Skip to main content

Recent Developments in the Enzymatic and Biocatalytic Pretreatment of Microalgae for Efficient Biofuel Production

  • Chapter
  • First Online:
Micro-algae: Next-generation Feedstock for Biorefineries

Part of the book series: Clean Energy Production Technologies ((CEPT))

  • 561 Accesses

Abstract

The rapid rise in CO2 emissions owing to fossil fuel combustion and increasing energy demands worldwide has fuelled the research for finding a viable substitute for fossil fuels. Microalgal biofuels, also known as 3G biofuels, hold the promise of being a sustainable yet economical substitute for conventional fossil fuels. The relatively untenable production of biofuels from food crops had its share of twofold complicit concerns regarding food security and economic viability. Microalgae on the other hand are compounded with benefits of CO2 sequestration and rapid biomass accumulation at low energy inputs, only to be restricted by the scale of biofuel recovery. Pretreatment strategies for microalgae have been the key driver for efficient microalgal biofuel production through mechanical, thermal, and chemical procedures. With the advent of pioneering approaches entailing enzymatic and microbial pretreatments alongside the conventional approaches, productivity has further increased. In this chapter, we summarize the recent developments in the enzymatic and biocatalytic pretreatment methods for improving the yield of valuable products from microalgae. The chapter also discusses the various factors affecting the pretreatment process and provides an understanding of the future perspectives for exploring newer prospects.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BP:

biofuel production

EP:

enzymatic pretreatment

BAP:

bacterial pretreatment

FP:

fungal pretreatment

SRF:

soft-rot fungi

WRF:

white-rot fungi

BRF:

brown-rot fungi

1G:

first generation

2G:

second generation

3G:

third generation

4G:

fourth generation

References

  • Adsul M, Sandhu SK, Singhania RR, Gupta R, Puri SK, Mathur A (2020) Designing a cellulolytic enzyme cocktail for the efficient and economical conversion of lignocellulosic biomass to biofuels. Enzyme Microb Technol 133:109442

    Article  CAS  PubMed  Google Scholar 

  • Agrawal K, Verma P (2020) Production optimization of yellow laccase from Stropharia sp. ITCC 8422 and enzyme-mediated depolymerization and hydrolysis of lignocellulosic biomass for biorefinery application. Biomass Conver Biorefinery 5:1–20

    Google Scholar 

  • Alavijeh RS, Karimi K, Wijffels RH, van den Berg C, Eppink M (2020) Combined bead milling and enzymatic hydrolysis for efficient fractionation of lipids, proteins and carbohydrates of Chlorella vulgaris microalgae. Bioresour Technol 309:123321

    Article  CAS  PubMed  Google Scholar 

  • Alexandropoulou M, Antonopoulou G, Fragkou E, Ntaikou I, Lyberatos G (2017) Fungal pretreatment of willow sawdust and its combination with alkaline treatment for enhancing biogas production. J Environ Manage 203:704–713

    Article  CAS  PubMed  Google Scholar 

  • Ali SS, Abomohra AE-F, Sun J (2017) Effective bio-pretreatment of sawdust waste with a novel microbial consortium for enhanced biomethanation. Bioresour Technol 238:425–432

    Article  CAS  PubMed  Google Scholar 

  • Ananthi V, Brindhadevi K, Pugazhendhi A, Arun A (2021) Impact of abiotic factors on biodiesel production by microalgae. Fuel 284:118962

    Article  CAS  Google Scholar 

  • Ananthi V, Pugazhendhi A, Arun A (2020) Efficacy of chemical factors on production and extraction of biodiesel by microalgae. Int J Energy Res 2020:1–14

    Google Scholar 

  • Anwar M, Lou S, Chen L, Li H, Hu Z (2019) Recent advancement and strategy on bio-hydrogen production from photosynthetic microalgae. Bioresour Technol 292:121972

    Article  CAS  PubMed  Google Scholar 

  • Aydin S, Yıldırım E, Ince O, Ince B (2017) Rumen anaerobic fungi create new opportunities for enhanced methane production from microalgae biomass. Algal Res 23:150–160

    Article  Google Scholar 

  • Bai M-D, Chen C-Y, Lu W-C, Wan H-P, Ho S-H, Chang J-S (2015) Enhancing the oil extraction efficiency of Chlorella vulgaris with cell-disruptive pretreatment using active extracellular substances from bacillus thuringiensis ITRI-G1. Biochem Eng J 101:185–190

    Article  CAS  Google Scholar 

  • Barati B, Zafar FF, Rupani PF, Wang S (2021) Bacterial pretreatment of microalgae and the potential of novel nature hydrolytic sources. Environ Technol Innov 21:101362

    Article  CAS  Google Scholar 

  • Barragán-Trinidad M, Carrillo-Reyes J, Buitrón G (2017) Hydrolysis of microalgal biomass using ruminal microorganisms as a pretreatment to increase methane recovery. Bioresour Technol 244(1):100–107

    Article  PubMed  CAS  Google Scholar 

  • Bhardwaj N, Agrawal K, Verma P (2020) Algal biofuels: an economic and effective alternative of fossil fuels. In: Srivastava N, Srivastava M, Mishra PK, Gupta VK (eds) Microbial strategies for techno-economic biofuel production. Springer, Singapore, pp 207–227

    Google Scholar 

  • Bhushan S, Rana MS, Bhandari M, Sharma AK, Simsek H, Prajapati SK (2021) Enzymatic pretreatment of algal biomass has different optimal conditions for biogas and bioethanol routes. Chemosphere 284:131264

    Article  CAS  PubMed  Google Scholar 

  • Çakmak EK, Ugurlu A (2020) Enhanced biogas production of red microalgae via enzymatic pretreatment and preliminary economic assessment. Algal Res 50:101979

    Article  Google Scholar 

  • Carmen M, Oana NN, Andreea-Daniela D, Eduard-Marius L (2019) Enzymatic pretreatment of algal biomass for enhanced conversion to biogas. J Eng Sci Innov 4(4):361–370

    Google Scholar 

  • Carrillo-Reyes J, Barragán-Trinidad M, Buitrón G (2016) Biological pretreatments of microalgal biomass for gaseous biofuel production and the potential use of rumen microorganisms: a review. Algal Res 18:341–351

    Article  Google Scholar 

  • Cavinato C, Ugurlu A, de Godos I, Kendir E, Gonzalez-Fernandez C (2017) Biogas production from microalgae. In: Gonzalez-Fernandez C, Muñoz R (eds) Microalgae-based biofuels and bioproducts from feedstock cultivation to end-products. Woodhead Publishing, New York, pp 155–182

    Chapter  Google Scholar 

  • Córdova O, Passos F, Chamy R (2019) Enzymatic pretreatment of microalgae: cell wall disruption, biomass solubilisation and methane yield increase. Appl Biochem Biotechnol 189:787–797

    Article  PubMed  CAS  Google Scholar 

  • Córdova O, Santis J, Ruiz-Fillipi G, Zuñiga ME, Fermoso FG, Chamy R (2018) Microalgae digestive pretreatment for increasing biogas production. Renew Sustain Energy Rev 82:2806–2813

    Article  CAS  Google Scholar 

  • Costa JAV, Freitas BCB, Moraes L, Zaparoli M, Morais MG (2020) Progress in the physicochemical treatment of microalgae biomass for value-added product recovery. Bioresour Technol 301:122727

    Article  CAS  PubMed  Google Scholar 

  • Dalena F, Senatore A, Basile M, Marino D, Basile A (2019) From sugars to ethanol-from agricultural wastes to algal sources. In: Basile A, Dalena F (eds) Second and third generation of feedstocks the evolution of biofuels. Elsevier, Amsterdam, pp 557–597

    Google Scholar 

  • Dar RA, Phutela UG (2020) Enzymatic and hydrothermal pretreatment of newly isolated Spirulina subsalsa BGLR6 biomass for enhanced biogas production. Waste Biomass Valoriz 11:3639–3651

    Article  CAS  Google Scholar 

  • Debnath C, Bandyopadhyay TK, Bhunia B, Mishra U, Narayanasamy S, Muthuraj M (2021) Microalgae: sustainable resource of carbohydrates in third-generation biofuel production. Renew Sustain Energy Rev 150:111464

    Article  CAS  Google Scholar 

  • Fang X, Li Q, Lin Y, Lin X, Dai Y, Guo Z, Pan D (2018) Screening of a microbial consortium for selective degradation of lignin from tree trimmings. Bioresour Technol 254:247–255

    Article  CAS  PubMed  Google Scholar 

  • Ferdeș M, Dincă MN, Moiceanu G, Zăbavă BS, Paraschiv G (2020) Microorganisms and enzymes used in the biological pretreatment of the substrate to enhance biogas production: a review. Sustainability 12(17):7205

    Article  CAS  Google Scholar 

  • Godbole V, Pal MK, Gautam P (2021) A critical perspective on the scope of interdisciplinary approaches used in fourth-generation biofuel production. Algal Res 58:102436

    Article  Google Scholar 

  • Goswami RK, Mehariya S, Verma P, Lavecchia R, Zuorro A (2020a) Microalgae-based biorefineries for sustainable resource recovery from wastewater. J Water Process Engin 40:101747

    Article  Google Scholar 

  • Goswami RK, Agrawal K, Mehariya S, Molino A, Musmarra D, Verma P (2020b) Microalgae-based biorefinery for utilization of carbon dioxide for production of valuable bioproducts. In: Kumar A, Sharma S (eds) Chemo-biological systems for CO2 utilization. CRC Press, New York, pp 203–228

    Chapter  Google Scholar 

  • He S, Fan X, Katukuri NR, Yuan X, Wang F, Guo R-B (2016) Enhanced methane production from microalgal biomass by anaerobic bio-pretreatment. Bioresour Technol 204:145–151

    Article  CAS  PubMed  Google Scholar 

  • He Y, Wu T, Wang X, Chen B, Chen F (2018) Cost-effective biodiesel production from wet microalgal biomass by a novel two-step enzymatic process. Bioresour Technol 268:583–591

    Article  CAS  PubMed  Google Scholar 

  • Hom-Diaz A, Passos F, Ferrer I, Vicent T, Blánquez P (2016) Enzymatic pretreatment of microalgae using fungal broth from Trametes versicolor and commercial laccase for improved biogas production. Algal Res 19:184–188

    Article  Google Scholar 

  • Hu J, Xue Y, Guo H, Gao M-t, Li J, Zhang S, Tsang YF (2017) Design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity. Bioresour Technol 227:247–255

    Article  CAS  PubMed  Google Scholar 

  • Kadir WNA, Lam MK, Uemura Y, Lim JW, Lee KT (2018) Harvesting and pretreatment of microalgae cultivated in wastewater for biodiesel production: a review. Energ Conver Manage 171:1416–1429

    Article  CAS  Google Scholar 

  • Kannah RY, Kavitha S, Karthikeyan OP, Rene ER, Kumar G, Banu JR (2021) A review on anaerobic digestion of energy and cost effective microalgae pretreatment for biogas production. Bioresour Technol 332:125055

    Article  CAS  Google Scholar 

  • Karim A, Islam MA, Khalid ZB, Faizal CKM, Khan MDMR, Yousuf A (2020) Microalgal cell disruption and lipid extraction techniques for potential biofuel production. In: Yousuf A (ed) Microalgae cultivation for biofuels production. Elsevier, Amsterdam, pp 129–147

    Chapter  Google Scholar 

  • Kavitha S, Kannah RY, Banu JR, Kaliappan S, Johnson M (2017) Biological disintegration of microalgae for biomethane recovery-prediction of biodegradability and computation of energy balance. Bioresour Technol 224(2):1367–1375

    Article  CAS  Google Scholar 

  • Kendir E, Ugurlu A (2018) A comprehensive review on pretreatment of microalgae for biogas production. Int J Energy Res 42:3711–3731

    Article  CAS  Google Scholar 

  • Lin C-Y, Lu C (2021) Development perspectives of promising lignocellulose feedstocks for production of advanced generation biofuels: a review. Renew Sustain Energy Rev 136:110445

    Article  CAS  Google Scholar 

  • Liu Y, Cruz-Morales P, Zargar A, Belcher MS, Pang B, Englund E, Dan Q, Yin K, Keasling JD (2021) Biofuels for a sustainable future. Cell 184:1636–1647

    Article  CAS  PubMed  Google Scholar 

  • Maffei G, Bracciale MP, Broggi A, Zuorro A, Santarelli ML, Lavecchia R (2018) Effect of an enzymatic treatment with cellulase and mannanase on the structural properties of Nannochloropsis microalgae. Bioresour Technol 249:592–598

    Article  CAS  PubMed  Google Scholar 

  • Magdalena JA, Ballesteros M, González-Fernandez C (2018) Efficient anaerobic digestion of microalgae biomass: proteins as a key macromolecule. Molecules 23:1098

    Article  PubMed Central  CAS  Google Scholar 

  • Maurya DP, Singla A, Negi S (2015) An overview of key pretreatment processes for biological conversion of lignocellulosic biomass to bioethanol. 3 Biotech 5:597–609

    Article  PubMed  PubMed Central  Google Scholar 

  • Meehnian H, Jana AK, Jana MM (2016) Effect of particle size, moisture content, and supplements on selective pretreatment of cotton stalks by Daedalea flavida and enzymatic saccharification. 3 Biotech 6:235

    Article  PubMed  PubMed Central  Google Scholar 

  • Mehariya S, Goswami RK, Verma P, Lavecchia R, Zuorro A (2021a) Integrated approach for wastewater treatment and biofuel production in microalgae biorefineries. Energies 14:2282

    Article  CAS  Google Scholar 

  • Mehariya S, Goswami RK, Karthikeysan OP, Verma P (2021b) Microalgae for high-value products: a way towards green nutraceutical and pharmaceutical compounds. Chemosphere 280:130553

    Article  CAS  PubMed  Google Scholar 

  • Moodley P (2021) 1- sustainable biofuels: opportunities and challenges. Appl Biotechnol Rev 2021:1–20

    Google Scholar 

  • Muñoz C, Hidalgo C, Zapata M, Jeison D, Riquelme C, Rivas M (2014) Use of cellulolytic marine bacteria for enzymatic pretreatment in microalgal biogas production. Appl Environ Microbiol 80(14):4199–4206

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Mustafa AM, Poulsen TG, Sheng K (2016) Fungal pretreatment of rice straw with Pleurotus ostreatus and Trichoderma reesei to enhance methane production under solid-state anaerobic digestion. Appl Energy 180:661–671

    Article  CAS  Google Scholar 

  • Mustafa AM, Poulsen TG, Xia Y, Sheng K (2017) Combinations of fungal and milling pretreatments for enhancing rice straw biogas production during solid-state anaerobic digestion. Bioresour Technol 224:174–182

    Article  CAS  PubMed  Google Scholar 

  • Nagappan S, Devendran S, Tsai P-C, Dahms H-U, Ponnusamy VK (2019) Potential of two-stage cultivation in microalgae biofuel production. Fuel 252:339–349

    Article  CAS  Google Scholar 

  • Nagarajan D, Changa J-S, Lee D-J (2020) Pretreatment of microalgal biomass for efficient biohydrogen production – recent insights and future perspectives. Bioresour Technol 2020:1–14

    Google Scholar 

  • Onumaegbua C, Mooneya J, Alaswadb A, Olabi AG (2018) Pretreatment methods for production of biofuel from microalgae biomass. Renew Sustain Energy Rev 93:16–26

    Article  CAS  Google Scholar 

  • Padmaperuma G, Kapoore RV, Gilmour DJ, Vaidyanathan S (2018) Microbial consortia: a critical look at microalgae co-cultures for enhanced biomanufacturing. Crit Rev Biotechnol 38:690–703

    Article  CAS  PubMed  Google Scholar 

  • Passos F, Hom-Diaz A, Blanquez P, Vicent T, Ferrer I (2016) Improving biogas production from microalgae by enzymatic pretreatment. Bioresour Technol 199:347–351

    Article  CAS  PubMed  Google Scholar 

  • Paul V, Rai S, Tripathi AD, Rai DC, Agarwal A (2021) Impact of fermentation types on enzymes used for biofuels production. In: Srivastava N, Srivastava M, Mishra PK, Gupta VK (eds) Bioprocessing for biofuel production strategies to improve process parameters. Springer, Singapore, pp 1–27

    Google Scholar 

  • Raven S, Ekka SB, Chattree SE, Sadanand SS, Rina L, Tiwari A (2021) Microbial technology for biofuel production. In: Srivastava N, Srivastava M (eds) Bioenergy research: evaluating strategies for commercialization and sustainability. Wiley, New York, pp 19–45

    Chapter  Google Scholar 

  • Rodriguez C, Alaswad A, Benyounis KY, Olabi AG (2017) Pretreatment techniques used in biogas production from grass. Renew Sustain Energy Rev 68:1193–1204

    Article  CAS  Google Scholar 

  • Rouches E, Herpoël-Gimbert I, Steyer JP, Carrere H (2016) Improvement of anaerobic degradation by white-rot fungi pretreatment of lignocellulosic biomass: a review. Renew Sustain Energy Rev 59:179–198

    Article  CAS  Google Scholar 

  • Sadvakasova AK, Kossalbayev BD, Zayadan BK, Kirbayeva DK, Alwasel S, Allakhverdiev SI (2021) Potential of cyanobacteria in the conversion of wastewater to biofuels. World J Microbiol Biotechnol 37:140

    Article  CAS  PubMed  Google Scholar 

  • Saha BC, Kennedy GJ, Qureshi N, Cotta MA (2017) Biological pretreatment of corn Stover with Phlebia brevispora NRRL-13108 for enhanced enzymatic hydrolysis and efficient ethanol production. Biotechnol Prog 33:365–374

    Article  CAS  PubMed  Google Scholar 

  • Sangwijit K, Jitonnom J, Pitakrattananukool S, Yu LD, Anuntalabhochai S (2016) Low-energy plasma immersion ion implantation modification of bacteria to enhance hydrolysis of biomass materials. Surf Coat Technol 306:336–340

    Article  CAS  Google Scholar 

  • Sankaran R, Cruz RAP, Pakalapati H, Show PL, Ling TC, Chen WH, Tao Y (2020) Recent advances in the pretreatment of microalgal and lignocellulosic biomass: a comprehensive review. Bioresour Technol 298:122476

    Article  CAS  PubMed  Google Scholar 

  • Sharma HK, Xu C, Qin W (2019) Biological pretreatment of lignocellulosic biomass for biofuels and bioproducts: an overview. Waste Biomass Valoriz 10:235–251

    Article  CAS  Google Scholar 

  • Sindhu R, Binod P, Pandey A (2016) Biological pretreatment of lignocellulosic biomass – an overview. Bioresour Technol 199:76–82

    Article  CAS  PubMed  Google Scholar 

  • Sirohi S, Ummalyma SB, Sagar NA, Sharma P, Awasthi MK, Badgujar PC, Madhavan A, Rajasekharan R, Sindhu R, Sim SJ, Pandey A (2021) Strategies and advances in the pretreatment of microalgal biomass. J Biotechnol 341:63–75

    Article  CAS  PubMed  Google Scholar 

  • Sulfahri SM, Husain DR, Langford A, Tassakka ACMAR (2020) Fungal pretreatment as a sustainable and low cost option for bioethanol production from marine algae. J Clean Prod 265:121763

    Article  CAS  Google Scholar 

  • Sun C, Xia A, Liao Q, Fu Q, Huang Y, Zhu X, Wei P, Lin R, Murphy JD (2018) Improving production of volatile fatty acids and hydrogen from microalgae and rice residue: effects of physicochemical characteristics and mix ratios. Appl Energy 230:1082–1092

    Article  CAS  Google Scholar 

  • Thomsen ST, Londoño JE, Ambye-Jensen M, Heiske S, Kádár Z, Meyer AS (2016) Combination of ensiling and fungal delignification as effective wheat straw pretreatment. Biotechnol Biofuels 9:16

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Tsegaye B, Balomajumder C, Roy P (2019) Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect. Bull Natl Res Cent 43:51

    Article  Google Scholar 

  • Vidmar B, Logar RM, Panjičko M, Fanedl L (2017) Influence of thermal and bacterial pretreatment of microalgae on biogas production in mesophilic and thermophilic conditions. Acta Chim Slov 64:227–236

    Article  CAS  PubMed  Google Scholar 

  • Wang M, Chen S, Zhou W, Yuan W, Wang D (2020) Algal cell lysis by bacteria: a review and comparison to conventional methods. Algal Res 46:101794

    Article  Google Scholar 

  • Wei S (2016) The application of biotechnology on the enhancing of biogas production from lignocellulosic waste. Appl Microbiol Biotechnol 100:9821–9836

    Article  CAS  PubMed  Google Scholar 

  • Zabed HM, Akter S, Yun J, Zhang G, Awada FN, Qi X, Sahu JN (2019) Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production. Renew Sustain Energy Rev 105:105–128

    Article  CAS  Google Scholar 

  • Zabed HM, Akter S, Yun J, Zhang G, Zhang Y, Qi X (2020) Biogas from microalgae: technologies, challenges and opportunities. Renew Sustain Energy Rev 117:109503

    Article  CAS  Google Scholar 

  • Zhang Y, Kang X, Wang Z, Kong X, Li L, Sun Y, Zhu S, Feng S, Luo X, Lv P (2018) Enhancement of the energy yield from microalgae via enzymatic pretreatment and anaerobic co-digestion. Energy 164:400–407

    Article  CAS  Google Scholar 

  • Zhang Y, Li L, Kang X, Sun Y, Yuan Z, Xing T, Lin R (2019) Improving methane production from Pennisetum hybrid by monitoring plant height and ensiling pretreatment. Renew Energy 141:57–63

    Article  CAS  Google Scholar 

  • Zuorro A, Miglietta S, Familiari G, Lavecchia R (2016) Enhanced lipid recovery from Nannochloropsis microalgae by treatment with optimized cell wall degrading enzyme mixtures. Bioresour Technol 212:35–41

    Article  CAS  PubMed  Google Scholar 

Download references

Competing Interests

All the authors declare that they have no competing interests.

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Das, P., Baruah, J., Kalita, E. (2022). Recent Developments in the Enzymatic and Biocatalytic Pretreatment of Microalgae for Efficient Biofuel Production. In: Verma, P. (eds) Micro-algae: Next-generation Feedstock for Biorefineries. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-19-0793-7_8

Download citation

Publish with us

Policies and ethics