Abstract
Neonatal rat ventricular myocytes (NRVM) and fibroblasts (FBs) serve as in vitro models for studying fundamental mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Both cell types are relatively easy to culture as monolayers and can be manipulated using molecular and pharmacological tools. Because NRVM cease to proliferate after birth, and FBs undergo phenotypic changes and senescence after a few passages in tissue culture, primary cultures of both cell types are required for experiments. Below we describe methods that provide good cell yield and viability of primary cultures of NRVM and FBs from 0 to 3-day-old neonatal rat pups.
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References
Harary, I. and Farley, B. (1963) In vitro studies on single beating rat heart cells. I. Growth and Organization. Experimental Cell Research 29 451–465.
Acknowledgments
This work was supported by the National Institutes of Health (5R01-HL068838-06) and Scott and White Hospital.
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© 2012 Springer Science+Business Media, LLC
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Golden, H.B. et al. (2012). Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_20
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DOI: https://doi.org/10.1007/978-1-61779-523-7_20
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-522-0
Online ISBN: 978-1-61779-523-7
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