Book Volume 1385 2016

Recombinant Proteins from Plants

Methods and Protocols

Editors:

ISBN: 978-1-4939-3288-7 (Print) 978-1-4939-3289-4 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. Vectors and Transformation

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-27

      Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology

    3. No Access

      Protocol

      Pages 29-37

      Multigene Engineering in Rice Using High-Capacity Agrobacterium tumefaciens BIBAC Vectors

    4. No Access

      Protocol

      Pages 39-54

      Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

    5. No Access

      Protocol

      Pages 55-67

      Transient Protein Expression by Agroinfiltration in Lettuce

    6. No Access

      Protocol

      Pages 69-85

      Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii

  3. Product Improvements

    1. Front Matter

      Pages 87-87

    2. No Access

      Protocol

      Pages 89-98

      Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes

    3. No Access

      Protocol

      Pages 99-113

      Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation

    4. No Access

      Protocol

      Pages 115-126

      Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

  4. Extraction and Scale-Up

    1. Front Matter

      Pages 127-127

    2. No Access

      Protocol

      Pages 129-137

      Production of Recombinant Cholera Toxin B Subunit in Nicotiana benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector

    3. No Access

      Protocol

      Pages 139-147

      Total Soluble Protein Extraction for Improved Proteomic Analysis of Transgenic Rice Plant Roots

    4. No Access

      Protocol

      Pages 149-160

      Temporary Immersion Bioreactors for the Contained Production of Recombinant Proteins in Transplastomic Plants

    5. No Access

      Protocol

      Pages 161-172

      Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor

    6. No Access

      Protocol

      Pages 173-188

      Liquid-Liquid Phase Separation of Oil Bodies from Seeds

    7. No Access

      Protocol

      Pages 189-197

      Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract

  5. Analysis

    1. Front Matter

      Pages 199-199

    2. No Access

      Protocol

      Pages 201-222

      Molecular Analyses of Transgenic Plants

    3. No Access

      Protocol

      Pages 223-247

      Analysis of Recombinant Proteins in Transgenic Rice Seeds: Identity, Localization, Tolerance to Digestion, and Plant Stress Response

    4. No Access

      Protocol

      Pages 249-257

      Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2

    5. No Access

      Protocol

      Pages 259-270

      A Standardized Lepidopteran Bioassay to Investigate the Bioactivity of Insecticidal Proteins Produced in Transgenic Crops

  6. Antibodies for Pathogen Resistance

    1. Front Matter

      Pages 271-271

    2. No Access

      Protocol

      Pages 273-291

      Antibody-Mediated Pathogen Resistance in Plants

  7. No Access

    Protocol

    Pages E1-E1

    Erratum to: Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology

  8. Back Matter

    Pages 293-296