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Development of 3D PPF/DEF scaffolds using micro-stereolithography and surface modification

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Abstract

Poly(propylene fumarate) (PPF) is an ultraviolet-curable and biodegradable polymer with potential applications for bone regeneration. In this study, we designed and fabricated three-dimensional (3D) porous scaffolds based on a PPF polymer network using micro-stereolithography (MSTL). The 3D scaffold was well fabricated with a highly interconnected porous structure and porosity of 65%. These results provide a new scaffold fabrication method for tissue engineering. Surface modification is a commonly used and effective method for improving the surface characteristics of biomaterials without altering their bulk properties that avoids the expense and long time associated with the development of new biomaterials. Therefore, we examined surface modification of 3D scaffolds by applying accelerated biomimetic apatite and arginine-glycine-aspartic acid (RGD) peptide coating to promote cell behavior. The apatite coating uniformly covered the scaffold surface after immersion for 24 h in 5-fold simulated body fluid (5SBF) and then the RGD peptide was applied. Finally, the coated 3D scaffolds were seeded with MC3T3-E1 pre-osteoblasts and their biologic properties were evaluated using an MTS assay and histologic staining. We found that 3D PPF/diethyl fumarate (DEF) scaffolds fabricated with MSTL and biomimetic apatite coating can be potentially used in bone tissue engineering.

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Acknowledgments

This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the National R&D Project (M10646020003–06N4602–00310), the National Research Laboratory Program (R0A–2005–000–10042–0(2007)), and National R&D Project for Nano Science and Technology (M10203000020–07M0300–02010) funded by the Ministry of Science and Technology.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Hanoi University of Technology, 1A-Dai Co Viet Street, Hai Ba Trung District, Hanoi City, Vietnam

    Phung Xuan Lan

  2. Department of Mechanical Engineering, POSTECH, Pohang, Gyeongbuk, 790-784, Republic of Korea

    Jin Woo Lee, Young-Joon Seol & Dong-Woo Cho

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  1. Phung Xuan Lan
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  2. Jin Woo Lee
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  3. Young-Joon Seol
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  4. Dong-Woo Cho
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Correspondence to Dong-Woo Cho.

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Lan, P.X., Lee, J.W., Seol, YJ. et al. Development of 3D PPF/DEF scaffolds using micro-stereolithography and surface modification. J Mater Sci: Mater Med 20, 271–279 (2009). https://doi.org/10.1007/s10856-008-3567-2

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  • DOI: https://doi.org/10.1007/s10856-008-3567-2

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