Plant Proteomics

Methods and Protocols

  • Jesus V. Jorrin-Novo
  • Setsuko Komatsu
  • Wolfram Weckwerth
  • Stefanie Wienkoop

Part of the Methods in Molecular Biology book series (MIMB, volume 1072)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Introductory

    1. Front Matter
      Pages 1-1
    2. Jesus V. Jorrin-Novo
      Pages 3-13
    3. Wolfram Weckwerth, Stefanie Wienkoop, Wolfgang Hoehenwarter, Volker Egelhofer, Xiaoliang Sun
      Pages 15-27
    4. Luis Valledor, M. Cristina Romero-Rodríguez, Jesus V. Jorrin-Novo
      Pages 51-60
  3. Experimental

    1. Front Matter
      Pages 83-83
    2. Agnieszka Zienkiewicz, Juan David Rejón, Juan de Dios Alché, María Isabel Rodríguez-García, Antonio Jesús Castro
      Pages 85-91
    3. Raquel González Fernández, Inmaculada Redondo, Jesus V. Jorrin-Novo
      Pages 93-109
    4. Egisto Boschetti, Pier Giorgio Righetti
      Pages 111-129
    5. Sami Irar, Faiçal Brini, Khaled Masmoudi, Montserrat Pagès
      Pages 131-140
    6. Jenny Renaut, Céline Leclercq, Sébastien Planchon
      Pages 141-154
    7. María J. Martínez-Esteso, Juan Casado-Vela, Susana Sellés-Marchart, María A. Pedreño, Roque Bru-Martínez
      Pages 155-169
    8. Martina Vermachova, Zita Purkrtova, Jiri Santrucek, Pascale Jolivet, Thierry Chardot, Milan Kodicek
      Pages 185-198
    9. Manuela Peukert, Michael Becker, Andrea Matros, Hans-Peter Mock
      Pages 223-240

About this book


Plant Proteomics: Methods and Protocols, Second Edition presents recent advances made in the field of proteomics and their application to plant biology and translational research. In recent years, improvements in techniques and protocols for high-throughput proteomics have been made at all workflow stages, from wet (sampling, tissue and cell fractionation, protein extraction, depletion, purification, separation, MS analysis, quantification) to dry lab (experimental design, algorithms for protein identification, bioinformatics tools for data analysis, databases, and repositories). Divided into nine convenient sections, chapters cover topics such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, as well as the study and analysis of PTMs, protein interactions, and specific families of proteins, and finally proteomics in translational research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Plant Proteomics: Methods and Protocols, Second Edition seeks to serve both professionals and novices looking to exploit the full potential of proteomics in plant biology research.


bioinformatics electrophoresis interactomics plant biology proteomics systems biology

Editors and affiliations

  • Jesus V. Jorrin-Novo
    • 1
  • Setsuko Komatsu
    • 2
  • Wolfram Weckwerth
    • 3
  • Stefanie Wienkoop
    • 4
  1. 1.Dept of Biochem & Molecular BioUniversity of CordobaCordobaSpain
  2. 2.University of Tsukuba National Institute of Crop ScienceTsukubaJapan
  3. 3.Molecular Systems BiologyUniversity of ViennaViennaAustria
  4. 4.Dept. Molecular Systems BiologyUniversity of ViennaViennaAustria

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-630-6
  • Online ISBN 978-1-62703-631-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book