Differentiation of Stem Cells into Hepatocyte Lineage: In Vitro Cell Culture, In Vivo Transplantation in Animal Models

Alomari, Munther

doi:10.1007/978-3-030-78101-9_6

Munther Alomari³

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL,volume 69))

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Abstract

Liver failure is one of the life-threatening illnesses, and it accounts for 3.5% of all deaths worldwide. Liver transplantation is the solution, but due to the long waiting list for an available donor, patients die before they get the chance to get a healthy liver. Shortage supply of donor organs, lifelong need for immunosuppression, and the adult hepatocytes culturing difficulty are the serious limitations for liver transplantation. Therefore, hepatocyte transplantation of stem cells with tissue engineering is the alternative therapeutic approach to liver transplantation. Differentiation of stem cells from many origins into hepatocytes has been reported for therapeutic and research purposes. This book chapter summarizes the differentiation process into hepatocytes from different stem cell types, including mesenchymal, pluripotent, hematopoietic, and umbilical cord stem cells. In addition, it discusses the use of hepatocytes in drug discovery and clinical studies, as well as, in 2D and 3D conformation and liver formation.

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Munther Alomari

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Alomari, M. (2021). Differentiation of Stem Cells into Hepatocyte Lineage: In Vitro Cell Culture, In Vivo Transplantation in Animal Models. In: Khan, F.A. (eds) Advances in Application of Stem Cells: From Bench to Clinics. Stem Cell Biology and Regenerative Medicine, vol 69. Humana, Cham. https://doi.org/10.1007/978-3-030-78101-9_6

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