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Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways

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Transcription Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 647))

Abstract

Hypoxia-inducible factor-1 (HIF-1) has a central role in the mammalian program by which cells respond to hypoxia in both physiological and pathological situations. HIF-1 transcriptional activity, protein stabilization, protein–protein interaction, and cellular localization are mainly modulated by Post-translational modifications such as hydroxylation, acetylation, phosphorylation, S-nitrosylation, and SUMOylation. Here, we summarize current knowledge about Post-translational HIF-1 regulation and give additional information about useful methods to determine some of these various modifications.

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Acknowledgments

Our studies were supported by grants from Fonds der Chemischen Industrie and Deutsche Krebshilfe 106929.

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Authors and Affiliations

  1. Department of Chemistry/Biochemistry, University of Kaiserslautern, Kaiserslautern, Germany

    Elitsa Y. Dimova & Thomas Kietzmann

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  1. Elitsa Y. Dimova
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  2. Thomas Kietzmann
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Correspondence to Thomas Kietzmann .

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Editors and Affiliations

  1. Ctr. Cell Biology & Cancer Research, Albany Medical College, New Scotland Ave. 47, Albany, 12208, New York, USA

    Paul J. Higgins

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Dimova, E.Y., Kietzmann, T. (2010). Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways. In: Higgins, P. (eds) Transcription Factors. Methods in Molecular Biology, vol 647. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-738-9_13

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  • DOI: https://doi.org/10.1007/978-1-60761-738-9_13

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  • Publisher Name: Humana Press, Totowa, NJ

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  • Online ISBN: 978-1-60761-738-9

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