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Visualizing In Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy

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Fibrosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1627))

Abstract

Techniques and protocols for the in vitro formation of collagen type I fibrils and the extensive biochemical variation of the fibrillogenesis conditions are presented. In all cases, the incubation and fibrillogenesis product can be readily monitored by transmission electron microscopic study of negatively stained specimens. Representative TEM data is presented and discussed within the context of the products of the fibrillogenesis protocols, from which the extensive biochemical and structural possibilities of this integrated approach can be appreciated.

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References

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Acknowledgments

This and related work was performed by the author as a scientific staff member in the laboratories of the Institute of Zoology, University of Mainz, Germany, with the support of Professor Juergen Markl, and also in retirement using equipment in EM Research Services at Newcastle University, UK, with the support of Professor Richard J. Lewis, Institute of Cellular and Molecular Biosciences.

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Authors and Affiliations

  1. Institute of Zoology, University of Mainz, Mainz, Germany

    J. Robin Harris

  2. 11 Hackwood Park, Hexham, UK

    J. Robin Harris

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  1. J. Robin Harris
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Corresponding author

Correspondence to J. Robin Harris .

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Editors and Affiliations

  1. Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, USA

    Laure Rittié

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Harris, J.R. (2017). Visualizing In Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy. In: Rittié, L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7113-8_24

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  • DOI: https://doi.org/10.1007/978-1-4939-7113-8_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7112-1

  • Online ISBN: 978-1-4939-7113-8

  • eBook Packages: Springer Protocols

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