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Effects of Halophilic Bacteria on Biochemical Characteristics of Rice Cultivars Under Salinity Stress Conditions

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Saline Soil-based Agriculture by Halotolerant Microorganisms

Abstract

Soil salinization is a serious ecological problem affecting cereal crops including rice. Different bacterial strains have been identified and used as salt remediator in salt-affected soil, while there is confined use of halophilic bacteria. Present study was aimed to find the growth stimulatory effects of two salt-resistant Bacillus strains (NCCP-71 and NCCP-77) on biochemical changes in rice cultivars (NIAB-IR-9 and KSK-282) under control (0 mM) and different salt stress treatments (50, 100, and 150 Mm NaCl). High concentration of salinity increased the Na+ ion content and lowered the K+ and Ca+ ion contents in rice. The rice seedlings inoculated with Bacillus strains showed a decrease in Na+ content followed by an increase in K+ ion content. Calcium ions were increased by NCCP-71 in both varieties; however NCCP-77 inoculation decreased calcium ion content in KSK-282 and in NIAB-IR-9 at 50 mM NaCl. Salinity gradually lowered the contents of photosynthetic pigments; however inoculation with NCCP-71 and NCCP-77 caused a prominent increase in photosynthetic content (Chl a, Chl b, and carotenoids) under salt stress. On the other side, significant reduction in protein and nitrogen occurred with increasing salt stress. Inoculation with bacterial strains NCCP-71 enhanced protein and nitrogen content in both varieties, while inoculation with NCCP-77 enhanced nitrogen and protein content at 50 mM NaCl concentration. Both halophilic bacteria had a great impact on the biochemical characteristics of inoculated rice cultivar by modifying different biochemical processes under saline conditions.

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Authors and Affiliations

  1. Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

    Mehvish Riaz Khattak, Sami Ullah Jan, Ijaz Malook, Sehrish Riaz Khattak, Nazneen Akhtar, Sehresh Khan & Muhammad Jamil

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  1. Mehvish Riaz Khattak
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  2. Sami Ullah Jan
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  3. Ijaz Malook
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  4. Sehrish Riaz Khattak
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  5. Nazneen Akhtar
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  6. Sehresh Khan
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  7. Muhammad Jamil
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Correspondence to Muhammad Jamil .

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Editors and Affiliations

  1. Department of Life Sciences, Central University of Jharkhand, Ranchi, Jharkhand, India

    Manoj Kumar

  2. Department of Soil Science, University of Tehran, Tehran, Iran

    Hassan Etesami

  3. Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand, India

    Vivek Kumar

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Khattak, M.R. et al. (2019). Effects of Halophilic Bacteria on Biochemical Characteristics of Rice Cultivars Under Salinity Stress Conditions. In: Kumar, M., Etesami, H., Kumar, V. (eds) Saline Soil-based Agriculture by Halotolerant Microorganisms. Springer, Singapore. https://doi.org/10.1007/978-981-13-8335-9_7

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