on production and extraction of biodiesel by microalgae. Int J Energy Res 2020:1–14
Anwar M, Lou S, Chen L, Li H, Hu Z (2019) Recent advancement and strategy on bio-hydrogen production from photosynthetic microalgae. Bioresour Technol 292:121972
Aydin S, Yıldırım E, Ince O, Ince B (2017) Rumen anaerobic fungi create new opportunities for enhanced methane production from microalgae biomass. Algal Res 23:150–160
Bai M-D, Chen C-Y, Lu W-C, Wan H-P, Ho S-H, Chang J-S (2015) Enhancing the oil extraction efficiency of Chlorella vulgaris with cell-disruptive pretreatment using active extracellular substances from bacillus thuringiensis ITRI-G1. Biochem Eng J 101:185–190
Barati B, Zafar FF, Rupani PF, Wang S (2021) Bacterial pretreatment of microalgae and the potential of novel nature hydrolytic sources. Environ Technol Innov 21:101362
Barragán-Trinidad M, Carrillo-Reyes J, Buitrón G (2017) Hydrolysis of microalgal biomass using ruminal microorganisms as a pretreatment to increase methane recovery. Bioresour Technol 244(1):100–107
Bhardwaj N, Agrawal K, Verma P (2020) Algal biofuels: an economic and effective alternative of fossil fuels. In: Srivastava N, Srivastava M, Mishra PK, Gupta VK (eds) Microbial strategies for techno-economic biofuel production. Springer, Singapore, pp 207–227
Bhushan S, Rana MS, Bhandari M, Sharma AK, Simsek H, Prajapati SK (2021) Enzymatic pretreatment of algal biomass has different optimal conditions for biogas and bioethanol routes. Chemosphere 284:131264
Çakmak EK, Ugurlu A (2020) Enhanced biogas production of red microalgae via enzymatic pretreatment and preliminary economic assessment. Algal Res 50:101979
Carmen M, Oana NN, Andreea-Daniela D, Eduard-Marius L (2019) Enzymatic pretreatment of algal biomass for enhanced conversion to biogas. J Eng Sci Innov 4(4):361–370
Carrillo-Reyes J, Barragán-Trinidad M, Buitrón G (2016) Biological pretreatments of microalgal biomass for gaseous biofuel production and the potential use of rumen microorganisms: a review. Algal Res 18:341–351
Cavinato C, Ugurlu A, de Godos I, Kendir E, Gonzalez-Fernandez C (2017) Biogas production from microalgae. In: Gonzalez-Fernandez C, Muñoz R (eds) Microalgae-based biofuels and bioproducts from feedstock cultivation to end-products. Woodhead Publishing, New York, pp 155–182
Córdova O, Passos F, Chamy R (2019) Enzymatic pretreatment of microalgae: cell wall disruption, biomass solubilisation and methane yield increase. Appl Biochem Biotechnol 189:787–797
Córdova O, Santis J, Ruiz-Fillipi G, Zuñiga ME, Fermoso FG, Chamy R (2018) Microalgae digestive pretreatment for increasing biogas production. Renew Sustain Energy Rev 82:2806–2813
Costa JAV, Freitas BCB, Moraes L, Zaparoli M, Morais MG (2020) Progress in the physicochemical treatment of microalgae biomass for value-added product recovery. Bioresour Technol 301:122727
Dalena F, Senatore A, Basile M, Marino D, Basile A (2019) From sugars to ethanol-from agricultural wastes to algal sources. In: Basile A, Dalena F (eds) Second and third generation of feedstocks the evolution of biofuels. Elsevier, Amsterdam, pp 557–597
Dar RA, Phutela UG (2020) Enzymatic and hydrothermal pretreatment of newly isolated Spirulina subsalsa BGLR6 biomass for enhanced biogas production. Waste Biomass Valoriz 11:3639–3651
Debnath C, Bandyopadhyay TK, Bhunia B, Mishra U, Narayanasamy S, Muthuraj M (2021) Microalgae: sustainable resource of carbohydrates in third-generation biofuel production. Renew Sustain Energy Rev 150:111464
Fang X, Li Q, Lin Y, Lin X, Dai Y, Guo Z, Pan D (2018) Screening of a microbial consortium for selective degradation of lignin from tree trimmings. Bioresour Technol 254:247–255
Ferdeș M, Dincă MN, Moiceanu G, Zăbavă BS, Paraschiv G (2020) Microorganisms and enzymes used in the biological pretreatment of the substrate to enhance biogas production: a review. Sustainability 12(17):7205
Godbole V, Pal MK, Gautam P (2021) A critical perspective on the scope of interdisciplinary approaches used in fourth-generation biofuel production. Algal Res 58:102436
Goswami RK, Mehariya S, Verma P, Lavecchia R, Zuorro A (2020a) Microalgae-based biorefineries for sustainable resource recovery from wastewater. J Water Process Engin 40:101747
Goswami RK, Agrawal K, Mehariya S, Molino A, Musmarra D, Verma P (2020b) Microalgae-based biorefinery for utilization of carbon dioxide for production of valuable bioproducts. In: Kumar A, Sharma S (eds) Chemo-biological systems for CO2 utilization. CRC Press, New York, pp 203–228
He S, Fan X, Katukuri NR, Yuan X, Wang F, Guo R-B (2016) Enhanced methane production from microalgal biomass by anaerobic bio-pretreatment. Bioresour Technol 204:145–151
He Y, Wu T, Wang X, Chen B, Chen F (2018) Cost-effective biodiesel production from wet microalgal biomass by a novel two-step enzymatic process. Bioresour Technol 268:583–591
Hom-Diaz A, Passos F, Ferrer I, Vicent T, Blánquez P (2016) Enzymatic pretreatment of microalgae using fungal broth from Trametes versicolor and commercial laccase for improved biogas production. Algal Res 19:184–188
Hu J, Xue Y, Guo H, Gao M-t, Li J, Zhang S, Tsang YF (2017) Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity. Bioresour Technol 227:247–255
Kadir WNA, Lam MK, Uemura Y, Lim JW, Lee KT (2018) Harvesting and pretreatment of microalgae cultivated in wastewater for biodiesel production: a review. Energ Conver Manage 171:1416–1429
Kannah RY, Kavitha S, Karthikeyan OP, Rene ER, Kumar G, Banu JR (2021) A review on anaerobic digestion of energy and cost effective microalgae pretreatment for biogas production. Bioresour Technol 332:125055
Karim A, Islam MA, Khalid ZB, Faizal CKM, Khan MDMR, Yousuf A (2020) Microalgal cell disruption and lipid extraction techniques for potential biofuel production. In: Yousuf A (ed) Microalgae cultivation for biofuels production. Elsevier, Amsterdam, pp 129–147
Kavitha S, Kannah RY, Banu JR, Kaliappan S, Johnson M (2017) Biological disintegration of microalgae for biomethane recovery-prediction of biodegradability and computation of energy balance. Bioresour Technol 224(2):1367–1375
Kendir E, Ugurlu A (2018) A comprehensive review on pretreatment of microalgae for biogas production. Int J Energy Res 42:3711–3731
Lin C-Y, Lu C (2021) Development perspectives of promising lignocellulose feedstocks for production of advanced generation biofuels: a review. Renew Sustain Energy Rev 136:110445
Liu Y, Cruz-Morales P, Zargar A, Belcher MS, Pang B, Englund E, Dan Q, Yin K, Keasling JD (2021) Biofuels for a sustainable future. Cell 184:1636–1647
Maffei G, Bracciale MP, Broggi A, Zuorro A, Santarelli ML, Lavecchia R (2018) Effect of an enzymatic treatment with cellulase and mannanase on the structural properties of Nannochloropsis microalgae. Bioresour Technol 249:592–598
Magdalena JA, Ballesteros M, González-Fernandez C (2018) Efficient anaerobic digestion of microalgae biomass: proteins as a key macromolecule. Molecules 23:1098
Maurya DP, Singla A, Negi S (2015) An overview of key pretreatment processes for biological conversion of lignocellulosic biomass to bioethanol. 3 Biotech 5:597–609
Meehnian H, Jana AK, Jana MM (2016) Effect of particle size, moisture content, and supplements on selective pretreatment of cotton stalks by Daedalea flavida and enzymatic saccharification. 3 Biotech 6:235
Mehariya S, Goswami RK, Verma P, Lavecchia R, Zuorro A (2021a) Integrated approach for wastewater treatment and biofuel production in microalgae biorefineries. Energies 14:2282
Mehariya S, Goswami RK, Karthikeysan OP, Verma P (2021b) Microalgae for high-value products: a way towards green nutraceutical and pharmaceutical compounds. Chemosphere 280:130553
Moodley P (2021) 1- sustainable biofuels: opportunities and challenges. Appl Biotechnol Rev 2021:1–20
Muñoz C, Hidalgo C, Zapata M, Jeison D, Riquelme C, Rivas M (2014) Use of cellulolytic marine bacteria for enzymatic pretreatment in microalgal biogas production. Appl Environ Microbiol 80(14):4199–4206
Mustafa AM, Poulsen TG, Sheng K (2016) Fungal pretreatment of rice straw with Pleurotus ostreatus and Trichoderma reesei to enhance methane production under solid-state anaerobic digestion. Appl Energy 180:661–671
Mustafa AM, Poulsen TG, Xia Y, Sheng K (2017) Combinations of fungal and milling pretreatments for enhancing rice straw biogas production during solid-state anaerobic digestion. Bioresour Technol 224:174–182
Nagappan S, Devendran S, Tsai P-C, Dahms H-U, Ponnusamy VK (2019) Potential of two-stage cultivation in microalgae biofuel production. Fuel 252:339–349
Nagarajan D, Changa J-S, Lee D-J (2020) Pretreatment of microalgal biomass for efficient biohydrogen production – recent insights and future perspectives. Bioresour Technol 2020:1–14
Onumaegbua C, Mooneya J, Alaswadb A, Olabi AG (2018) Pretreatment methods for production of biofuel from microalgae biomass. Renew Sustain Energy Rev 93:16–26
Padmaperuma G, Kapoore RV, Gilmour DJ, Vaidyanathan S (2018) Microbial consortia: a critical look at microalgae co-cultures for enhanced biomanufacturing. Crit Rev Biotechnol 38:690–703
Passos F, Hom-Diaz A, Blanquez P, Vicent T, Ferrer I (2016) Improving biogas production from microalgae by enzymatic pretreatment. Bioresour Technol 199:347–351
Paul V, Rai S, Tripathi AD, Rai DC, Agarwal A (2021) Impact of fermentation types on enzymes used for biofuels production. In: Srivastava N, Srivastava M, Mishra PK, Gupta VK (eds) Bioprocessing for biofuel production strategies to improve process parameters. Springer, Singapore, pp 1–27
Raven S, Ekka SB, Chattree SE, Sadanand SS, Rina L, Tiwari A (2021) Microbial technology for biofuel production. In: Srivastava N, Srivastava M (eds) Bioenergy research: evaluating strategies for commercialization and sustainability. Wiley, New York, pp 19–45
Rodriguez C, Alaswad A, Benyounis KY, Olabi AG (2017) Pretreatment techniques used in biogas production from grass. Renew Sustain Energy Rev 68:1193–1204
Rouches E, Herpoël-Gimbert I, Steyer JP, Carrere H (2016) Improvement of anaerobic degradation by white-rot fungi pretreatment of lignocellulosic biomass: a review. Renew Sustain Energy Rev 59:179–198
Sadvakasova AK, Kossalbayev BD, Zayadan BK, Kirbayeva DK, Alwasel S, Allakhverdiev SI (2021) Potential of cyanobacteria in the conversion of wastewater to biofuels. World J Microbiol Biotechnol 37:140
Saha BC, Kennedy GJ, Qureshi N, Cotta MA (2017) Biological pretreatment of corn Stover with Phlebia brevispora NRRL-13108 for enhanced enzymatic hydrolysis and efficient ethanol production. Biotechnol Prog 33:365–374
Sangwijit K, Jitonnom J, Pitakrattananukool S, Yu LD, Anuntalabhochai S (2016) Low-energy plasma immersion ion implantation modification of bacteria to enhance hydrolysis of biomass materials. Surf Coat Technol 306:336–340
Sankaran R, Cruz RAP, Pakalapati H, Show PL, Ling TC, Chen WH, Tao Y (2020) Recent advances in the pretreatment of microalgal and lignocellulosic biomass: a comprehensive review. Bioresour Technol 298:122476
Sharma HK, Xu C, Qin W (2019) Biological pretreatment of lignocellulosic biomass for biofuels and bioproducts: an overview. Waste Biomass Valoriz 10:235–251
Sindhu R, Binod P, Pandey A (2016) Biological pretreatment of lignocellulosic biomass – an overview. Bioresour Technol 199:76–82
Sirohi S, Ummalyma SB, Sagar NA, Sharma P, Awasthi MK, Badgujar PC, Madhavan A, Rajasekharan R, Sindhu R, Sim SJ, Pandey A (2021) Strategies and advances in the pretreatment of microalgal biomass. J Biotechnol 341:63–75
Sulfahri SM, Husain DR, Langford A, Tassakka ACMAR (2020) Fungal pretreatment as a sustainable and low cost option for bioethanol production from marine algae. J Clean Prod 265:121763
Sun C, Xia A, Liao Q, Fu Q, Huang Y, Zhu X, Wei P, Lin R, Murphy JD (2018) Improving production of volatile fatty acids and hydrogen from microalgae and rice residue: effects of physicochemical characteristics and mix ratios. Appl Energy 230:1082–1092
Thomsen ST, Londoño JE, Ambye-Jensen M, Heiske S, Kádár Z, Meyer AS (2016) Combination of ensiling and fungal delignification as effective wheat straw pretreatment. Biotechnol Biofuels 9:16
Tsegaye B, Balomajumder C, Roy P (2019) Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect. Bull Natl Res Cent 43:51
Vidmar B, Logar RM, Panjičko M, Fanedl L (2017) Influence of thermal and bacterial pretreatment of microalgae on biogas production in mesophilic and thermophilic conditions. Acta Chim Slov 64:227–236
Wang M, Chen S, Zhou W, Yuan W, Wang D (2020) Algal cell lysis by bacteria: a review and comparison to conventional methods. Algal Res 46:101794
Wei S (2016) The application of biotechnology on the enhancing of biogas production from lignocellulosic waste. Appl Microbiol Biotechnol 100:9821–9836
Zabed HM, Akter S, Yun J, Zhang G, Awada FN, Qi X, Sahu JN (2019) Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production. Renew Sustain Energy Rev 105:105–128
Zabed HM, Akter S, Yun J, Zhang G, Zhang Y, Qi X (2020) Biogas from microalgae: technologies, challenges and opportunities. Renew Sustain Energy Rev 117:109503
Zhang Y, Kang X, Wang Z, Kong X, Li L, Sun Y, Zhu S, Feng S, Luo X, Lv P (2018) Enhancement of the energy yield from microalgae via enzymatic pretreatment and anaerobic co-digestion. Energy 164:400–407
Zhang Y, Li L, Kang X, Sun Y, Yuan Z, Xing T, Lin R (2019) Improving methane production from Pennisetum hybrid by monitoring plant height and ensiling pretreatment. Renew Energy 141:57–63
Zuorro A, Miglietta S, Familiari G, Lavecchia R (2016) Enhanced lipid recovery from Nannochloropsis microalgae by treatment with optimized cell wall degrading enzyme mixtures. Bioresour Technol 212:35–41
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Das, P., Baruah, J., Kalita, E. (2022). Recent Developments in the Enzymatic and Biocatalytic Pretreatment of Microalgae for Efficient Biofuel Production. In: Verma, P. (eds) Micro-algae: Next-generation Feedstock for Biorefineries. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-19-0793-7_8
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