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Fluorescence Staining of Mitochondria for Morphology Analysis in Saccharomyces cerevisiae

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Yeast Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1163))

Abstract

Mitochondria are highly dynamic organelles in all eukaryotic cells. Most of our insights regarding the mechanisms that determine the morphogenesis and motility of mitochondria have been identified and analyzed first in the model organism Saccharomyces cerevisiae. To this end high-resolution microscopic methods were applied that rely on fluorescence labeling of the organelle. A comprehensive overview of fluorescence staining approaches that were successfully applied to study the behavior of mitochondria in vivo but also in fixed cells is provided. Slightly modified versions of the methods described here can also be used to analyze other compartments of the yeast cell. Microscopic setups and imaging methods will only be shortly discussed since these are highly dependent on each laboratory’s basic infrastructure.

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Acknowledgements

I am grateful to Doron Rapaport for his continuous support and for valuable suggestions to the manuscript. Furthermore I thank Tao Tan for his contributions to the fluorescence microscopy images. Work in the laboratory was supported by the fortüne program of the medical faculty of the University of Tübingen.

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Authors and Affiliations

  1. Interfaculty Institute of Biochemistry, University of Tübingen, Hoppe-Seyler-Str. 4, 72076, Tübingen, Germany

    Kai Stefan Dimmer

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  1. Kai Stefan Dimmer
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Correspondence to Kai Stefan Dimmer .

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Editors and Affiliations

  1. Capital Normal University College of Life Sciences, Beijing, China

    Wei Xiao

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Dimmer, K.S. (2014). Fluorescence Staining of Mitochondria for Morphology Analysis in Saccharomyces cerevisiae . In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 1163. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0799-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-0799-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0798-4

  • Online ISBN: 978-1-4939-0799-1

  • eBook Packages: Springer Protocols

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