Abstract
Stability of gold nanoparticles (AuNPs) is often compromised in physiological conditions. The loss of colloidal stability might lead to undesired biological responses for drug delivery nanosystems. Here a methodology to confer additional stability to the AuNPs by the addition of PEG is presented. Also, protocols to prepare and characterize the composition and conformation of mixed layers with PEG and alkanethiols are described here. Finally, methods to assay the stability of the link between the NP conjugates and a model drug are shown.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Daniel MC, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–346
Rana S, Bajaj A, Mout R, Rotello VM (2012) Monolayer coated gold nanoparticles for delivery applications. Adv Drug Deliv Rev 64:200–216
Ruenraroengsak P, Cook JM, Florence AT (2010) Nanosystem drug targeting: facing up to complex realities. J Control Release 141:265–276
Lim IS, Goroleski F, Mott D, Kariuki N, Ip W et al (2006) Adsorption of cyanine dyes on gold nanoparticles and formation of J-aggregates in the nanoparticle assembly. J Phys Chem B 110:6673–6682
Dobrovolskaia MA, McNeil SE (2007) Immunological properties of engineered nanomaterials. Nat Nanotechnol 2:469–478
Comenge J, Romero FM, Sotelo C, Dominguez F, Puntes V (2010) Exploring the binding of Pt drugs to gold nanoparticles for controlled passive release of cisplatin. J Control Release 148:e31–32
Casals E, Pfaller T, Duschl A, Oostingh GJ, Puntes V (2010) Time evolution of the nanoparticle protein corona. ACS Nano 4:3623–3632
Lipka J, Semmler-Behnke M, Sperling RA, Wenk A, Takenaka S et al (2010) Biodistribution of PEG-modified gold nanoparticles following intratracheal instillation and intravenous injection. Biomaterials 31:6574–6581
Love JC, Estroff LA, Kriebel JK, Nuzzo RG, Whitesides GM (2005) Self-assembled monolayers of thiolates on metals as a form of nanotechnology. Chem Rev 105:1103–1170
Simpson CA, Agrawal AC, Balinski A, Harkness KM, Cliffel DE (2011) Short-chain PEG mixed monolayer protected gold clusters increase clearance and red blood cell counts. ACS Nano 5:3577–3584
Dhar S, Gu FX, Langer R, Farokhzad OC, Lippard SJ (2008) Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt(IV) prodrug-PLGA-PEG nanoparticles. Proc Natl Acad Sci 105:17356–17361
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Comenge, J., Puntes, V.F. (2013). Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_22
Download citation
DOI: https://doi.org/10.1007/978-1-62703-462-3_22
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-461-6
Online ISBN: 978-1-62703-462-3
eBook Packages: Springer Protocols