Recombinant Proteins from Plants

Methods and Protocols

  • Jacqueline MacDonald
  • Igor Kolotilin
  • Rima Menassa

Part of the Methods in Molecular Biology book series (MIMB, volume 1385)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Vectors and Transformation

    1. Front Matter
      Pages 1-1
    2. Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, George P. Lomonossoff
      Pages 39-54
    3. Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa McNulty, Kahlin Leuzinger et al.
      Pages 55-67
    4. Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena Almaraz-Delgado, Noé Durán-Figueroa, Jesus Agustín Badillo-Corona
      Pages 69-85
  3. Product Improvements

    1. Front Matter
      Pages 87-87
    2. Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini
      Pages 89-98
    3. Stéphanie Robert, Philippe V. Jutras, Moustafa Khalf, Marc-André D’Aoust, Marie-Claire Goulet, Frank Sainsbury et al.
      Pages 115-126
  4. Extraction and Scale-Up

  5. Analysis

    1. Front Matter
      Pages 199-199
    2. Kurniawan Rudi Trijatmiko, Felichi Mae Arines, Norman Oliva, Inez Hortense Slamet-Loedin, Ajay Kohli
      Pages 201-222

About this book


This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.


cloning molecular farming plant expression systems plants recombinant proteins transgenic plants

Editors and affiliations

  • Jacqueline MacDonald
    • 1
  • Igor Kolotilin
    • 2
  • Rima Menassa
    • 3
  1. 1.Southern Crop Protection and Food Research CentreAgriculture and Agri-Food CanadaLondonCanada
  2. 2.Department of BiologyWestern UniversityLondonCanada
  3. 3.LondonCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3288-7
  • Online ISBN 978-1-4939-3289-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book