Abstract
In this chapter, we present dynamic structures of peripheral nerve fibers under various stretching conditions and distribution of soluble serum proteins with “in vivo cryotechnique” (IVCT). The structures of IVCT-prepared fibers under the stretched condition showed a beaded appearance. By immunostaining for a membrane skeletal protein 4.1G, Schmidt-Lanterman incisures (SLIs) were clearly identified, and the heights of their circular truncated cones were increased at narrow sites of the fibers under the stretched condition. Albumin was immunolocalized in blood vessels and the endoneurium including near the node of Ranvier. Thus, IVCT revealed structures and accurate immunolocalization of serum albumin in the sciatic nerve fibers, reflecting living states.
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Kamijo, A., Saitoh, Y., Ohno, N., Ohno, S., Terada, N. (2016). Application of “In Vivo Cryotechnique” to Immunohistochemical Study of Mouse Sciatic Nerves Under Various Stretching Conditions. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_37
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DOI: https://doi.org/10.1007/978-4-431-55723-4_37
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-55722-7
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