Summary
Enzyme-gold complexes have been prepared with an endo β 1→4 xylanase (EC 3.2.1.8) and a β 1→4 mannanase (EC 3.2.1.78). The complexes were applied to ultrathin sections of plant cell walls for the ultrastructural localization of xylans in different tissues of a graminea and for the localization of glucomannans in the tracheids of spruce wood. The method proved to be highly specific and gave a very good contrast of the substrate polysaccharides. Used in conjunction with other cytochemical staining the enzyme-gold labelling provided information about the relative distribution of pectic polymers and xylans in primary walls.
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Ruel, K., Joseleau, J.P. Use of enzyme-gold complexes for the ultrastructural localization of hemicelluloses in the plant cell wall. Histochemistry 81, 573–580 (1984). https://doi.org/10.1007/BF00489537
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DOI: https://doi.org/10.1007/BF00489537