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Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS

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Plant Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1072))

Abstract

Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification.

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Acknowledgements

This work was supported in part by Grants-in-Aid for Scientific Research (#22120003 and #24370018) from MEXT, Japan, to Y.K. and M.U.

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Authors and Affiliations

  1. United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate, Japan

    Daisuke Takahashi & Bin Li

  2. Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

    Takato Nakayama, Yukio Kawamura & Matsuo Uemura

Authors
  1. Daisuke Takahashi
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  2. Bin Li
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  3. Takato Nakayama
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  4. Yukio Kawamura
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  5. Matsuo Uemura
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Editor information

Editors and Affiliations

  1. Dept of Biochem & Molecular Bio, University of Cordoba, Cordoba, Spain

    Jesus V. Jorrin-Novo

  2. University of Tsukuba National Institute of Crop Science, Tsukuba, Japan

    Setsuko Komatsu

  3. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Wolfram Weckwerth

  4. Dept. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Stefanie Wienkoop

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Takahashi, D., Li, B., Nakayama, T., Kawamura, Y., Uemura, M. (2014). Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_33

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  • DOI: https://doi.org/10.1007/978-1-62703-631-3_33

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-630-6

  • Online ISBN: 978-1-62703-631-3

  • eBook Packages: Springer Protocols

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