In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

  • Shinichi Ohno
  • Nobuhiko Ohno
  • Nobuo Terada

Table of contents

  1. Front Matter
    Pages i-xi
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Shinichi Ohno
      Pages 3-5
    3. Shinichi Ohno
      Pages 11-14
    4. Shinichi Ohno
      Pages 15-16
  3. Application of ‘IVCT’ to Various Mouse Organs

    1. Front Matter
      Pages 17-17
    2. Nobuhiko Ohno, Nobuo Terada, Shinichi Ohno
      Pages 19-23
    3. Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 25-28
    4. Satoshi Shimo, Sei Saitoh, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 33-38
    5. Jiaorong Chen, Nobuo Terada, Yurika Saitoh, Zheng Huang, Nobuhiko Ohno, Shinichi Ohno
      Pages 39-44
    6. Yuqin Bai, Bao Wu, Nobuo Terada, Nobuhiko Ohno, Yurika Saitoh, Shinichi Ohno
      Pages 45-49
    7. Sei Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 51-56
    8. Liye Shi, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 67-76
    9. Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 77-81
    10. Shinichi Ohno, Nobuo Terada, Yasuhisa Fujii
      Pages 83-86
    11. Yuqin Bai, Bao Wu, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Sei Saitoh et al.
      Pages 87-92

About this book

Introduction

This book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.

Keywords

Cryostat section Immunocytochemistry Live imaging Living Morphology Microscope Analysis

Editors and affiliations

  • Shinichi Ohno
    • 1
  • Nobuhiko Ohno
    • 2
  • Nobuo Terada
    • 3
  1. 1.Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and EngineeringUniversity of YamanashiYamanashiJapan
  2. 2.Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and EngineeringUniversity of YamanashiYamanashiJapan
  3. 3.Division of Health ScienceShinshu University Graduate School of MedicineNaganoJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-4-431-55723-4
  • Copyright Information Springer Japan 2016
  • Publisher Name Springer, Tokyo
  • eBook Packages Medicine
  • Print ISBN 978-4-431-55722-7
  • Online ISBN 978-4-431-55723-4
  • About this book