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A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis

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Plant Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2061))

Abstract

Plants are powerful model systems to study meiosis. Our knowledge about the cytology of plant meiosis is mainly based on the analysis of fixed material. Although highly informative, this approach is limited in understanding the dynamics of meiosis. Here, we present a step-by-step instruction for a newly developed method to follow meiosis in male meiocytes of Arabidopsis in real time by confocal laser scanning microscopy. We envision that this method can be easily translated to other plant species and especially crops (e.g., Brassica, maize, and potato).

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Acknowledgments

This work was supported by the EU-ITN grant COMREC, the BMBF grant TarBreed, and core funding of the University of Hamburg to A.S.

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Authors and Affiliations

  1. Department of Developmental Biology, University of Hamburg, Hamburg, Germany

    Maria Ada Prusicki, Yuki Hamamura & Arp Schnittger

Authors
  1. Maria Ada Prusicki
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  2. Yuki Hamamura
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  3. Arp Schnittger
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Corresponding author

Correspondence to Arp Schnittger .

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Editors and Affiliations

  1. Departamento de Genética, Fisiología y Microbiología, Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain

    Mónica Pradillo

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Stadt Seeland, Germany

    Stefan Heckmann

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Prusicki, M.A., Hamamura, Y., Schnittger, A. (2020). A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis. In: Pradillo, M., Heckmann, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 2061. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9818-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9818-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9817-3

  • Online ISBN: 978-1-4939-9818-0

  • eBook Packages: Springer Protocols

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