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Twenty-First Century FISH: Focus on Interphase Chromosomes

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Human Interphase Chromosomes

Abstract

Interphase molecular cytogenetics provides opportunities for analysis of chromosomes in almost all types of human cells at any stage of the cell cycle. Generally, interphase fluorescence in situ hybridization (I-FISH) is a basic technological platform for visualization of individual chromosomes (chromosomal regions) in single cells. The achievements of studying human interphase chromosomes have allowed numerous discoveries in chromosome research (molecular cytogenetics) and genomics (cytogenomics). In the postgenomic era, interphase chromosome analysis by I-FISH remains an important part of biomedical research. Here, we describe the spectrum of FISH applications with special emphasis on interphase chromosome biology and molecular cytogenetic/cytogenomic diagnosis.

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Acknowledgments

We would like to express our gratitude to Dr. MA Zelenova for help in chapter preparation. Prof. SG Vorsanova, Dr. OS Kurinnaia, and Prof. IY Iourov are partially supported by RFBR and CITMA according to the research project No. 18-515-34005. Prof. IY Iourov’s lab is supported by the Government Assignment of the Russian Ministry of Science and Higher Education, Assignment no. AAAA-A19-119040490101-6. Prof. SG Vorsanova’s lab is supported by the Government Assignment of the Russian Ministry of Health, Assignment no. AAAA-A18-118051590122-7.

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Authors and Affiliations

  1. Veltischev Research and Clinical Institute for Pediatrics, Pirogov Russian National Research Medical University, Ministry of Health of Russian Federation, Moscow, Russia

    Svetlana G. Vorsanova, Yuri B. Yurov, Oxana S. Kurinnaia & Alexei D. Kolotii

  2. Mental Health Research Center, Russian Ministry of Science and Higher Education, Moscow, Russia

    Svetlana G. Vorsanova, Yuri B. Yurov, Oxana S. Kurinnaia & Alexei D. Kolotii

  3. Mental Health Research Center, Moscow, Russia

    Ivan Y. Iourov

  4. Veltischev Research and Clinical Institute for Pediatrics of the Pirogov Russian National Research Medical University, Ministry of Health of Russian Federation, Moscow, Russia

    Ivan Y. Iourov

  5. Medical Genetics Department of Russian Medical Academy of Continuous Postgraduate Education, Moscow, Russia

    Ivan Y. Iourov

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  1. Svetlana G. Vorsanova
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  2. Yuri B. Yurov
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  3. Oxana S. Kurinnaia
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  4. Alexei D. Kolotii
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  5. Ivan Y. Iourov
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Editors and Affiliations

  1. Mental Health Research Center, Russian Academy of Medical Sciences, Moscow, Russia

    Ivan Iourov

  2. Veltischev Research and Clinical Institute, Pirogov Russian National Research Medical University, Moscow, Russia

    Svetlana Vorsanova

  3. Mental Health Research Center, Russian Academy of Medical Sciences, Moscow, Russia

    Yuri Yurov

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Vorsanova, S.G., Yurov, Y.B., Kurinnaia, O.S., Kolotii, A.D., Iourov, I.Y. (2020). Twenty-First Century FISH: Focus on Interphase Chromosomes. In: Iourov, I., Vorsanova, S., Yurov, Y. (eds) Human Interphase Chromosomes. Springer, Cham. https://doi.org/10.1007/978-3-030-62532-0_7

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