Abstract
Cyanobacteria have two unique characteristics that distinguish them from all other prokaryotes – being able to perform oxygenic photosynthesis and having a circadian clock. Because cyanobacteria have a relatively simple genetic background and usually grow much faster than higher plants, they have become promising microbial hosts to produce biofuels and chemicals directly from sunlight and CO2. Recent advances in synthetic biology and systems biology not only have provided impetus for reprogramming metabolic networks of cyanobacterial host cells, but also have greatly expanded our knowledge of the genetics, physiology, and regulatory mechanisms of cyanobacteria. The plasticity of cyanobacterial central metabolism allows researchers to reprogram the metabolic networks to enhance the production of chemicals, but the metabolic regulation that prevents maximizing chemical production is still poorly understood. The cyanobacterial circadian clock exerts global gene regulations and oscillates intracellular metabolism. Manipulation of the circadian clock genes has proven to be an effective strategy for modulating the expression patterns of both endogenous and exogenous genes, driving either constant up- or down-regulation, which might be leveraged to enhance the production of biofuels and chemicals in cyanobacteria.
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References
Abernathy MH, Yu J, Ma F et al (2017) Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis. Biotechnol Biofuels 10(1):273. https://doi.org/10.1186/s13068-017-0958-y
Abernathy MH, Czajka JJ, Allen DK et al (2019) Cyanobacterial carboxysome mutant analysis reveals the influence of enzyme compartmentalization on cellular metabolism and metabolic network rigidity. Metab Eng 54:222–231. https://doi.org/10.1016/j.ymben.2019.04.010
Abramson BW, Kachel B, Kramer DM et al (2016) Increased photochemical efficiency in Cyanobacteria via an engineered sucrose sink. Plant Cell Physiol 57(12):2451–2460. https://doi.org/10.1093/pcp/pcw169
Aizouq M, Peisker H, Gutbrod K et al (2020) Triacylglycerol and phytyl ester synthesis in Synechocystis sp. PCC6803. Proc Nat Acad Sci 117(11):6216–6222. https://doi.org/10.1073/pnas.1915930117
Alagesan S, Gaudana SB, Sinha A et al (2013) Metabolic flux analysis of Cyanothece sp. ATCC 51142 under mixotrophic conditions. Photosynth Res 118(1-2):191–198. https://doi.org/10.1007/s11120-013-9911-5
Albers SC, Gallegos VA, Peebles CA (2015) Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. J Biotechnol 216:36–46. https://doi.org/10.1016/j.jbiotec.2015.09.042
Anfelt J, Kaczmarzyk D, Shabestary K et al (2015) Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production. Microbial Cell Factories 14(1):167. https://doi.org/10.1186/s12934-015-0355-9
Angermayr SA, Hellingwerf KJ (2013) On the use of metabolic control analysis in the optimization of cyanobacterial biosolar cell factories. J Phys Chem B 117(38):11169–11175. https://doi.org/10.1021/jp4013152
Angermayr SA, van der Woude AD, Correddu D et al (2014) Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803. Biotechnol Biofuels 7:99. https://doi.org/10.1186/1754-6834-7-99
Angermayr SA, Gorchs Rovira A, Hellingwerf KJ (2015) Metabolic engineering of cyanobacteria for the synthesis of commodity products. Trends Biotechnol 33(6):352–361. https://doi.org/10.1016/j.tibtech.2015.03.009
Appel J, Hueren V, Boehm M et al (2020) Cyanobacterial in vivo solar hydrogen production using a photosystem I–hydrogenase (PsaD-HoxYH) fusion complex. Nat Energy. https://doi.org/10.1038/s41560-020-0609-6
Atsumi S, Higashide W, Liao JC (2009) Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat Biotechnol 27(12):1177–1180. https://doi.org/10.1038/nbt.1586
Battchikova N, Muth-Pawlak D, Aro EM (2018) Proteomics of cyanobacteria: current horizons. Curr Opin Biotechnol 54:65–71. https://doi.org/10.1016/j.copbio.2018.02.012
Behle A, Saake P, Germann AT et al (2020) Comparative dose-response analysis of inducible promoters in cyanobacteria. ACS Synth Biol 9(4):843–855. https://doi.org/10.1021/acssynbio.9b00505
Behler J, Vijay D, Hess WR et al (2018) CRISPR-based technologies for metabolic engineering in cyanobacteria. Trends Biotechnol 36(10):996–1010. https://doi.org/10.1016/j.tibtech.2018.05.011
Bishé B, Taton A, Golden JW (2019) Modification of RSF1010-based broad-host-range plasmids for improved conjugation and cyanobacterial bioprospecting. iScience 20:216–228. https://doi.org/10.1016/j.isci.2019.09.002
Blankenship RE, Tiede DM, Barber J et al (2011) Comparing Photosynthetic and Photovoltaic Efficiencies and Recognizing the Potential for Improvement. Science 332(6031):805–809. https://doi.org/10.1126/science.1200165
Broddrick JT, Rubin BE, Welkie DG et al (2016) Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis. Proc Natl Acad Sci 113(51):E8344–E8353. https://doi.org/10.1073/pnas.1613446113
Cao YQ, Li Q, Xia PF et al (2017) AraBAD based toolkit for gene expression and metabolic robustness improvement in Synechococcus elongatus. Sci Rep 7(1):18059. https://doi.org/10.1038/s41598-017-17035-4
Carrieri D, Paddock T, Maness P-C et al (2012) Photo-catalytic conversion of carbon dioxide to organic acids by a recombinant cyanobacterium incapable of glycogen storage. Energy Environ Sci 5(11):9457–9461. https://doi.org/10.1039/C2EE23181F
Caspi R, Billington R, Ferrer L et al (2015) The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway/genome databases. Nucleic Acids Res 44(D1):D471–D480. https://doi.org/10.1093/nar/gkv1164
Cheah YE, Xu Y, Sacco SA et al (2020) Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942. Metab Eng 60:56–65. https://doi.org/10.1016/j.ymben.2020.03.007
Chen M, Blankenship RE (2011) Expanding the solar spectrum used by photosynthesis. Trends Plant Sci 16(8):427–431. https://doi.org/10.1016/j.tplants.2011.03.011
Chen M, Schliep M, Willows RD et al (2010) A red-shifted Chlorophyll. Science 329(5997):1318–1319. https://doi.org/10.1126/science.1191127
Chen Y, Taton A, Go M et al (2016) Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Microbiology (Reading, England) 162(12):2029–2041. https://doi.org/10.1099/mic.0.000377
Claassens NJ, Sousa DZ, dos Santos VAPM et al (2016) Harnessing the power of microbial autotrophy. Nat Rev Microbiol 14(11):692–706. https://doi.org/10.1038/nrmicro.2016.130
Cohen SE, Golden SS (2015) Circadian Rhythms in Cyanobacteria. Microbiol Mol Biol Rev 79(4):373–385. https://doi.org/10.1128/MMBR.00036-15
David C, Schmid A, Adrian L et al (2018) Production of 1,2-propanediol in photoautotrophic Synechocystis is linked to glycogen turn-over. Biotechnol Bioeng 115(2):300–311. https://doi.org/10.1002/bit.26468
Deng MD, Coleman JR (1999) Ethanol synthesis by genetic engineering in cyanobacteria. Appl Environ Microbiol 65(2):523–528
Diamond S, Jun D, Rubin BE et al (2015) The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth. Proc Natl Acad Sci 112(15):E1916–E1925. https://doi.org/10.1073/pnas.1504576112
Dong T, Wang B, Xiong W et al (2020) System-level optimization to improve biofuel potential via genetic engineering and hydrothermal liquefaction. ACS Sustain Chem Eng 8(7):2753–2762. https://doi.org/10.1021/acssuschemeng.9b06480
Douchi D, Liang F, Cano M et al (2019) Membrane-inlet mass spectrometry enables a quantitative understanding of inorganic carbon uptake flux and carbon concentrating mechanisms in metabolically engineered cyanobacteria. Front Microbiol 10:1356. https://doi.org/10.3389/fmicb.2019.01356
Du W, Liang F, Duan Y et al (2013) Exploring the photosynthetic production capacity of sucrose by cyanobacteria. Metab Eng 19:17–25. https://doi.org/10.1016/j.ymben.2013.05.001
Du W, Jongbloets JA, Guillaume M et al (2019) Exploiting day- and night-time metabolism of Synechocystis sp. PCC 6803 for fitness-coupled fumarate production around the clock. ACS Synth Biol 8(10):2263–2269. https://doi.org/10.1021/acssynbio.9b00289
Ducat DC, Silver PA (2012) Improving carbon fixation pathways. Curr Opin Chem Biol 16(3-4):337–344. https://doi.org/10.1016/j.cbpa.2012.05.002
Ducat DC, Avelar-Rivas JA, Way JC et al (2012) Rerouting carbon flux to enhance photosynthetic productivity. Appl Environ Microbiol 78(8):2660–2668. https://doi.org/10.1128/aem.07901-11
Durall C, Lindberg P, Yu J et al (2020) Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels 13:16. https://doi.org/10.1186/s13068-020-1653-y
Durao P, Aigner H, Nagy P et al (2015) Opposing effects of folding and assembly chaperones on evolvability of Rubisco. Nat Chem Biol 11(2):148–155. https://doi.org/10.1038/nchembio.1715
Englund E, Liang F, Lindberg P (2016) Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Sci Rep 6:36640. https://doi.org/10.1038/srep36640
Flores E, Arévalo S, Burnat M (2019) Cyanophycin and arginine metabolism in cyanobacteria. Algal Res 42:101577. https://doi.org/10.1016/j.algal.2019.101577
Gan F, Zhang S, Rockwell NC et al (2014) Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light. Science 345(6202):1312–1317. https://doi.org/10.1126/science.1256963
Gao Z, Zhao H, Li Z et al (2012) Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria. Energy Environ Sci 5(12):9857–9865. https://doi.org/10.1039/C2EE22675H
Gao X, Gao F, Liu D et al (2016a) Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO2. Energy Environ Sci 9(4):1400–1411. https://doi.org/10.1039/C5EE03102H
Gao X, Sun T, Pei G et al (2016b) Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals. Appl Microbiol Biotechnol 100(8):3401–3413. https://doi.org/10.1007/s00253-016-7374-2
Geerts D, Bovy A, de Vrieze G et al (1995) Inducible expression of heterologous genes targeted to a chromosomal platform in the cyanobacterium Synechococcus sp. PCC 7942. Microbiology 141(Pt 4):831–841. https://doi.org/10.1099/13500872-141-4-831
Godlewska K, Michalak I, Pacyga P et al (2019) Potential applications of cyanobacteria: Spirulina platensis filtrates and homogenates in agriculture. World J Microbiol Biotechnol 35(6):80–80. https://doi.org/10.1007/s11274-019-2653-6
Gorbunov MY, Kuzminov FI, Fadeev VV et al (2011) A kinetic model of non-photochemical quenching in cyanobacteria. Biochim Biophys Acta 1807(12):1591–1599. https://doi.org/10.1016/j.bbabio.2011.08.009
Gordon GC, Pfleger BF (2018) Regulatory tools for controlling gene expression in cyanobacteria. Adv Exp Med Biol 1080:281–315. https://doi.org/10.1007/978-981-13-0854-3_12
Gordon GC, Korosh TC, Cameron JC et al (2016) CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002. Metab Eng 38:170–179. https://doi.org/10.1016/j.ymben.2016.07.007
Grobbelaar N, Huang TC, Lin HY et al (1986) Dinitrogen-fixing endogenous rhythm in Synechococcus RF-1. FEMS Microbiol Lett 37(2):173–177
Guerrero F, Carbonell V, Cossu M et al (2012) Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One 7(11):e50470. https://doi.org/10.1371/journal.pone.0050470
Hamilton TL, Bryant DA, Macalady JL (2016) The role of biology in planetary evolution: cyanobacterial primary production in low-oxygen Proterozoic oceans. Environ Microbiol 18(2):325–340. https://doi.org/10.1111/1462-2920.13118
Hasunuma T, Matsuda M, Kondo A (2016) Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism. Metab Eng Commun 3:130–141. https://doi.org/10.1016/j.meteno.2016.04.003
Hellweger FL, Jabbur ML, Johnson CH et al (2020) Circadian clock helps cyanobacteria manage energy in coastal and high latitude ocean. ISME J 14(2):560–568. https://doi.org/10.1038/s41396-019-0547-0
Hendry JI, Bandyopadhyay A, Srinivasan S et al (2019) Metabolic model guided strain design of cyanobacteria. Curr Opin Biotechnol 64:17–23. https://doi.org/10.1016/j.copbio.2019.08.011
Higo A, Isu A, Fukaya Y et al (2016) Efficient gene induction and endogenous gene repression systems for the filamentous cyanobacterium Anabaena sp. PCC 7120. Plant Cell Physiol 57(2):387–396. https://doi.org/10.1093/pcp/pcv202
Hirokawa Y, Maki Y, Tatsuke T et al (2016) Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway. Metab Eng 34:97–103. https://doi.org/10.1016/j.ymben.2015.12.008
Hirokawa Y, Goto R, Umetani Y et al (2017a) Construction of a novel d-lactate producing pathway from dihydroxyacetone phosphate of the Calvin cycle in cyanobacterium, Synechococcus elongatus PCC 7942. J Biosci Bioeng 124(1):54–61. https://doi.org/10.1016/j.jbiosc.2017.02.016
Hirokawa Y, Matsuo S, Hamada H et al (2017b) Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution. Microb Cell Fact 16(1):212. https://doi.org/10.1186/s12934-017-0824-4
Hirokawa Y, Kubo T, Soma Y et al (2020) Enhancement of acetyl-CoA flux for photosynthetic chemical production by pyruvate dehydrogenase complex overexpression in Synechococcus elongatus PCC 7942. Metab Eng 57:23–30. https://doi.org/10.1016/j.ymben.2019.07.012
Hu G, Zhou J, Chen X et al (2018) Engineering synergetic CO2-fixing pathways for malate production. Metab Eng 47:496–504. https://doi.org/10.1016/j.ymben.2018.05.007
Huang HH, Camsund D, Lindblad P et al (2010) Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Res 38(8):2577–2593. https://doi.org/10.1093/nar/gkq164
Huo Y-X, Cho KM, Rivera JGL et al (2011) Conversion of proteins into biofuels by engineering nitrogen flux. Nat Biotechnol 29(4):346–351. https://doi.org/10.1038/nbt.1789
Immethun CM, DeLorenzo DM, Focht CM et al (2017) Physical, chemical, and metabolic state sensors expand the synthetic biology toolbox for Synechocystis sp. PCC 6803. Biotechnol Bioeng 114(7):1561–1569. https://doi.org/10.1002/bit.26275
Ito H, Mutsuda M, Murayama Y et al (2009) Cyanobacterial daily life with Kai-based circadian and diurnal genome-wide transcriptional control in Synechococcus elongatus. Proc Natl Acad Sci U S A 106(33):14168–14173. https://doi.org/10.1073/pnas.0902587106
Iwasaki H, Nishiwaki T, Kitayama Y et al (2002) KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria. Proc Natl Acad Sci U S A 99(24):15788–15793. https://doi.org/10.1073/pnas.222467299
Jacobsen JH, Frigaard NU (2014) Engineering of photosynthetic mannitol biosynthesis from CO2 in a cyanobacterium. Metab Eng 21:60–70. https://doi.org/10.1016/j.ymben.2013.11.004
Jaiswal D, Sengupta A, Sohoni S et al (2018) Genome features and biochemical characteristics of a robust, fast growing and naturally transformable cyanobacterium synechococcus elongatus PCC 11801 isolated from India. Sci Rep 8(1):16632. https://doi.org/10.1038/s41598-018-34872-z
Jazmin LJ, Xu Y, Cheah YE et al (2017) Isotopically nonstationary 13C flux analysis of cyanobacterial isobutyraldehyde production. Metab Eng 42:9–18. https://doi.org/10.1016/j.ymben.2017.05.001
Jin H, Wang Y, Idoine A et al (2018) Construction of a shuttle vector using an endogenous plasmid from the cyanobacterium Synechocystis sp. PCC6803. Front Microbiol 9:1662. https://doi.org/10.3389/fmicb.2018.01662
Jin H, Lindblad P, Bhaya D (2019) Building an Inducible T7 RNA Polymerase/T7 Promoter Circuit in Synechocystis sp. PCC6803. ACS Synth Biol 8(4):655–660. https://doi.org/10.1021/acssynbio.8b00515
Johnson CH, Zhao C, Xu Y et al (2017) Timing the day: what makes bacterial clocks tick? Nat Rev Microbiol 15(4):232–242. https://doi.org/10.1038/nrmicro.2016.196
Kamennaya NA, Ahn S, Park H et al (2015) Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production. Metab Eng 29:76–85. https://doi.org/10.1016/j.ymben.2015.03.002
Kamravamanesh D, Kiesenhofer D, Fluch S et al (2019) Scale-up challenges and requirement of technology-transfer for cyanobacterial poly (3-hydroxybutyrate) production in industrial scale. Int J Biobased Plastics 1(1):60–71. https://doi.org/10.1080/24759651.2019.1688604
Kanehisa M, Sato Y, Furumichi M et al (2019) New approach for understanding genome variations in KEGG. Nucleic Acids Res 47(D1):D590–D595. https://doi.org/10.1093/nar/gky962
Kaneko T, Sato S, Kotani H et al (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res 3(3):109–136. https://doi.org/10.1093/dnares/3.3.109
Kaneko T, Nakamura Y, Wolk CP et al (2001) Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res 8(5):205–213.; 227–253. https://doi.org/10.1093/dnares/8.5.205
Kasting JF, Siefert JL (2002) Life and the Evolution of Earth’s Atmosphere. Science 296(5570):1066–1068. https://doi.org/10.1126/science.1071184
Kelly CL, Taylor GM, Hitchcock A et al (2018) A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria. ACS Synth Biol 7(4):1056–1066. https://doi.org/10.1021/acssynbio.7b00435
Kirsch F, Klahn S, Hagemann M (2019) Salt-Regulated Accumulation of the Compatible Solutes Sucrose and Glucosylglycerol in Cyanobacteria and Its Biotechnological Potential. Front Microbiol 10:2139. https://doi.org/10.3389/fmicb.2019.02139
Kirst H, Formighieri C, Melis A (2014) Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size. Biochim Biophys Acta 1837(10):1653–1664. https://doi.org/10.1016/j.bbabio.2014.07.009
Klahn S, Hagemann M (2011) Compatible solute biosynthesis in cyanobacteria. Environ Microbiol 13(3):551–562. https://doi.org/10.1111/j.1462-2920.2010.02366.x
Klodawska K, Bujas A, Turos-Cabal M et al (2019) Effect of growth temperature on biosynthesis and accumulation of carotenoids in cyanobacterium Anabaena sp. PCC 7120 under diazotrophic conditions. Microbiol Res 226:34–40. https://doi.org/10.1016/j.micres.2019.05.003
Knoot CJ, Ungerer J, Wangikar PP et al (2018) Cyanobacteria: Promising biocatalysts for sustainable chemical production. J Biol Chem 293(14):5044–5052. https://doi.org/10.1074/jbc.R117.815886
Kolman MA, Nishi CN, Perez-Cenci M et al (2015) Sucrose in cyanobacteria: from a salt-response molecule to play a key role in nitrogen fixation. Life (Basel) 5(1):102–126. https://doi.org/10.3390/life5010102
Kondo T, Strayer CA, Kulkarni RD et al (1993) Circadian rhythms in prokaryotes: luciferase as a reporter of circadian gene expression in cyanobacteria. Proc Natl Acad Sci U S A 90(12):5672–5676. https://doi.org/10.1073/pnas.90.12.5672
Korosh TC, Markley AL, Clark RL et al (2017) Engineering photosynthetic production of L-lysine. Metab Eng 44:273–283. https://doi.org/10.1016/j.ymben.2017.10.010
Ku JT, Lan EI (2018) A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO2. Metab Eng 46:35–42. https://doi.org/10.1016/j.ymben.2018.02.004
Kuan D, Duff S, Posarac D et al (2015) Growth optimization of Synechococcus elongatus PCC7942 in lab flasks and a 2-D photobioreactor. Can J Chem Eng 93(4):640–647. https://doi.org/10.1002/cjce.22154
Lai MC, Lan EI (2015) Advances in Metabolic Engineering of Cyanobacteria for Photosynthetic Biochemical Production. Metabolites 5(4):636–658. https://doi.org/10.3390/metabo5040636
Lan EI, Wei CT (2016) Metabolic engineering of cyanobacteria for the photosynthetic production of succinate. Metab Eng 38:483–493. https://doi.org/10.1016/j.ymben.2016.10.014
Lan EI, Ro SY, Liao JC (2013) Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria. Energy Environ Sci 6(9):2672–2681. https://doi.org/10.1039/C3EE41405A
Lan EI, Chuang DS, Shen CR et al (2015) Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942. Metab Eng 31:163–170. https://doi.org/10.1016/j.ymben.2015.08.002
Lee HJ, Son J, Sim SJ et al (2020) Metabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria. Plant Biotechnol J. https://doi.org/10.1111/pbi.13342
Li H, Liao JC (2013) Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol. Microb Cell Fact 12(1):4. https://doi.org/10.1186/1475-2859-12-4
Li C, Tao F, Ni J et al (2015) Enhancing the light-driven production of D-lactate by engineering cyanobacterium using a combinational strategy. Sci Rep 5:9777. https://doi.org/10.1038/srep09777
Li H, Shen CR, Huang C-H et al (2016) CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production. Metab Eng 38:293–302. https://doi.org/10.1016/j.ymben.2016.09.006
Liang F, Lindblad P (2016) Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803. Metab Eng 38:56–64. https://doi.org/10.1016/j.ymben.2016.06.005
Liang F, Englund E, Lindberg P et al (2018a) Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio. Metab Eng 46:51–59. https://doi.org/10.1016/j.ymben.2018.02.006
Liang F, Lindberg P, Lindblad P (2018b) Engineering photoautotrophic carbon fixation for enhanced growth and productivity. Sustain Energy Fuels 2(12):2583–2600. https://doi.org/10.1039/C8SE00281A
Liberton M, Bandyopadhyay A, Pakrasi HB (2019) Enhanced Nitrogen Fixation in a glgX-Deficient Strain of Cyanothece sp. Strain ATCC 51142, a Unicellular Nitrogen-Fixing Cyanobacterium. Appl Environ Microbiol 85(7):e02887-18. https://doi.org/10.1128/AEM.02887-18
Lin P-C, Pakrasi HB (2019) Engineering cyanobacteria for production of terpenoids. Planta 249(1):145–154. https://doi.org/10.1007/s00425-018-3047-y
Lin MT, Occhialini A, Andralojc PJ et al (2014) A faster Rubisco with potential to increase photosynthesis in crops. Nature 513(7519):547–550. https://doi.org/10.1038/nature13776
Lin PC, Zhang F, Pakrasi HB (2020) Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep 10(1):390. https://doi.org/10.1038/s41598-019-57319-5
Lindberg P, Park S, Melis A (2010) Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng 12(1):70–79. https://doi.org/10.1016/j.ymben.2009.10.001
Liu X, Curtiss R 3rd (2009) Nickel-inducible lysis system in Synechocystis sp. PCC 6803. Proc Natl Acad Sci U S A 106(51):21550–21554. https://doi.org/10.1073/pnas.0911953106
Liu D, Pakrasi HB (2018) Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803. Microbial Cell Factories 17(1):48. https://doi.org/10.1186/s12934-018-0897-8
Liu Y, Tsinoremas NF, Johnson CH et al (1995) Circadian orchestration of gene expression in cyanobacteria. Genes Dev 9(12):1469–1478. https://doi.org/10.1101/gad.9.12.1469
Liu X, Sheng J, Curtiss R 3rd (2011) Fatty acid production in genetically modified cyanobacteria. Proc Natl Acad Sci U S A 108(17):6899–6904. https://doi.org/10.1073/pnas.1103014108
Liu X, Miao R, Lindberg P et al (2019) Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria. Energy Environ Sci 12(9):2765–2777. https://doi.org/10.1039/C9EE01214A
Luan G, Lu X (2018) Tailoring cyanobacterial cell factory for improved industrial properties. Biotechnol Adv 36(2):430–442. https://doi.org/10.1016/j.biotechadv.2018.01.005
Luan G, Zhang S, Lu X (2020) Engineering cyanobacteria chassis cells toward more efficient photosynthesis. Curr Opin Biotechnol 62:1–6. https://doi.org/10.1016/j.copbio.2019.07.004
Ludwig M, Bryant DA (2012) Synechococcus sp. strain PCC 7002 transcriptome: acclimation to temperature, salinity, oxidative stress, and mixotrophic growth conditions. Front Microbiol 3:354. https://doi.org/10.3389/fmicb.2012.00354
Ma AT, Schmidt CM, Golden JW (2014) Regulation of gene expression in diverse cyanobacterial species by using theophylline-responsive riboswitches. Appl Environ Microbiol 80(21):6704–6713. https://doi.org/10.1128/aem.01697-14
Markham JN, Tao L, Davis R et al (2016) Techno-economic analysis of a conceptual biofuel production process from bioethylene produced by photosynthetic recombinant cyanobacteria. Green Chem 18(23):6266–6281. https://doi.org/10.1039/C6GC01083K
Markley AL, Begemann MB, Clarke RE et al (2015) Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. ACS Synth Biol 4(5):595–603. https://doi.org/10.1021/sb500260k
Melis A (2009) Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency. Plant Sci 177(4):272–280. https://doi.org/10.1016/j.plantsci.2009.06.005
Misumi M, Katoh H, Tomo T et al (2016) Relationship between photochemical quenching and non-photochemical quenching in six species of cyanobacteria reveals species difference in redox state and species commonality in energy dissipation. Plant Cell Physiol 57(7):1510–1517. https://doi.org/10.1093/pcp/pcv185
Mitschke J, Georg J, Scholz I et al (2011) An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803. Proc Natl Acad Sci U S A 108(5):2124–2129. https://doi.org/10.1073/pnas.1015154108
Moser S, Pichler H (2019) Identifying and engineering the ideal microbial terpenoid production host. Appl Microbiol Biotechnol 103(14):5501–5516. https://doi.org/10.1007/s00253-019-09892-y
Nakahira Y, Ogawa A, Asano H et al (2013) Theophylline-dependent riboswitch as a novel genetic tool for strict regulation of protein expression in cyanobacterium Synechococcus elongatus PCC 7942. Plant Cell Physiol 54(10):1724–1735. https://doi.org/10.1093/pcp/pct115
Nakajima T, Yoshikawa K, Toya Y et al (2017) Metabolic flux analysis of the Synechocystis sp. PCC 6803 DeltanrtABCD mutant reveals a mechanism for metabolic adaptation to nitrogen-limited conditions. Plant Cell Physiol 58(3):537–545. https://doi.org/10.1093/pcp/pcw233
Niederholtmeyer H, Wolfstadter BT, Savage DF et al (2010) Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol 76(11):3462–3466. https://doi.org/10.1128/AEM.00202-10
Niu T-C, Lin G-M, Xie L-R et al (2019) Expanding the potential of CRISPR-Cpf1-based genome editing technology in the cyanobacterium Anabaena PCC 7120. ACS Synthetic Biol 8(1):170–180. https://doi.org/10.1021/acssynbio.8b00437
Nudler E, Mironov AS (2004) The riboswitch control of bacterial metabolism. Trends Biochem Sci 29(1):11–17. https://doi.org/10.1016/j.tibs.2003.11.004
Nurnberg DJ, Morton J, Santabarbara S et al (2018) Photochemistry beyond the red limit in chlorophyll f-containing photosystems. Science 360(6394):1210–1213. https://doi.org/10.1126/science.aar8313
Oliver JW, Machado IM, Yoneda H et al (2013) Cyanobacterial conversion of carbon dioxide to 2,3-butanediol. Proc Natl Acad Sci U S A 110(4):1249–1254. https://doi.org/10.1073/pnas.1213024110
Ouyang Y, Andersson CR, Kondo T et al (1998) Resonating circadian clocks enhance fitness in cyanobacteria. Proc Natl Acad Sci U S A 95(15):8660–8664. https://doi.org/10.1073/pnas.95.15.8660
Park J, Choi Y (2017) Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: a mini review. Front Chem Sci Eng 11(1):66–71. https://doi.org/10.1007/s11705-016-1591-1
Pattanayak GK, Phong C, Rust MJ (2014) Rhythms in energy storage control the ability of the cyanobacterial circadian clock to reset. Curr Biol 24(16):1934–1938. https://doi.org/10.1016/j.cub.2014.07.022
Pérez AA, Gajewski JP, Ferlez BH et al (2017) Zn2+-inducible expression platform for Synechococcus sp. strain PCC 7002 based on the smtA promoter/operator and smtB repressor. Appl Environ Microbiol 83(3):e02491–e02416. https://doi.org/10.1128/aem.02491-16
Peterhansel C, Krause K, Braun H-P et al (2013) Engineering photorespiration: current state and future possibilities. Plant Biol 15(4):754–758. https://doi.org/10.1111/j.1438-8677.2012.00681.x
Placzek S, Schomburg I, Chang A et al (2016) BRENDA in 2017: new perspectives and new tools in BRENDA. Nucleic Acids Res 45(D1):D380–D388. https://doi.org/10.1093/nar/gkw952
Qiao C, Duan Y, Zhang M et al (2018) Effects of reduced and enhanced glycogen pools on salt-induced sucrose production in a sucrose-secreting strain of Synechococcus elongatus PCC 7942. Appl Environ Microbiol 84(2):e02023–e02017. https://doi.org/10.1128/aem.02023-17
Ruffing AM (2014) Improved Free Fatty Acid Production in Cyanobacteria with Synechococcus sp. PCC 7002 as Host. Front Bioeng Biotechnol 2:17. https://doi.org/10.3389/fbioe.2014.00017
Ruffing AM, Jensen TJ, Strickland LM (2016) Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis. Microbial Cell Factories 15(1):190. https://doi.org/10.1186/s12934-016-0584-6
Sakai M, Ogawa T, Matsuoka M et al (1997) Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae. J Ferment Bioeng 84(5):434–443. https://doi.org/10.1016/S0922-338X(97)82004-1
Sake CL, Metcalf AJ, Boyle NR (2019) The challenge and potential of photosynthesis: unique considerations for metabolic flux measurements in photosynthetic microorganisms. Biotechnol Lett 41(1):35–45. https://doi.org/10.1007/s10529-018-2622-4
Salis HM, Mirsky EA, Voigt CA (2009) Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol 27(10):946–950. https://doi.org/10.1038/nbt.1568
Santos-Merino M, Singh AK, Ducat DC (2019) New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering. Front Bioeng Biotechnol 7:33. https://doi.org/10.3389/fbioe.2019.00033
Saper G, Kallmann D, Conzuelo F et al (2018) Live cyanobacteria produce photocurrent and hydrogen using both the respiratory and photosynthetic systems. Nat Commun 9(1):2168. https://doi.org/10.1038/s41467-018-04613-x
Satagopan S, Huening KA, Tabita FR (2019) Selection of Cyanobacterial (Synechococcus sp. Strain PCC 6301) RubisCO variants with improved functional properties that confer enhanced CO2-dependent growth of Rhodobacter capsulatus, a Photosynthetic Bacterium. mBio 10(4):e01537-01519. https://doi.org/10.1128/mBio.01537-19
Savakis PE, Angermayr SA, Hellingwerf KJ (2013) Synthesis of 2,3-butanediol by Synechocystis sp. PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria. Metab Eng 20:121–130. https://doi.org/10.1016/j.ymben.2013.09.008
Savakis P, Tan X, Du W et al (2015) Photosynthetic production of glycerol by a recombinant cyanobacterium. J Biotechnol 195:46–51. https://doi.org/10.1016/j.jbiotec.2014.12.015
Schwarz D, Orf I, Kopka J et al (2013) Recent applications of metabolomics toward cyanobacteria. Metabolites 3(1):72–100. https://doi.org/10.3390/metabo3010072
Sebesta J, Peebles CAM (2020) Improving heterologous protein expression in Synechocystis sp. PCC 6803 for alpha-bisabolene production. Metab Eng Commun 10:e00117. https://doi.org/10.1016/j.mec.2019.e00117
Sengupta A, Pakrasi HB, Wangikar PP (2018) Recent advances in synthetic biology of cyanobacteria. Appl Microbiol Biotechnol 102(13):5457–5471. https://doi.org/10.1007/s00253-018-9046-x
Sengupta A, Sunder AV, Sohoni SV et al (2019) Fine-tuning native promoters of Synechococcus elongatus PCC 7942 to develop a synthetic toolbox for heterologous protein expression. ACS Synth Biol 8(5):1219–1223. https://doi.org/10.1021/acssynbio.9b00066
Shen CR, Liao JC (2012) Photosynthetic production of 2-methyl-1-butanol from CO2 in cyanobacterium Synechococcus elongatus PCC7942 and characterization of the native acetohydroxyacid synthase. Energy Environ Sci 5(11):9574–9583. https://doi.org/10.1039/C2EE23148D
Song K, Tan X, Liang Y et al (2016) The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production. Appl Microbiol Biotechnol 100(18):7865–7875. https://doi.org/10.1007/s00253-016-7510-z
Stockel J, Welsh EA, Liberton M et al (2008) Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes. Proc Natl Acad Sci U S A 105(16):6156–6161. https://doi.org/10.1073/pnas.0711068105
Sun T, Li S, Song X et al (2018) Toolboxes for cyanobacteria: Recent advances and future direction. Biotechnol Adv 36(4):1293–1307. https://doi.org/10.1016/j.biotechadv.2018.04.007
Swan JA, Golden SS, LiWang A et al (2018) Structure, function, and mechanism of the core circadian clock in cyanobacteria. J Biol Chem 293(14):5026–5034. https://doi.org/10.1074/jbc.TM117.001433
Takahama K, Matsuoka M, Nagahama K et al (2003) Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus. J Biosci Bioeng 95(3):302–305. https://doi.org/10.1016/S1389-1723(03)80034-8
Tan X, Yao L, Gao Q et al (2011) Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria. Metab Eng 13(2):169–176. https://doi.org/10.1016/j.ymben.2011.01.001
Tang K-H, Feng X, Bandyopadhyay A et al (2013) Unique central carbon metabolic pathways and novel enzymes in phototrophic bacteria revealed by integrative genomics, 13C-based metabolomics and fluxomics. In: Kuang T, Lu C, Zhang L (eds) Photosynthesis research for food, fuel and the future. Springer, Berlin, Heidelberg, pp 339–343
Taton A, et al (2020) The circadian clock and darkness control natural competence in cyanobacteria. Nat Commun 11:1688. https://doi.org/10.1038/s41467-020-15384-9
Thiel K, Mulaku E, Dandapani H et al (2018) Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803. Microbial Cell Factories 17(1):34. https://doi.org/10.1186/s12934-018-0882-2
Toepel J, Welsh E, Summerfield TC et al (2008) Differential transcriptional analysis of the cyanobacterium Cyanothece sp. strain ATCC 51142 during light-dark and continuous-light growth. J Bacteriol 190(11):3904–3913. https://doi.org/10.1128/JB.00206-08
Tschörtner J, Lai B, Krömer JO (2019) Biophotovoltaics: Green Power Generation From Sunlight and Water. Front Microbiol 10:866. https://doi.org/10.3389/fmicb.2019.00866
Ungerer J, Pakrasi HB (2016) Cpf1 is a versatile tool for CRISPR genome editing across diverse species of cyanobacteria. Sci Rep 6(1):39681. https://doi.org/10.1038/srep39681
Ungerer J, Tao L, Davis M et al (2012) Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803. Energy Environ Sci 5(10):8998–9006. https://doi.org/10.1039/C2EE22555G
Ungerer J, Lin PC, Chen HY et al (2018a) Adjustments to photosystem stoichiometry and electron transfer proteins are key to the remarkably fast growth of the cyanobacterium synechococcus elongatus UTEX 2973. mBio 9(1):e02327-17. https://doi.org/10.1128/mBio.02327-17
Ungerer J, Wendt KE, Hendry JI et al (2018b) Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973. Proc Natl Acad Sci U S A 115(50):E11761–E11770. https://doi.org/10.1073/pnas.1814912115
Ungerer J, Wendt KE, Hendry JI et al (2019) Reply to Zhou and Li: Plasticity of the genomic haplotype of Synechococcus elongatus leads to rapid strain adaptation under laboratory conditions. Proc Natl Acad Sci 116(10):3946–3947. https://doi.org/10.1073/pnas.1900792116
van Alphen P, Hellingwerf KJ (2015) Sustained circadian rhythms in continuous light in Synechocystis sp. pcc6803 growing in a well-controlled photobioreactor. PLoS One 10(6):e0127715. https://doi.org/10.1371/journal.pone.0127715
van Alphen P, Abedini Najafabadi H, Branco Dos Santos F et al (2018) Increasing the photoautotrophic growth rate of Synechocystis sp. PCC 6803 by identifying the limitations of its cultivation. Biotechnol J 13(8):e1700764. https://doi.org/10.1002/biot.201700764
van der Woude AD, Perez Gallego R, Vreugdenhil A et al (2016) Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol. Microb Cell Fact 15:60. https://doi.org/10.1186/s12934-016-0458-y
Varman AM, Yu Y, You L et al (2013) Photoautotrophic production of D-lactic acid in an engineered cyanobacterium. Microb Cell Fact 12:117. https://doi.org/10.1186/1475-2859-12-117
Vijayan V, Zuzow R, O'Shea EK (2009) Oscillations in supercoiling drive circadian gene expression in cyanobacteria. Proc Natl Acad Sci U S A 106(52):22564–22568. https://doi.org/10.1073/pnas.0912673106
Vu TT, Stolyar SM, Pinchuk GE et al (2012) Genome-scale modeling of light-driven reductant partitioning and carbon fluxes in diazotrophic unicellular cyanobacterium Cyanothece sp. ATCC 51142. PLoS Comput Biol 8(4):e1002460. https://doi.org/10.1371/journal.pcbi.1002460
Wang B, Wang J, Zhang W et al (2012) Application of synthetic biology in cyanobacteria and algae. Front Microbiol 3:344. https://doi.org/10.3389/fmicb.2012.00344
Wang B, Pugh S, Nielsen DR et al (2013a) Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2. Metab Eng 16:68–77. https://doi.org/10.1016/j.ymben.2013.01.001
Wang W, Liu X, Lu X (2013b) Engineering cyanobacteria to improve photosynthetic production of alka(e)nes. Biotechnol Biofuels 6(1):69. https://doi.org/10.1186/1754-6834-6-69
Wang Y, Tao F, Ni J et al (2015) Production of C3 platform chemicals from CO2 by genetically engineered cyanobacteria. Green Chem 17:3100–3110. https://doi.org/10.1039/C5GC00129C
Wang Y, Sun T, Gao X et al (2016) Biosynthesis of platform chemical 3-hydroxypropionic acid (3-HP) directly from CO2 in cyanobacterium Synechocystis sp. PCC 6803. Metab Eng 34:60–70. https://doi.org/10.1016/j.ymben.2015.10.008
Wang B, Eckert C, Maness PC et al (2018a) A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803. ACS Synth Biol 7(1):276–286. https://doi.org/10.1021/acssynbio.7b00297
Wang B, Xiong W, Yu J et al (2018b) Unlocking the photobiological conversion of CO2 to (R)-3-hydroxybutyrate in cyanobacteria. Green Chem 20(16):3772–3782. https://doi.org/10.1039/C8GC01208C
Wang B, Dong T, Myrlie A et al (2019) Photosynthetic production of the nitrogen-rich compound guanidine. Green Chemistry 21(11):2928–2937. https://doi.org/10.1039/C9GC01003C
Welsh EA, Liberton M, Stockel J et al (2008) The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle. Proc Natl Acad Sci U S A 105(39):15094–15099. https://doi.org/10.1073/pnas.0805418105
Wendt KE, Ungerer J, Cobb RE et al (2016) CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973. Microb Cell Fact 15(1):115. https://doi.org/10.1186/s12934-016-0514-7
Weyer KM, Bush DR, Darzins A et al (2010) Theoretical maximum Algal oil production. BioEnergy Res 3(2):204–213. https://doi.org/10.1007/s12155-009-9046-x
Wiegard A, Dorrich AK, Deinzer HT et al (2013) Biochemical analysis of three putative KaiC clock proteins from Synechocystis sp. PCC 6803 suggests their functional divergence. Microbiology 159(Pt 5):948–958. https://doi.org/10.1099/mic.0.065425-0
Wilson RH, Whitney SM (2017) Improving CO2 fixation by Enhancing Rubisco performance. In: Alcalde M (ed) Directed enzyme evolution: advances and applications. Springer International Publishing, Cham, pp 101–126. https://doi.org/10.1007/978-3-319-50413-1_4
Włodarczyk A, Selão TT, Norling B et al (2020) Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production. Commun Biol 3(1):215. https://doi.org/10.1038/s42003-020-0910-8
Woelfle MA, Ouyang Y, Phanvijhitsiri K et al (2004) The adaptive value of circadian clocks: an experimental assessment in cyanobacteria. Curr Biol 14(16):1481–1486. https://doi.org/10.1016/j.cub.2004.08.023
Xiong W, Morgan JA, Ungerer J et al (2015) The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene. Nature Plants 1(5):15053. https://doi.org/10.1038/nplants.2015.53
Xiong W, Cano M, Wang B et al (2017) The plasticity of cyanobacterial carbon metabolism. Curr Opin Chem Biol 41:12–19. https://doi.org/10.1016/j.cbpa.2017.09.004
Xu Y, Mori T, Johnson CH (2003) Cyanobacterial circadian clockwork: roles of KaiA, KaiB and the kaiBC promoter in regulating KaiC. EMBO J 22(9):2117–2126. https://doi.org/10.1093/emboj/cdg168
Xu Y, Alvey RM, Byrne PO et al (2011) Expression of genes in cyanobacteria: adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002. Methods Mol Biol 684:273–293. https://doi.org/10.1007/978-1-60761-925-3_21
Xu Y, Weyman PD, Umetani M et al (2013) Circadian yin-yang regulation and its manipulation to globally reprogram gene expression. Current Biol: CB 23(23):2365–2374. https://doi.org/10.1016/j.cub.2013.10.011
Xue Y, Zhang Y, Cheng D et al (2014) Genetically engineering Synechocystis sp. Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p-coumaric acid. Proc Natl Acad Sci U S A 111(26):9449–9454. https://doi.org/10.1073/pnas.1323725111
You L, Berla B, He L et al (2014) 13C-MFA delineates the photomixotrophic metabolism of Synechocystis sp. PCC 6803 under light- and carbon-sufficient conditions. Biotechnol J 9(5):684–692. https://doi.org/10.1002/biot.201300477
Young JD, Shastri AA, Stephanopoulos G et al (2011) Mapping photoautotrophic metabolism with isotopically nonstationary (13)C flux analysis. Metab Eng 13(6):656–665. https://doi.org/10.1016/j.ymben.2011.08.002
Yu King Hing N, Liang F, Lindblad P et al (2019) Combining isotopically non-stationary metabolic flux analysis with proteomics to unravel the regulation of the Calvin-Benson-Bassham cycle in Synechocystis sp. PCC 6803. Metab Eng 56:77–84. https://doi.org/10.1016/j.ymben.2019.08.014
Yu J, Liberton M, Cliften PF et al (2015) Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO(2). Sci Rep 5:8132. https://doi.org/10.1038/srep08132
Yu H, Li X, Duchoud F et al (2018) Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway. Nature Communications 9(1):2008. https://doi.org/10.1038/s41467-018-04417-z
Yunus IS, Jones PR (2018) Photosynthesis-dependent biosynthesis of medium chain-length fatty acids and alcohols. Metab Eng 49:59–68. https://doi.org/10.1016/j.ymben.2018.07.015
Zarzycki J, Axen SD, Kinney JN et al (2012) Cyanobacterial-based approaches to improving photosynthesis in plants. J Exp Bot 64(3):787–798. https://doi.org/10.1093/jxb/ers294
Zavřel T, Sinetova MA, Búzová D et al (2015) Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor. Eng Life Sci 15(1):122–132. https://doi.org/10.1002/elsc.201300165
Zehr JP (2011) Nitrogen fixation by marine cyanobacteria. Trends Microbiol 19(4):162–173. https://doi.org/10.1016/j.tim.2010.12.004
Zess EK, Begemann MB, Pfleger BF (2016) Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Biotechnol Bioeng 113(2):424–432. https://doi.org/10.1002/bit.25713
Zhang H, Liu Y, Nie X et al (2018) The cyanobacterial ornithine-ammonia cycle involves an arginine dihydrolase. Nat Chem Biol 14(6):575–581. https://doi.org/10.1038/s41589-018-0038-z
Zhou J, Zhang H, Zhang Y et al (2012) Designing and creating a modularized synthetic pathway in cyanobacterium Synechocystis enables production of acetone from carbon dioxide. Metab Eng 14(4):394–400. https://doi.org/10.1016/j.ymben.2012.03.005
Zhou J, Zhang H, Meng H et al (2014) Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep 4:4500. https://doi.org/10.1038/srep04500
Zhou J, Zhang F, Meng H et al (2016a) Introducing extra NADPH consumption ability significantly increases the photosynthetic efficiency and biomass production of cyanobacteria. Metab Eng 38:217–227. https://doi.org/10.1016/j.ymben.2016.08.002
Zhou J, Zhu T, Cai Z et al (2016b) From cyanochemicals to cyanofactories: a review and perspective. Microbial Cell Factories 15(1):2. https://doi.org/10.1186/s12934-015-0405-3
Zhu T, Xie X, Li Z et al (2015) Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803. Green Chem 17(1):421–434. https://doi.org/10.1039/C4GC01730G
Acknowledgments
The authors would like to thank Dr. Carl Hirschie Johnson for comments on the manuscript. This material is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science Program under award numbers DE-SC0019404 and DE-SC0019388, and NIH/NIGMS awards GM 067152 & GM 107434.
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Wang, B., Young, J.D., Xu, Y. (2021). Reprogramming Metabolic Networks and Manipulating Circadian Clocks for Biotechnological Applications. In: Johnson, C.H., Rust, M.J. (eds) Circadian Rhythms in Bacteria and Microbiomes. Springer, Cham. https://doi.org/10.1007/978-3-030-72158-9_14
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