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3D Bioprinting for Tissue Engineering

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Clinical Regenerative Medicine in Urology

Abstract

Three-dimensional (3D) printing, also referred to as additive manufacturing (AM), is being used widely in diverse areas, including manufacturing, food, art, and architecture. Recently, regenerative medicine has adopted the technology to produce computer-designed cellular structures with cells, biomaterials, and signals for artificial tissue/organ regeneration. In this context, it is referred to as 3D bioprinting. To date, promising results have been reported, with the successful regeneration of artificial vessels, bones, and ear tissues. Moreover, bioprinting technology is a useful tool in biomedical applications, such as bio-chips, biosensors, and drug delivery systems. This chapter includes two main sections. The first briefly describes three bioprinting techniques: jetting-, extrusion-, and laser-based bioprinting. The techniques are compared in terms of working principles, components, advantages, disadvantages, and applicable bio-inks. The second section describes bioprinting’s application to tissue and biomedical engineering.

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Abbreviations

BMSC:

Bone marrow stem cells

CTZ:

Cetirizine HCl

DAT:

Decellularized adipose tissue

DPD:

Diphenylhydramine HCl

ECM:

Endothelial cell medium

FM:

Fibroblast medium

GelMA:

Gelatin methacrylate

GS:

Gentamicin sulfate

HA:

Hyaluronic acid

hASCs:

Human adipose tissue-derived mesenchymal stem cells

hCMPCs:

Human cardiac-derived cardiomyocyte progenitor cells

hESCs:

Human embryonic stem cells

HFF-1:

Neonatal human foreskin fibroblasts

hiPSCs:

Human induced pluripotent stem cells

HUVECs:

Human umbilical vein endothelial cells

HUVSMC:

Human umbilical vein smooth muscle cells

IBU:

Ibuprofen

ihMSCs:

Immortalized human mesenchymal stem cells

MSCs:

Mesenchymal stromal cells

MTX:

Methotrexate

MWNTs:

Multi walled carbon nanotubes

n-HA:

nano-Hydroxyapatite

NHDFs:

Normal human dermal fibroblasts

NHLFs:

Normal human lung fibroblasts

PCL:

Polycaprolactone

PDLLA:

Poly(d,l-lactic acid or d,l-lactide)

PDMS:

Polydimethylsiloxane

PLGA:

Poly(lactic-co-glycolic acid)

PLLA:

Poly-l-lactide

PU:

Polyurethane

PVA:

Polyvinyl alcohol

SCs:

Schwann cells

SF-PGA:

Silk fibroin-poly glutamic acid

SF-PLL:

Silk fibroin-poly-l-lysine

SMCM:

Smooth muscle cell medium

SMCs:

Smooth muscle cells

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Authors and Affiliations

  1. School of Life Science, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Ulju-gun, 44919, Ulsan, South Korea

    Sujin Noh, Noehyun Myung, Myeongji Park, Seulgi Kim & Hyun-Wook Kang

  2. Department of Mechanical Engineering, Dong-Eui University, 176 Eomgwangro, Busanjin-gu, 47340, Busan, South Korea

    Sung-Uk Zhang

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  1. Sujin Noh
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Correspondence to Hyun-Wook Kang .

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  1. Department of Urology, Kyungpook National University Hospital, Daegu, Korea (Republic of)

    Bup Wan Kim

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Noh, S., Myung, N., Park, M., Kim, S., Zhang, SU., Kang, HW. (2018). 3D Bioprinting for Tissue Engineering. In: Kim, B. (eds) Clinical Regenerative Medicine in Urology. Springer, Singapore. https://doi.org/10.1007/978-981-10-2723-9_5

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