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Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast

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Schizosaccharomyces pombe

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1721))

Abstract

Fission yeast is a single-cell eukaryote that has been used extensively as a model organism to study cell biology and virology of higher eukaryotes including plants and humans. In particular, it is a very well-tested model to study evolutionary highly conserved cellular activities such as cell proliferation, cell cycle regulation, and cell death. Some of the advantages of using fission yeast as a surrogate system: easy to carry out functional and genome-wide analysis of small viral genome, easy to maintain in the laboratory with a relatively short doubling time. It is genetically amendable and can be used to test the effect of gain-of-function or loss-of-function of a gene product. Here, we describe a streamlined and large-scale molecular cloning strategy for genome-wide characterization of small viruses in fission yeast.

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Acknowledgments

This work is supported in part by an intramural fund from the University of Maryland Medical Center (R.Y.Z.).

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Authors and Affiliations

  1. University of Maryland School of Medicine, Baltimore, MD, USA

    Ge Li & Richard Y. Zhao

Authors
  1. Ge Li
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  2. Richard Y. Zhao
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Corresponding author

Correspondence to Richard Y. Zhao .

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Editors and Affiliations

  1. Department of Biological Sciences, Winston-Salem State University, Winston-Salem, North Carolina, USA

    Teresa L. Singleton

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Li, G., Zhao, R.Y. (2018). Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast. In: Singleton, T. (eds) Schizosaccharomyces pombe. Methods in Molecular Biology, vol 1721. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7546-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7546-4_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7545-7

  • Online ISBN: 978-1-4939-7546-4

  • eBook Packages: Springer Protocols

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