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CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry

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Protein-Carbohydrate Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1588))

Abstract

Immunocytochemistry is a widely used technique to localize antigen within intact tissues. Plant cell walls are complex matrixes of highly decorated polysaccharides and the large number of CBM families displaying specific substrate recognition reflects this complexity. The accessibility of large proteins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry in muro and to quantify enzymatic deconstruction.

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Acknowledgments

We acknowledge Alain Jauneau and Aurelie Le Ru for technical assistance on Nanozoomer RS provided by the TRI-genotoul facility (http://trigenotoul.com/).

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Authors and Affiliations

  1. LISBP, Université de Toulouse, CNRS, INRA, INSA, 135 Avenue de Rangueil, 31077, Toulouse, France

    Louise Badruna & Cédric Y. Montanier

  2. Laboratoire de Recherche en Sciences Végétales, UMR 5546 UPS/CNRS, 31326, Castanet-Tolosan, France

    Vincent Burlat

Authors
  1. Louise Badruna
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  2. Vincent Burlat
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  3. Cédric Y. Montanier
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Correspondence to Cédric Y. Montanier .

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Editors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada

    D. Wade Abbott

  2. Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    Alicia Lammerts van Bueren

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Badruna, L., Burlat, V., Montanier, C.Y. (2017). CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry. In: Abbott, D., Lammerts van Bueren, A. (eds) Protein-Carbohydrate Interactions. Methods in Molecular Biology, vol 1588. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6899-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6899-2_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6898-5

  • Online ISBN: 978-1-4939-6899-2

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