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Chapter 3 Current Challenges in Immature Oocyte Cryopreservation

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Cryopreservation of Mammalian Gametes and Embryos

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

Abstract

Current freezing technology, especially the vitrification method, has markedly improved oocyte survival rate after warming, and the pregnancy rate is comparable to that achieved with fresh oocytes. However, most groups report using oocytes matured in vivo for vitrification. Although immature oocytes can be vitrified successfully, clinical outcomes do not reach that of vitrification of matured oocytes. The current literature suggests that oocytes should be vitrified at mature metaphase II (M-II) stage following IVM rather than at the immature germinal vesicle (GV) stage, because the potential for oocyte maturation is reduced when vitrification is performed on immature oocytes at the GV stage.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, McGill University, 687 Pine Avenue West, H3A 1A1, Montreal, Canada

    Ri-Cheng Chian, Yixin Xu & Dana Keilty

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  1. Ri-Cheng Chian
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  2. Yixin Xu
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  3. Dana Keilty
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Correspondence to Ri-Cheng Chian .

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Editors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia, USA

    Zsolt Peter Nagy

  2. ASTRA Fertility Group, Mississauga, Ontario, Canada

    Alex C. Varghese

  3. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Ashok Agarwal

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Chian, RC., Xu, Y., Keilty, D. (2017). Chapter 3 Current Challenges in Immature Oocyte Cryopreservation. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_3

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