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Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro

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Glioblastoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1741))

Abstract

To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.

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Acknowledgments

This work was supported by the NIH/NINDS grant R21-NS074055 (D.Z.). We gratefully acknowledge Dr. Mine Esencay for performing the glioma cell immunofluorescence experiment and Scott Kamen for assisting with the manuscript editing and figure preparation.

Author information

Author notes

  1. Jean-Pierre Gagner and Mirna Lechpammer contributed equally to this work.

Authors and Affiliations

  1. Microvascular and Molecular Neuro-Oncology Laboratory, Department of Pathology, NYU Langone Medical Center, New York, NY, USA

    Jean-Pierre Gagner & David Zagzag

  2. Department of Pathology, NYU Langone Medical Center, New York, NY, USA

    Jean-Pierre Gagner & David Zagzag

  3. Department of Pathology and Laboratory Medicine, Division of Neuropathology, Medical Center, University of California, Davis, Sacramento, CA, USA

    Mirna Lechpammer

  4. Division of Neuropathology, Department of Neurosurgery, NYU Langone Medical Center, New York, NY, USA

    David Zagzag

  5. NYU Langone Laura and Isaac Perlmutter Cancer Center, New York, NY, USA

    David Zagzag

Authors
  1. Jean-Pierre Gagner
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  2. Mirna Lechpammer
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  3. David Zagzag
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Corresponding author

Correspondence to David Zagzag .

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, NYU School of Medicine, New York, New York, USA

    Dimitris G. Placantonakis

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Gagner, JP., Lechpammer, M., Zagzag, D. (2018). Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro. In: Placantonakis, D. (eds) Glioblastoma. Methods in Molecular Biology, vol 1741. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7659-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7659-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7658-4

  • Online ISBN: 978-1-4939-7659-1

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