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Cryotherapy: A Novel Method for Virus Eradication in Economically Important Plant Species

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Virus diseases have been a great threat to production of economically important crops. In practice, the use of virus-free planting material is an effective strategy to control viral diseases. Cryotherapy, developed based on cryopreservation, is a novel plant biotechnology tool for virus eradication. Comparing to the traditional meristem culture for virus elimination, cryotherapy resulted in high efficiency of pathogen eradication. In general, cryotherapy includes seven major steps: (1) introduction of infected plant materials into in vitro cultures, (2) shoot tip excision, (3) tolerance induction of explants to dehydration and subsequent freezing in liquid nitrogen (LN), (4) a short-time treatment of explants in LN, (5) warming and post-culture for regeneration, (6) re-establishment of regenerated plants in greenhouse conditions, and (7) virus indexing.

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Acknowledgments

The authors acknowledge financial support from the fund provided by the Department of Science and Technology of Shaanxi Province (2014KTCL02-05).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China

    Min-Rui Wang, Long Chen & Qiao-Chun Wang

  2. Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, Norway

    Min-Rui Wang, Zhibo Zhang & Dag-Ragnar Blystad

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  1. Min-Rui Wang
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  2. Long Chen
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  3. Zhibo Zhang
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  4. Dag-Ragnar Blystad
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  5. Qiao-Chun Wang
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Corresponding author

Correspondence to Qiao-Chun Wang .

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Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Wang, MR., Chen, L., Zhang, Z., Blystad, DR., Wang, QC. (2018). Cryotherapy: A Novel Method for Virus Eradication in Economically Important Plant Species. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_17

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