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Class A GPCR: Serotonin Receptors

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G-Protein-Coupled Receptor Dimers

Part of the book series: The Receptors ((REC,volume 33))

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Abstract

Hundreds of studies have reported that G protein-coupled receptors self-associate to form dimers or oligomers. Yet, this topic remains controversial. This chapter reviews the current literature related to the structure and function of 5-HT receptor dimers/oligomers in recombinant cells and in native tissues. Studies designed to examine the functional significance of 5-HT receptor dimer/oligomer formation are evaluated and discussed. Emphasis is placed on the methods employed, the dimer interface, oligomer size, mechanism of G protein activation, and analysis of bivalent ligands as potential therapeutics.

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Abbreviations

5-HT:

serotonin

AA:

arachidonic acid

BiFC:

bimolecular fluorescence complementation

BRET:

bioluminescence resonance energy transfer

Co-IP:

co-immunoprecipiation

FCS:

fluorescence correlation spectroscopy

FISH:

fluorescent in situ hybridization

FRET:

fluorescence resonance energytransfer

GPCR:

G protein-coupled receptor

IP:

inositol phosphate

PLA:

proximity ligation assay

SpIDA:

spatial intensity distribution ananlysis

TCSPC:

time correlated single photon counting

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  1. Department of Neuroscience and Experimental Therapeutics MC-136, Albany Medical College, 47 New Scotland Avenue, Albany, NY, 12208, USA

    Ellinor Grinde M.S. & Katharine Herrick-Davis Ph.D.

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  2. Katharine Herrick-Davis Ph.D.
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  1. Department of Neuroscience and Experimental Therapeutics, Albany Medical College, Albany, New York, USA

    Katharine Herrick-Davis

  2. Centre for Translational Pharmacology, Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Graeme Milligan

  3. Department of Physiology and Biochemistry, University of Malta, Msida, Malta

    Giuseppe Di Giovanni

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Grinde, E., Herrick-Davis, K. (2017). Class A GPCR: Serotonin Receptors. In: Herrick-Davis, K., Milligan, G., Di Giovanni, G. (eds) G-Protein-Coupled Receptor Dimers. The Receptors, vol 33. Humana Press, Cham. https://doi.org/10.1007/978-3-319-60174-8_6

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