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Detection and Diagnosis of PVY

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Potato virus Y: biodiversity, pathogenicity, epidemiology and management

Abstract

The worldwide prevalence of Potato virus Y (PVY) poses a continuous challenge to efficient potato production. The accurate diagnosis of viruses such as PVY is inherently challenging due to the broad biological and genetic diversity of PVY strains that elicit a range of symptoms and diseases in various potato cultivars and related solanaceous species. A wide range of techniques have been developed over a period of 50 years for the detection of PVY. Serological methods such as ELISA, using polyclonal and especially monoclonal antibodies, have been widely used by most diagnostic laboratories, due to their cost effectiveness and capacity to implement for a large number of samples. Over the last decade, PCR-based assays have been routinely used in diagnostic laboratories because of their sensitivity, specificity and their capacity to be automated for high-throughput testing. The objective of this chapter is to provide a brief historical overview of the main diagnostic methods used to detect and identify PVY and to highlight those that are suitable for either research or diagnostic purposes.

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Authors and Affiliations

  1. FN3PT/RD3PT, 43-45 rue de Naples, F-75008, Paris, France

    Laurent Glais

  2. IGEPP, Agrocampus Ouest, INRA, Université de Rennes 1, F-35650, Le Rheu, France

    Laurent Glais

  3. Department of Plant Soil and Entomological Sciences, University of Idaho, 875 Perimeter Drive MS 2339, Moscow, ID, 83844-2339, USA

    Mohamad Chikh Ali & Alexander V. Karasev

  4. Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia

    Denis Kutnjak

  5. Science and Advice for Scottish Agriculture (SASA), 1 Roddinglaw Road, Edinburgh, EH12 9FJ, UK

    Christophe Lacomme

Authors
  1. Laurent Glais
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  2. Mohamad Chikh Ali
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  3. Alexander V. Karasev
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  4. Denis Kutnjak
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  5. Christophe Lacomme
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Corresponding author

Correspondence to Laurent Glais .

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Editors and Affiliations

  1. Virology & Zoology, Science and Advice for Scottish Agriculture, Edinburgh, United Kingdom

    Christophe Lacomme

  2. IGEPP, Agrocampus Ouest, INRA, Université de Rennes 1, Le Rheu, France

    Laurent Glais

  3. Department of Biochemistry, Stellenbosch University, Matieland, South Africa

    Dirk U. Bellstedt

  4. Field crops, Agroscope Field crops, Nyon 1, Switzerland

    Brice Dupuis

  5. Plant, Soil and Entomological Sciences, University of Idaho, Moscow, Idaho, USA

    Alexander V. Karasev

  6. UMR-BGPI TA A-54/K, CIRAD, Montpellier Cedex 5, France

    Emmanuel Jacquot

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Glais, L., Chikh Ali, M., Karasev, A.V., Kutnjak, D., Lacomme, C. (2017). Detection and Diagnosis of PVY. In: Lacomme, C., Glais, L., Bellstedt, D., Dupuis, B., Karasev, A., Jacquot, E. (eds) Potato virus Y: biodiversity, pathogenicity, epidemiology and management. Springer, Cham. https://doi.org/10.1007/978-3-319-58860-5_5

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