Overview
- Presents state of the art information on NMR spectroscopy, and its place in the broader field of biophysics
- No other monograph presents such a wide range of topics, including NMR spectroscopy, protein folding, X-ray crystallography, spectroscopy and applications
Part of the book series: NATO Science for Peace and Security Series B: Physics and Biophysics (NAPSB)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11–13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16–18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.
Similar content being viewed by others
Keywords
Table of contents (8 papers)
-
Front Matter
-
Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: Biophysics and the Challenges of Emerging Threats
Editors: Joseph D. Puglisi
Series Title: NATO Science for Peace and Security Series B: Physics and Biophysics
DOI: https://doi.org/10.1007/978-90-481-2368-1
Publisher: Springer Dordrecht
eBook Packages: Physics and Astronomy, Physics and Astronomy (R0)
Copyright Information: Springer Science+Business Media B.V. 2009
Hardcover ISBN: 978-90-481-2366-7Published: 05 June 2009
Softcover ISBN: 978-90-481-2367-4Published: 05 June 2009
eBook ISBN: 978-90-481-2368-1Published: 25 May 2009
Series ISSN: 1874-6500
Series E-ISSN: 1874-6535
Edition Number: 1
Number of Pages: VII, 179
Topics: Life Sciences, general, Biological and Medical Physics, Biophysics, Biotechnology, Solid State Physics, Spectroscopy and Microscopy, Theoretical and Computational Chemistry