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Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal Cord

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Biochemical Approaches for Glutamatergic Neurotransmission

Part of the book series: Neuromethods ((NM,volume 130))

Abstract

Glutamate has a paramount role in central nervous system since it is the main excitatory neurotransmitter in both the brain and the spinal cord. In clinical studies, the monitoring of glutamate has revealed tight links between the variations of its interstitial concentration and those of other metabolic biomarkers and partial pressure in oxygen within tissue. The use of microdialysis has allowed to monitor glutamate over several days in patients with brain injury, epilepsy, or Parkinson’s disease. We describe here a neurochemical method carried out in the spinal cord of patients suffering from chronic pain compared to patients suffering from spasticity, with a stress on peculiarities on such a monitoring. We provide also a detailed discussion on the significant usefulness of microdialysis and its technical and methodological limits in the specific field of clinical research.

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Authors and Affiliations

  1. INSERM U1028, CNRS UMR5292, Université Lyon 1, Lyon Neuroscience Research Center, Lyon, France

    Bernard Renaud

  2. Laboratoire de Biochimie et Biologie Moléculaire, Groupement Hospitalier Sud, Centre de Biologie et de Pathologie Sud, Hospices Civils de Lyon, Pierre-Bénite, France

    Delphine Collin-Chavagnac

  3. Department of Neurosurgery, Department of Anatomy - INSERM 1028, Hospices Civils de Lyon, Lyon 1 University, Lyon, France

    Patrick Mertens

  4. CNRS UMR5292, INSERM U1028, University Lyon 1, Lyon Neuroscience Research Center, NeuroDialyTics Unit, 7 rue Guillaume Paradin, Lyon, 69372, France

    Luc Denoroy & Sandrine Parrot

Authors
  1. Bernard Renaud
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  2. Luc Denoroy
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  3. Delphine Collin-Chavagnac
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  4. Patrick Mertens
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  5. Sandrine Parrot
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Corresponding author

Correspondence to Sandrine Parrot .

Editor information

Editors and Affiliations

  1. Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Sandrine Parrot

  2. Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Luc Denoroy

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Renaud, B., Denoroy, L., Collin-Chavagnac, D., Mertens, P., Parrot, S. (2018). Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal Cord. In: Parrot, S., Denoroy, L. (eds) Biochemical Approaches for Glutamatergic Neurotransmission. Neuromethods, vol 130. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7228-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7228-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7227-2

  • Online ISBN: 978-1-4939-7228-9

  • eBook Packages: Springer Protocols

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