Cryopreservation of Mammalian Gametes and Embryos

Methods and Protocols

  • Zsolt Peter Nagy
  • Alex C. Varghese
  • Ashok Agarwal

Part of the Methods in Molecular Biology book series (MIMB, volume 1568)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Jaffar Ali, Naif H. AlHarbi, Nafisa Ali
      Pages 3-20
  3. Basics and Advanced Biology

    1. Front Matter
      Pages 21-21
    2. Seul Ki Kim, Jung Ryeol Lee, S. Samuel Kim
      Pages 23-31
    3. Ri-Cheng Chian, Yixin Xu, Dana Keilty
      Pages 33-44
    4. Seul Ki Kim, Hye Won Youm, Jung Ryeol Lee, Chang Suk Suh
      Pages 45-63
  4. Methodology

    1. Front Matter
      Pages 65-65
    2. Ashok Agarwal, Eva Tvrda
      Pages 67-78
    3. Vladimir Isachenko, Raul Sanchez, Peter Mallmann, Gohar Rahimi, Evgenia Isachenko
      Pages 79-84
    4. Pankaj Talwar, Sarabpreet Singh
      Pages 85-104
    5. David H. Edgar, Janell Archer, Debra A. Gook
      Pages 119-129
    6. Laura Rienzi, Ana Cobo, Filippo Maria Ubaldi
      Pages 131-139
    7. Juergen Liebermann
      Pages 141-159
    8. Debra A. Gook
      Pages 161-176
    9. Sherman Silber
      Pages 177-194
    10. James Graham, Josh Lim, Michael Tucker
      Pages 195-203
  5. Advances in Cryotechnology, Research, and Animal Models

    1. Front Matter
      Pages 221-221

About this book


This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation. 


Embryo cryopreservation Embryo vitrification Cryobiology Human oocytes ovarian and testicular tissues fertility reproductive cells

Editors and affiliations

  • Zsolt Peter Nagy
    • 1
  • Alex C. Varghese
    • 2
  • Ashok Agarwal
    • 3
  1. 1.Reproductive Biology AssociatesAtlantaUSA
  2. 2.ASTRA Fertility GroupMississaugaCanada
  3. 3.Cleveland Clinic FoundationClevelandUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6826-8
  • Online ISBN 978-1-4939-6828-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book