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Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions

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Abstract

Ethylene synthesis is accelerated in response to various environmental stresses like salinity. Ten rhizobacterial strains isolated from wheat rhizosphere taken from different salt affected areas were screened for growth promotion of wheat under axenic conditions at 1, 5, 10 and 15 dS m−1. Three strains, i.e., Pseudomonas putida (N21), Pseudomonas aeruginosa (N39) and Serratia proteamaculans (M35) showing promising performance under axenic conditions were selected for a pot trial at 1.63 (original), 5, 10 and 15 dS m−1. Results showed that inoculation was effective even in the presence of higher salinity levels. P. putida was the most efficient strain compared to the other strains and significantly increased the plant height, root length, grain yield, 100-grain weight and straw yield up to 52, 60, 76, 19 and 67%, respectively, over uninoculated control at 15 dS m−1. Similarly, chlorophyll content and K+/Na+ of leaves also increased by P. putida over control. It is highly likely that under salinity stress, 1-aminocyclopropane-1-carboxylic acid-deaminase activity of these microbial strains might have caused reduction in the synthesis of stress (salt)-induced inhibitory levels of ethylene. The results suggested that these strains could be employed for salinity tolerance in wheat; however, P. putida may have better prospects in stress alleviation/reduction.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

CFU:

Colony-forming unit

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Acknowledgments

The work presented in this manuscript was financially supported by Higher Education Commission (HEC), Islamabad, Pakistan.

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Authors and Affiliations

  1. Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, 38040, Pakistan

    Zahir Ahmad Zahir, Usman Ghani, Muhammad Naveed, Sajid Mahmood Nadeem & Hafiz Naeem Asghar

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  1. Zahir Ahmad Zahir
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  2. Usman Ghani
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  3. Muhammad Naveed
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  4. Sajid Mahmood Nadeem
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  5. Hafiz Naeem Asghar
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Correspondence to Zahir Ahmad Zahir.

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Communicated by Jorge Membrillo-Hernández.

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Zahir, Z.A., Ghani, U., Naveed, M. et al. Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions. Arch Microbiol 191, 415–424 (2009). https://doi.org/10.1007/s00203-009-0466-y

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