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High Concentration Vinegar Production by Acetic Acid Bacteria Using Edible Alcohol as Feedstock

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Advances in Applied Biotechnology (ICAB 2016)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 444))

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Abstract

The high concentration vinegar plays an important role in condiment industry and daily life. Its demand significantly increase. In this work, an optimized process for high concentration vinegar production by AAB.WUST-01 using edible alcohol as feedstock was developed. The influences of carbon source, nitrogen source and ethanol concentrations on the biomass growth were investigated in starting-up stage in vinegar fermentation. The optimized medium composition was obtained. The optimum value for glucose, yeast extract and ethanol were about 5.1 g/L, yeast extract 26.2 g/L, and ethanol 10 mL/L respectively. In such a condition, the biomass is increased 4.34 fold from 0.780 to 4.16 g/L. Also, we discussed the influences of ethanol concentrations to the vinegar acidity in the acid producing stage. The result showed that ethanol in the fermentation would be 50 mL/L, the corresponding total acid could reach 45.71 g/L, and the yield reached up to 88.76%.

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References

  1. Berraud C (2000) Production of highly concentrated vinegar in fed-batch culture. Biotechnol Lett 22:451–454

    Article  CAS  Google Scholar 

  2. Dorini FA, Cecconello MS, Dorini LB (2016) On the logistic equation subject to uncertainties in the environmental carrying capacity and initial population density. Commun Nonlinear Sci Numer Simulat 33:160–173

    Article  Google Scholar 

  3. Janja T, Francios B (2015) Updates on quick identification of acetic acid bacteria with a focus on the 16S–23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry. Int J Food Microbiol 196:137–144

    Article  Google Scholar 

  4. Lee CY, Nakano A, Naofumi S (2003) Effects of substrate feed rates on heterologous protein expression by Pichia pastoris in DO-stat fed-batch fermentation. Enzyme Microb Technol 33:358–365

    Article  CAS  Google Scholar 

  5. Liu JZ, Weng LP, Zhang QL et al (2003) A mathematical model for gluconic acid fermentation by Aspergillus niger. Biochem Eng J 14:137–141

    Article  CAS  Google Scholar 

  6. Mamlouk D, Gullo M (2013) Acetic acid bacteria: physiology and carbon sources oxidation. Indian J Microbiol 53(4):377–384

    Article  CAS  Google Scholar 

  7. Mas A, Torija M, Troncoso A et al (2014) Acetic acid bacteria and the production and quality of wine vinegar. Sci World J 2:1–6

    Article  Google Scholar 

  8. Mitsukura K, Uno T, Yoshida T (2007) Microbial asymmetric oxidation of 2-butyl-1, 3-propanediol. Appl Microbiol Biotechnol 76(1):61–65

    Article  CAS  Google Scholar 

  9. Matsushita K, Kobayashi Y, Mizuguchi M et al (2008) A tightly bound quinone functions in the ubiquinone reaction sites of quinoprotein alcohol dehydrogenase of an acetic acid bacterium Gluconobacter suboxydans. Biosci Biotechnol Biochem 72(10):2723–2731

    Article  CAS  Google Scholar 

  10. Natsaran S, Kazunobu M, Osao A et al (2015) Acetic acid bacteria: A group of bacteria with versatile biotechnological applications. Biotechnol Adv 33:1260–1271

    Article  Google Scholar 

  11. Qi Z, Yang H, Yu X (2013) A protocol for optimization vinegar fermentation according to the ratio of oxygen consumption versus acid yield. J Food Eng 116:304–309

    Article  CAS  Google Scholar 

  12. Sofia L, Efimia H, Maria P et al (2015) Beyond traditional balsamic vinegar: compositional and sensorial characteristics of industrial balsamic vinegars and regulatory requirements. J Food Compos Anal 43:175–184

    Article  Google Scholar 

  13. Torija M, Mateo E, Vegas C (2009) Effect of wood type and thickness on acetification kinetics in traditional vinegar production. Int J Wine Res 1(1):155–160

    CAS  Google Scholar 

  14. Toshiharu Y (2010) Alcohol dehydrogenase of acetic acid bacteria: structure, mode of action, and applications in biotechnology. Appl Microbiol Biotechnol 86:1257–1265

    Article  Google Scholar 

  15. Yang HL, Qi ZL, Xia XL et al (2011) An optimum medium designed and verified for alcohol vinegar fermentation. Afr J Biotechnol 10(42):8421–8427

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The present work was financed by the National Natural Science Foundation of China (Grant no. 21376184), the Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry), Foundation from Educational Commission of Hubei Province of China (Grant no. D20121108) and the Innovative Team of Bioaugmentation and Advanced Treatment on Metallurgical Industry Wastewater.

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Correspondence to Zhong-Hua Yang .

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Yin, XY., Zhai, R., Zhong, WK., Huo, J., Yang, ZH. (2018). High Concentration Vinegar Production by Acetic Acid Bacteria Using Edible Alcohol as Feedstock. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_46

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