Methods and Protocols

  • Laure Rittié

Part of the Methods in Molecular Biology book series (MIMB, volume 1627)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Joel Rosenbloom, Edward Macarak, Sonsoles Piera-Velazquez, Sergio A. Jimenez
    Pages 1-23
  3. Animal Models of Fibrosis

    1. Front Matter
      Pages 25-25
    2. Tianju Liu, Francina Gonzalez De Los Santos, Sem H. Phan
      Pages 27-42
    3. Sabine Klein, Robert Schierwagen, Frank Erhard Uschner, Jonel Trebicka
      Pages 91-116
    4. Laure Rittié, Audrey E. K. Hutcheon, James D. Zieske
      Pages 117-122
    5. Daniela Fraccarollo, Paolo Galuppo, Johann Bauersachs
      Pages 123-137
    6. Ian M. H. Li, Amy L. Horwell, Grace Chu, Benoit de Crombrugghe, George Bou-Gharios
      Pages 139-161
  4. Cell Culture Models

    1. Front Matter
      Pages 163-163
    2. Sabine Weiskirchen, Carmen G. Tag, Sibille Sauer-Lehnen, Frank Tacke, Ralf Weiskirchen
      Pages 165-191
    3. Lawrence J. Liew, Huan Ting Ong, Rodney J. Dilley
      Pages 193-203
    4. Tripp Leavitt, Michael S. Hu, Michael T. Longaker
      Pages 205-212
    5. Nahum Puebla-Osorio, Seri N. E. Sarchio, Stephen E. Ullrich, Scott N. Byrne
      Pages 213-222
    6. Beate Eckes, Fang Wang, Laure Rittié, Gabriele Scherr, Paola Zigrino
      Pages 223-233
    7. Tianfa Xie, Jamar Hawkins, Yubing Sun
      Pages 235-244
    8. Christal A. Worthen, Laure Rittié, Gary J. Fisher
      Pages 245-251

About this book


This volume describes state-of-the-art protocols that serve as “recipes” for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.


bleomycin Myocardial infarction fibroblasts mesenchymal stem cells adipose cell culture systems ECM proteins

Editors and affiliations

  • Laure Rittié
    • 1
  1. 1.Department of DermatologyUniversity of Michigan Medical SchoolAnn ArborUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7112-1
  • Online ISBN 978-1-4939-7113-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book