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Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii

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Recombinant Proteins from Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1385))

Abstract

Chloroplast transformation in the green algae Chlamydomonas reinhardtii can be used for the production of valuable recombinant proteins. Here, we describe chloroplast transformation of C. reinhardtii followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant proteins can be detected by conventional techniques like immunoblotting and purified from liquid cultures.

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Acknowledgements

SIP-IPN 20144606 and 20144617 support research in the laboratory of J.A.B.C. and N.V.D.F., respectively. ICYT-DF PIUTE-10-74 and PICSA-10-1974 support research in the laboratory of J.A.B.C. J.A.B.C. and N.V.D.F. are EDI and COFAA fellows. K.S.M.O., D.G.Z., and A.L.A.D. are recipients of a PhD scholarship from CONACYT-México.

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Authors and Affiliations

  1. Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto SN, Col. Barrio La Laguna Ticomán, CP. 07340, Mexico City, Mexico

    Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena Almaraz-Delgado, Noé Durán-Figueroa & Jesus Agustín Badillo-Corona

Authors
  1. Daniel Guzmán-Zapata
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  2. Karla Soledad Macedo-Osorio
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  3. Alma Lorena Almaraz-Delgado
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  4. Noé Durán-Figueroa
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  5. Jesus Agustín Badillo-Corona
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Corresponding author

Correspondence to Jesus Agustín Badillo-Corona .

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Editors and Affiliations

  1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada

    Jacqueline MacDonald

  2. Department of Biology, Western University, London, Ontario, Canada

    Igor Kolotilin

  3. London, Ontario, Canada

    Rima Menassa

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Guzmán-Zapata, D., Macedo-Osorio, K.S., Almaraz-Delgado, A.L., Durán-Figueroa, N., Badillo-Corona, J.A. (2016). Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii . In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3289-4_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3288-7

  • Online ISBN: 978-1-4939-3289-4

  • eBook Packages: Springer Protocols

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