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Human chorionic-plate-derived mesenchymal stem cells and Wharton’s jelly-derived mesenchymal stem cells: a comparative analysis of their potential as placenta-derived stem cells

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Abstract

Placenta-derived stem cells (PDSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal and differentiation and their immunomodulatory properties. Although many studies have characterized various PDSCs biologically, the properties of the self-renewal and differentiation potential among PDSCs have not yet been directly compared. We consider the characterization of chorionic-plate-derived mesenchymal stem cells (CP-MSCs) and Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) among various PDSCs and the assessment of their differentiation potential to be important for future studies into the applicability and effectiveness of PDSCs in cell therapy. In the present study, the capacities for self-renewal and multipotent differentiation of CP-MSCs and WJ-MSC isolated from normal term placentas were compared. CP-MSCs and WJ-MSCs expressed mRNAs for the pluripotent stem cell markers Oct-4, Nanog, and Sox-2. Additionally, HLA-G for immunomodulatory effects was found to be expressed at both the mRNA and protein levels in both cell types. The CP-MSCs and WJ-MSCs also had the capacities to differentiate into cells of mesodermal (adipogenic and osteogenic) and endodermal (hepatogenic) lineages. Expression of adipogenesis-related genes was higher in CP-MSCs than in WJ-MSCs, whereas WJ-MSCs accumulated more mineralized matrix than CP-MSCs. The WJ-MSCs expressed more of CYP3A4 mRNA, a marker for mature hepatocytes, than CP-MSCs. Thus, we propose that CP-MSCs and WJ-MSCs are useful sources of cells for appropriate clinical applications in the treatment of various degenerative diseases.

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Acknowledgments

We thank Dr. Chong Jai Kim (Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan, USA) for critical comments on the manuscript.

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Authors and Affiliations

  1. Department of Biomedical Science, CHA University, 606-16 Yeoksam1-dong, Gangnam-gu, Seoul, Republic of Korea, 135-081

    Mi Jeong Kim, Kyung Seon Shin, Jin Hee Jeon, Dong Ryul Lee, Sung Han Shim & Gi Jin Kim

  2. Graduate School of Life Science and Biotechnology, CHA University, Seoul, Republic of Korea

    Dong Ryul Lee

  3. Department of BioScience, CHA University, Sungnam, Republic of Korea

    Jin Kyeoung Kim

  4. Departments of Obstetrics and Gynecology, Gangnam CHA Medical Center, CHA University, Seoul, Republic of Korea

    Dong-Hyun Cha

  5. Fertility Center of CHA General Hospital, CHA Research Institute, CHA University, 606-4 Yeoksam1-dong, Gangnam-gu, Seoul, Republic of Korea, 135-081

    Tae Ki Yoon

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  1. Mi Jeong Kim
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  2. Kyung Seon Shin
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  3. Jin Hee Jeon
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  4. Dong Ryul Lee
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  6. Jin Kyeoung Kim
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  8. Tae Ki Yoon
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  9. Gi Jin Kim
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Corresponding authors

Correspondence to Tae Ki Yoon or Gi Jin Kim.

Additional information

This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A084923).

No conflicts of interest need to be declared by any of the authors.

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Kim, M.J., Shin, K.S., Jeon, J.H. et al. Human chorionic-plate-derived mesenchymal stem cells and Wharton’s jelly-derived mesenchymal stem cells: a comparative analysis of their potential as placenta-derived stem cells. Cell Tissue Res 346, 53–64 (2011). https://doi.org/10.1007/s00441-011-1249-8

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  • DOI: https://doi.org/10.1007/s00441-011-1249-8

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