Abstract
The imperative role of functionalized natural alginate in immobilization of Lactobacillus delbrucekii (NCIM 2365) cells in production of optically pure l (+) lactic acid was studied. L. delbrucekii cells were immobilized in alginate, succinylated alginate and carrageenan to evaluate the bead stability and selectivity towards production of optically pure l (+) lactic acid. The scanning electron microscopic studies of free and immobilized cells show little morphological changes. The present study highlights the use of functionalized alginate-immobilized L. delbrucekii cells in production of l (+) lactic acid in higher yields (0.93 Yp/s in grams) with an improved enantioselectivity (99%). In addition, they further revealed decreased by-product formation (acetic and propionic acid) when compared to free and other immobilized cell fermentation.
Similar content being viewed by others
References
Bulut, S., Elibol, M., & Ozer, D. (2004). Biochemical Engineering Journal, 21, 33–37.
Agouridis, N., Bekatorou, A., Nigam, P., & Kanellaki, M. (2005). Journal of Agricultural and Food Chemistry, 53, 2546–2551.
Guoqiang, D., Kaul, R., & Mattiasson, B. (1991). Applied Microbiology and Biotechnology, 36, 309–314.
Audet, P., Paquin, C., & Lacroix, C. (1988). Applied Microbiology and Biotechnology, 29, 11–18.
Goncalves, L. M. D., Barreto, M. T. O., Xavier, A. M. B. R., Carrondo, M. J. T., & Klein, J. (1992). Applied Microbiology and Biotechnology, 38, 305–311.
Boyaval, P., & Goulet, J. (1988). Enzyme and Microbial Technology, 10, 725–728.
Senthuran, A., Senthuran, V., Hatti-Kaul, R., & Mattiasson, B. (1999). Journal of Biotechnology, 73, 61–70.
Lamboley, L., Lacroix, C., Artignan, J. M., Champagne, C. P., & Vuillemard, J. C. (1999). Biotechnology Progress, 15, 646–654.
Lamboley, L., Lacroix, C., Sodini, I., Lemay, M. J., & Champagne, C. P. (2001). Biotechnology Progress, 17, 1071–1078.
Shapiro, L., & Cohen, S. (1997). Biomaterials, 18, 583–590.
Phillips, D. L., Xing, J., Chong, C. K., Liu, H., & Corke, H. (2000). Journal of Agricultural and Food Chemistry, 48, 5105–5108.
Macedo, M. G., Champagne, C. P., Vuillemard, J. C., & Lacroix, C. (1999). International Dairy Journal, 9, 437–445.
Le-Tien, C., Millette, M., Mateescu, M. A., & Lacroix, M. (2004). Biotechnology and Applied Biochemistry, 39, 347–354.
Wurzburg, O. B. (1964). In R. L. Alhistler (Ed.) Methods in carbohydrate chemistry (pp. 286–288). New York: Academic Press.
Bashan, Y. (1986). Applied and Environmental Microbiology, 51, 1089–1098.
John J. Bozzola., & Lonnie D. Russell. (1991) Electron Microscopy: Principles and Techniques for Biologists, Jones & Bartlett Pub ISBN-10: 0867201266.
Lee, K. Y., Bouhadir, K. H., & Mooney, D. J. (2000). Macromolecules, 33, 97–101.
Lee, H. J., Xie, Y., Koo, Y. M., & Wang, N. H. L. (2004). Biotechnology Progress, 20, 179–192.
Hofvendahl, K., & Hahn-Hagerdal, B. (2000). Enzyme and Microbial Technology, 26, 87–107.
Narita, J., Nakahara, S., Fukuda, H., & Kondo, A. (2004). Journal of Biosciences and Bioengineering, 97, 423–425.
Patel, M. A., Ou, M. S., Ingram, L. O., & Shanmugam, K. T. (2005). Biotechnology Progress, 21, 1453–1460.
Acknowledgements
The authors of this investigation acknowledge Dr. Sanjay Nene, Scientist, NCL, Pune for providing L. delbrucekii culture, and Dr. Y Yogeshwara Rao and Dr. Meenakshi Singh of CSIR, New Delhi for financial support under NMITLI programme.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rao, C.S., Prakasham, R.S., Rao, A.B. et al. Functionalized Alginate as Immobilization Matrix in Enantioselective l (+) Lactic Acid Production by Lactobacillus delbrucekii . Appl Biochem Biotechnol 149, 219–228 (2008). https://doi.org/10.1007/s12010-007-8052-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-007-8052-8