Abstract
Mechanical stretch is a major factor for myocardial hypertrophy and heart failure. Stretch activates mechanical sensors from cardiac myocytes, leading to a series of signal transduction cascades, which can result in cell malfunction and remodeling. It is well known that mechanical stretch also induces the release of paracrine factors from cardiac fibroblasts, as well as myocytes. Due to complicated circumstance of heart tissue, it is difficult to fully investigate the characteristics of these factors in situ. Here we describe static stretch and conditioned medium experiments as methods to examine the function of paracrine factors between primary cultured cardiac myocytes and fibroblasts.
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References
Komuro I, Kaida T, Shibazaki Y et al (1990) Stretching cardiac myocytes stimulates protooncogene expression. J Biol Chem 265:3595–3598
Sadoshima J, Jahn L, Takahashi T et al (1992) Molecular characterization of the stretch-induced adaptation of cultured cardiac cells. An in vitro model of load-induced cardiac hypertrophy. J Biol Chem 267:10551–10560
Lal H, Verma SK, Golden HB et al (2008) Stretch-induced regulation of angiotensinogen gene expression in cardiac myocytes and fibroblasts: opposing roles of JNK1/2 and p38alpha MAP kinases. J Mol Cell Cardiol 45:770–778
Camelliti P, Borg TK, Kohl P (2005) Structural and functional characterisation of cardiac fibroblasts. Cardiovasc Res 65:40–51
Golden HB, Gollapudi D, Gerilechaogetu F et al (2012) Isolation of cardiac myocytes and fibroblasts from neonatal rat pups. Methods Mol Biol 843:205–214
Sadoshima J, Izumo S (1993) Mechanical stretch rapidly activates multiple signal transduction pathways in cardiac myocytes: potential involvement of an autocrine/paracrine mechanism. EMBO J 12:1681–1692
Acknowledgments
This manuscript is the result of work supported with resources and the use of facilities at the Central Texas Veterans Health Care System, Temple, Texas. Funding was provided the National Institutes of Health (5R01-HL068838-06), VA Merit Award (IO1 BX000801-01), VA hospital, and Scott and White Hospital.
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© 2013 Springer Science+Business Media, New York
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Feng, H. et al. (2013). Paracrine Communication Between Mechanically Stretched Myocytes and Fibroblasts. In: Baudino, T. (eds) Cell-Cell Interactions. Methods in Molecular Biology, vol 1066. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-604-7_5
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DOI: https://doi.org/10.1007/978-1-62703-604-7_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-603-0
Online ISBN: 978-1-62703-604-7
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