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Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment

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Nanomaterial Interfaces in Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1025))

Abstract

Nanoparticles (NPs) are being implemented in a wide range of applications, and it is critical to proactively investigate their toxicity. Due to the extensive range of NPs being produced, in vitro studies are a valuable approach for toxicity screening. Key information required to support in vitro toxicity assessments include NP stability in biologically relevant media and fate once exposed to cells. Hyperspectral microscopy is a sensitive, real-time technique that combines the use of microscopy and spectroscopy for the measurement of the reflectance spectrum at individual pixels in a micrograph. This method has been used extensively for molecular imaging with plasmonic NPs as contrast agents (Aaron et al., Opt Express 16:2153−2167, 2008; Kumar et al., Nano Lett 7:1338−1343, 2007; Wax and Sokolov, Laser Photon Rev 3:146−158, 2009; Curry et al., Opt Express 14:6535–6542, 2006; Curry et al., J Biomed Opt 13:014022, 2008; Cognet et al., Proc Natl Acad Sci U S A 100:11350–11355, 2003; Sokolov et al., Cancer Res 63:1999–2004, 2003; Sönnichsen et al., Nat Biotechnol 23:741−745, 2005; Nusz et al., Anal Chem 80:984–989, 2008) and/or sensors (Nusz et al., Anal Chem 80:984–989, 2008; Ungureanu et al., Sens Actuators B 150:529−536, 2010; McFarland and Van Duyne, Nano Lett 3:1057−1062, 2003; Galush et al., Nano Lett 9:2077−2082, 2009; El-Sayed et al., Nano Lett 5:829–834, 2005). Here we describe an approach for using hyperspectral microscopy to characterize the agglomeration and stability of plasmonic NPs in biological media and their interactions with cells.

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Acknowledgments

This work was supported by the Air Force Surgeon General and the Air Force Research Laboratory, Chief Scientist Seedling Program. Christin Grabinski receives a fellowship from the Oak Ridge Institute for Science and Education.

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Authors and Affiliations

  1. Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, USA

    Christin Grabinski, John Schlager & Saber Hussain

Authors
  1. Christin Grabinski
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  2. John Schlager
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  3. Saber Hussain
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Editor information

Editors and Affiliations

  1. Dipto di Ingegneria Meccanica, Università degli Studi di Brescia, Brescia, Italy

    Paolo Bergese

  2. Dept of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    Kimberly Hamad-Schifferli

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Grabinski, C., Schlager, J., Hussain, S. (2013). Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_13

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  • DOI: https://doi.org/10.1007/978-1-62703-462-3_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-461-6

  • Online ISBN: 978-1-62703-462-3

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