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Plant Regeneration and Genetic Transformation in Jatropha

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Biotechnology of Neglected and Underutilized Crops

Abstract

Jatropha curcas, a non-edible oil bearing species with multiple uses, and considerable economic potential is emerging as a potential biofuel plant. The limited knowledge of this species, low and inconsistent yields, the narrow genetic variability, and vulnerability to insects and diseases are major constraints in successful cultivation of Jatropha as a biofuel crop. Hence, genetic improvement of Jatropha is essential by conventional and modern biotechnological tools to use as a viable alternative source of bio-diesel. Realising its potential as a bio-energy crop, in vitro regeneration methods have been established to meet the demand of large scale supply of superior clones, and also as a prelude for genetic improvement of the species through transgenic approaches. In this chapter, an overview of in vitro tissue culture and genetic transformation of Jatropha is discussed.

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Abbreviations

NAA:

α-napthaleneacetic acid

BA:

Benzyl adenine

MS:

Murashige and Skoog

Kn:

6-furfurylaminopurine

IBA:

Indole-3-butyric acid

TDZ:

Thidiazuron

AFLP:

Amplified fragment length polymorphism

ISSRs:

Inter simple sequence repeats

MSAP:

Methylation sensitive amplified polymorphism

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Authors and Affiliations

  1. Directorate of Oilseeds Research, Rajendranagar, Hyderabad, Andhra Pradesh, 500 030, India

    M. Sujatha

  2. Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamilnadu, 641 046, India

    S. Nithianantham

  3. Plant Stress Genomics Research Centre, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia

    M. P. Reddy

Authors
  1. M. Sujatha
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  2. S. Nithianantham
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  3. M. P. Reddy
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Corresponding author

Correspondence to M. Sujatha .

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Editors and Affiliations

  1. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. Dept. Agricultural & Food Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    S. Dutta Gupta

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Sujatha, M., Nithianantham, S., Reddy, M.P. (2013). Plant Regeneration and Genetic Transformation in Jatropha . In: Jain, S., Dutta Gupta, S. (eds) Biotechnology of Neglected and Underutilized Crops. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5500-0_13

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